Minor activities and transition state properties of the human steroid hydroxylases cytochromes P450c17 and P450c21, from reactions observed with deuterium-labeled substrates

Biochemistry

Volumn 51, Issue 36, 2012, Pages 7064-7077

  Yoshimoto, Francis K ^a,b,c   Zhou, Yishan ^a   Peng, Hwei Ming ^c   Stidd, David ^a   Yoshimoto, Jennifer A ^c   Sharma, Kamalesh K ^a   Matthew, Susan ^c   Auchus, Richard J ^a,b,c  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b UNIVERSITY OF TEXAS AT DALLAS   (United States)

^c UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

C-H BOND; C-H BOND CLEAVAGE; DEUTERIUM RETENTION; FORMATION RATES; HYDROGEN ATOM ABSTRACTION; HYDROXYLASE ACTIVITY; HYDROXYLASES; INTERMOLECULAR KINETICS; MINOR ACTIVITY; OXIDOREDUCTASES; PREGNENOLONE; PRODUCT DISTRIBUTIONS; RATE LIMITING; REGIOCHEMICAL; STEREOCHEMICAL INVERSION; TRANSITION STATE; WILD TYPES;

DEUTERIUM; HYDROGEN; HYDROXYLATION;

SUBSTRATES;

CHOLESTEROL 7ALPHA MONOOXYGENASE; CYTOCHROME P450 REDUCTASE; CYTOCHROME P450C17; DEUTERIUM; HYDROGEN; HYDROXYPROGESTERONE; PREGNENOLONE; PROGESTERONE; STEROID 16ALPHA MONOOXYGENASE; STEROID 21 MONOOXYGENASE; VALINE;

ARTICLE; CHEMICAL STRUCTURE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME BINDING; ENZYME INHIBITION; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SYNTHESIS; HYDROGEN BOND; HYDROXYLATION; MUTATION; NONHUMAN; OXYGENATION; PRIORITY JOURNAL; STEREOCHEMISTRY; WILD TYPE;

DEUTERIUM; HUMANS; KINETICS; PROTEIN BINDING; STEROID 17-ALPHA-HYDROXYLASE; STEROID 21-HYDROXYLASE; STEROIDS;

EID: 84866133140     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi300895w     Document Type: Article

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