Cytochrome P450 7A1 cholesterol 7α-hydroxylation: Individual reaction steps in the catalytic cycle and rate-limiting ferric iron reduction

Journal of Biological Chemistry

Volumn 286, Issue 6, 2011, Pages 4632-4643

  Shinkyo, Raku ^a   Peter Guengerich, F ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

APPARENT K; BILE ACID; CATALYTIC ACTIVITY; CATALYTIC CYCLES; COMPETITIVE KINETICS; CYTOCHROME P450; DEUTERIUM ISOTOPE EFFECT; ELECTRON-TRANSFER STEP; FERRIC IRON; HUMAN ENZYMES; HYDROXYLASES; KINETIC MODELS; RAPID REDUCTION; RATE LIMITING; REACTION STEPS; SUBSTRATE OXIDATION;

CARRIER MOBILITY; CATALYST ACTIVITY; DEUTERIUM; ENZYMES; HYDROXYLATION; IRON; MAMMALS; REACTION RATES; SYNTHESIS (CHEMICAL);

CHOLESTEROL;

CHOLESTEROL; CHOLESTEROL 7ALPHA MONOOXYGENASE; DEUTERIUM; FERRIC ION; HYDROGEN PEROXIDE; CYP7A1 PROTEIN, HUMAN; CYP7A1 PROTEIN, RAT; IRON; OXYGEN;

ARTICLE; BINDING COMPETITION; CATALYSIS; CONTROLLED STUDY; ENZYME BINDING; ENZYME MECHANISM; ENZYME SUBSTRATE COMPLEX; HUMAN; HUMAN CELL; HYDROGEN BOND; HYDROXYLATION; ISOTOPE ANALYSIS; LIPID ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN LIPID INTERACTION; REDUCTION KINETICS; ANIMAL; CHEMICAL MODEL; CHEMISTRY; DEUTERIUM HYDROGEN EXCHANGE; GENETICS; KINETICS; METABOLISM; OXIDATION REDUCTION REACTION; PHYSIOLOGY; RAT;

MAMMALIA;

ANIMALS; CATALYSIS; CHOLESTEROL; CHOLESTEROL 7-ALPHA-HYDROXYLASE; DEUTERIUM; DEUTERIUM EXCHANGE MEASUREMENT; HUMANS; HYDROGEN PEROXIDE; HYDROXYLATION; IRON; KINETICS; MODELS, CHEMICAL; OXIDATION-REDUCTION; OXYGEN; RATS;

EID: 79953003310     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M110.193409     Document Type: Article

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