Enzymes for the homeland defense: Optimizing phosphotriesterase for the hydrolysis of organophosphate nerve agents

Biochemistry

Volumn 51, Issue 32, 2012, Pages 6463-6475

  Tsai, Ping Chuan ^a   Fox, Nicholas ^a   Bigley, Andrew N ^a   Harvey, Steven P ^b   Barondeau, David P ^a   Raushel, Frank M ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

^b EDGEWOOD CHEMICAL BIOLOGICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICAL WARFARE AGENTS; DIRECTED EVOLUTION; EXPECTED IMPROVEMENTS; HOMELAND DEFENSE; NERVE AGENTS; ORGANOPHOSPHATE PESTICIDES; PHOSPHOTRIESTERASES; PROFICIENT ENZYMES; RATIONAL DESIGN; SELECTION TECHNIQUES; SITE-SPECIFIC MODIFICATIONS; SOIL BACTERIUM; SUBSTRATE BINDING; WILD-TYPE ENZYMES; X RAY CRYSTAL STRUCTURES;

CHEMICAL WARFARE; CHIRALITY; DETOXIFICATION; ENANTIOMERS; ENZYMES; HYDROLYSIS; PHOSPHORUS; SITE SELECTION; STEREOCHEMISTRY;

CATALYST ACTIVITY;

CHEMICAL WARFARE AGENT; CYCLOSARIN; ENZYME VARIANT; METHYLPHOSPHONOTHIOIC ACID S (2 DIISOPROPYLAMINOETHYL) O ETHYL ESTER; NERVE GAS; ORGANOPHOSPHATE; ORGANOPHOSPHATE PESTICIDE; PHOSPHORUS; PHOSPHOTRIESTERASE; SARIN; SOMAN; TABUN;

ARTICLE; CRYSTAL STRUCTURE; DETOXIFICATION; ENANTIOMER; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SUBSTRATE COMPLEX; HYDROLYSIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; SCREENING; SOIL MICROFLORA; STEREOISOMERISM; STEREOSPECIFICITY; WILD TYPE;

BACTERIAL PROTEINS; CATALYSIS; CATALYTIC DOMAIN; CHEMICAL WARFARE AGENTS; CRYSTALLOGRAPHY, X-RAY; ENTEROBACTER AEROGENES; GENE LIBRARY; HIGH-THROUGHPUT SCREENING ASSAYS; HYDROLYSIS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; ORGANOPHOSPHORUS COMPOUNDS; PHOSPHORIC DIESTER HYDROLASES; PHOSPHORIC TRIESTER HYDROLASES; RECOMBINANT PROTEINS; SARIN; SOMAN; STEREOISOMERISM;

EID: 84865146694     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi300811t     Document Type: Article

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