Functional analysis of organophosphorus hydrolase variants with high degradation activity towards organophosphate pesticides

Protein Engineering, Design and Selection

Volumn 19, Issue 3, 2006, Pages 99-105

  Cho, Catherine Mee Hie ^a,b   Mulchandani, Ashok ^a   Chen, Wilfred ^a  

^a University of California Riverside   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Degradation; Directed evolution; Methyl parathion; Organophosphate pesticides; Organophosphorus hydrolase; Site directed mutagenesis

Indexed keywords

HYDROGEN BONDS; HYDROLYSIS; MUTAGENESIS; PHOSPHATES;

DIRECTED EVOLUTION; METHYL PARATHION; ORGANOPHOSPHATE PESTICIDES; ORGANOPHOSPHORUS HYDROLASE; SITE-DIRECTED MUTAGENESIS;

PESTICIDES;

ENZYME VARIANT; ORGANOPHOSPHATE PESTICIDE; ORGANOPHOSPHORUS COMPOUND; ORGANOPHOSPHORUS HYDROLASE; PARATHION METHYL; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DEGRADATION; ENZYME ACTIVITY; ENZYME SPECIFICITY; ESCHERICHIA COLI; HYDROGEN BOND; HYDROLYSIS; MUTATION; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; SUBSTITUTION REACTION;

AMINO ACID SUBSTITUTION; ARYLDIALKYLPHOSPHATASE; BINDING SITES; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; HYDROGEN BONDING; HYDROLYSIS; KINETICS; MODELS, MOLECULAR; MOLECULAR STRUCTURE; ORGANOPHOSPHORUS COMPOUNDS; PESTICIDES; PLASMIDS; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PROTEIN STRUCTURE, SECONDARY; SUBSTRATE SPECIFICITY; VARIATION (GENETICS);

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 33644984271     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzj007     Document Type: Article

References (22)

1. Benning,M.M., Kuo,J.M., Raushel,F.M. and Holden,H.M. (1995) Biochemistry, 34, 7973-7978.
2. Benning,M.M., Hong,S.-B., Raushel,F.M. and Holden,H.M. (2000) J. Biol. Chem., 275, 30556-30560.
3. Cho,C.M.-H, Mulchandani,A. and Chen,W. (2002) Appl. Environ. Microbiol., 68, 2026-2030.
4. Cho,C.M-H., Mulchandani,A. and Chen,W. (2004) Appl. Environ. Microbiol., 70, 4681-4685.
5. Di Sioudi,B.D., Miller,C.E., Lai,K. Grimsley,J.K. and Wild,J.R. (1999) Chem. Biol. Interact., 119-120, 211-223.
6. Dumas,D.P., Caldwell,S.R., Wild,J.R. and Raushel,F.M. (1989) J. Biol. Chem., 261, 19659-19665.
7. Dumas,D.P., Durst,H.D., Landis,W.G., Raushel,F.M. and Wild,J.R. (1990) Arch. Biochem. Biophys., 227, 155-159.
8. Griffiths,A.D. and Tawfik,D.S. (2003) EMBO J., 22, 24-35.
9. Guex,N. and Peitsch,M.C. (1997) Electrophoresis, 18, 2714-2723.
10. Horne,I., Sutherland,T.D., Harcourt,R.L., Russell,R.J. and Oakeshott,J.G. (2002) Appl. Environ. Microbiol., 68, 3371-3376.
11. Jackson,C.J., Liu,J.W., Coote,M.L. and Ollis,D.L. (2005) Organic and Biomol. Chem., 3, 4343-4350.
12. Koca,J., Zhan, C.-G, Rittenhouse,R.C. and Ornstein,R.L. (2001) J. Am. Chem. Soc., 123, 817-826.
13. Kolakowski,J.E., DeFrank,J.J., Harvey,S.P., Szafraniec,L.L., Beaudry,W.T., Lai,K.H. and Wild,J.R. (1997) Biocatalysis and Biotransformation, 15, 297-312.
14. Lai,K., Dave,K.I. and Wild,J.R. (1994) J. Biol. Chem., 269, 16579-16584.
15. McLoughlin,S.Y., Jackson,C., Liu,J.-W. and Ollis,D. (2005) Protein Expr. Purif., 41, 433-440.
16. Roodveldt,C. and Tawfik,D.S. (2005) Protein Eng. Des. Sel., 18, 51-58.
17. Schwede,T., Kopp,J., Guex,N. and Peitsch,M.C. (2003) Nucleic Acids Res., 31, 3381-3385.
18. Shimazu,M., Mulchandani,A. and Chen,W. (2001) Biotechnol. Prog., 17, 76-80.
19. Vanhooke,J.L., Benning,M.N., Raushel,F.M.and Golden,H.M. (1996) Biochemistry, 35, 6020-6025.
20. Watkins,L.M., Mahoney,J.J., McCulloch,J.K. and Raushel,F.M. (1997) J. Biol. Chem., 272, 25596-25601.
21. Wu,F., Li,W-S., Chen-Goodspeed,M., Sogorb,M.A., Raushel,F.M. (2000) J. Am. Chem. Soc., 122, 10206-10207.
22. Yang,H., Carr,P.D., McLoughlin,S.Y., Liu,J.W., Horne,I., Qiu,X., Jeffries,C.M.J., Russell,R.J., Oakeshott,J.G. and Ollis,D.L. (2003) Protein Eng., 6, 135-145.