Enhanced thermostability of a Rhizopus chinensis lipase by in vivo recombination in Pichia pastoris

Microbial Cell Factories

Volumn 11, Issue , 2012, Pages

  Yu, Xiao Wei ^a   Wang, Rui ^a   Zhang, Meng ^a   Xu, Yan ^a   Xiao, Rong ^a,b  

^a JIANGNAN UNIVERSITY   (China)

^b RUTGERS UNIVERSITY   (United States)

Author keywords

Directed evolution; Lipase; Mutant library construction; Pichia pastoris; Thermostability

Indexed keywords

TRIACYLGLYCEROL LIPASE;

ARTICLE; CATALYSIS; CONTROLLED STUDY; DNA SHUFFLING; FUNGUS; GENE LIBRARY; NONHUMAN; PICHIA PASTORIS; POLYMERASE CHAIN REACTION; RHIZOPUS CHINENSIS; TEMPERATURE; THERMOSTABILITY;

DIRECTED MOLECULAR EVOLUTION; KINETICS; LIPASE; PICHIA; RECOMBINATION, GENETIC; RHIZOPUS; TEMPERATURE;

PICHIA PASTORIS; RHIZOPUS MICROSPORUS VAR. CHINENSIS;

EID: 84864518283     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-11-102     Document Type: Article

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