An efficient method for mutant library creation in Pichia pastoris useful in directed evolution

Biocatalysis and Biotransformation

Volumn 28, Issue 2, 2010, Pages 122-129

  Feránndez, Layla ^a   Jiao, Ning ^a   Soni, Pankaj ^a   Gumulya, Yosephine ^a   De Oliveira, Luciana Gonzaga ^a,b   Reetz, Manfred T ^a  

^a MAX PLANCK INSTITUT FÜR KOHLENFORSCHUNG   (Germany)

^b UNIVERSITY OF CAMPINAS   (Brazil)

Author keywords

Directed evolution; Expression systems; Pichia pastoris; Saturation mutagenesis

Indexed keywords

DIRECTED EVOLUTION; EFFICIENT METHOD; ENANTIOSELECTIVE; ERROR PRONE PCR; EXPRESSION SYSTEM; EXPRESSION VECTORS; GALACTOMYCES GEOTRICHUM; GENE LIBRARIES; HYDROLYTIC KINETIC RESOLUTION; LIPASE 1; MULTI-STEP PROCEDURES; MUTANT LIBRARIES; P. PASTORIS; PICHIA PASTORIS; RESTRICTION ENZYMES; SATURATION MUTAGENESIS; SIMPLIFIED METHOD; YEAST GENOME;

BACTERIOLOGY; CATALYSTS; ENZYMES; ESCHERICHIA COLI; ESTERS; MUTAGENESIS; PHOSPHORYLATION; YEAST;

GENE EXPRESSION;

ALCOHOL OXIDASE;

ARTICLE; CONTROLLED STUDY; DNA INTEGRATION; ENANTIOSELECTIVITY; ESCHERICHIA COLI; FUNGUS CULTURE; GALACTOMYCES GEOTRICHUM; GENOME; MUTAGENESIS; NONHUMAN; PICHIA PASTORIS; POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; GALACTOMYCES GEOTRICHUM; PICHIA PASTORIS;

EID: 77249098322     PISSN: 10242422     EISSN: 10292446     Source Type: Journal    
DOI: 10.3109/10242420903505834     Document Type: Article

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