Purification and characterization of an extracellular lipase from a thermophilic Rhizopus oryzae strain isolated from palm fruit

Enzyme and Microbial Technology

Volumn 26, Issue 5-6, 2000, Pages 421-430

  Hiol, Abel ^a   Jonzo, Marie D ^a   Rugani, Nathalie ^a   Druet, Danielle ^a   Sarda, Louis ^b   Comeau, Louis Claude ^a  

^a AIX MARSEILLE UNIVERSITY   (France)

^b Lab Biol Anim Univ P   (France)

Author keywords

Docosahexaenoic acid; Enzyme characterization; Extracellular lipase; Palm fruit; Palm oil acidification; Purification; Rhizopus oryzae

Indexed keywords

ALCOHOLS; AMMONIUM COMPOUNDS; BIOSYNTHESIS; CATALYST ACTIVITY; ELECTROPHORESIS; FATTY ACIDS; FUNGI; GEL PERMEATION CHROMATOGRAPHY; PH EFFECTS; PLANT CELL CULTURE; PRECIPITATION (CHEMICAL); PURIFICATION;

AMMONIUM SULFATE; DOCOSAHEXAENOIC ACID; LIPASES; LIPOLYTIC STRAIN;

ENZYMES;

DOCOSAHEXAENOIC ACID; FATTY ACID; METAL ION; PALM OIL; PROTEINASE INHIBITOR; TRIACYLGLYCEROL LIPASE;

ACIDIFICATION; ARTICLE; ENZYME ANALYSIS; ENZYME IMMOBILIZATION; ENZYME PURIFICATION; ESTERIFICATION; FRUIT; GEL FILTRATION; HYDROLYSIS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; RHIZOPUS;

RHIZOPUS ORYZAE;

EID: 0034000128     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(99)00173-8     Document Type: Article

References (39)

1. Sarda L., Desnuelle P. Action de la lipase pancréatique sur les esters en émulsion. Biochim Biophys Acta. 30:1958;513-521.
2. Desnuelle P., Sarda L., Ailhaud G. Inhibition de la lipase pancréatique par le diethyl-p-nitrophényl phosphate en émulsion. Biochim Biophys Acta. 37:1960;570-571.
3. Macrae A.R., Hammond R.C. Present and future applications of lipases. J Biotechnol Genet Eng. 3:1985;193-217.
4. Bornscheuer U.T. Lipase-catalyzed synthesis of monoglycerols. Enzyme Microb Technol. 17:1995;578-586.
5. Schmid R.D., Verger R. Interfacial enzymes with attractive applications. Angew Chem Int Ed. 37:1998;1608-1633.
6. Brady L., Brzozowski A.M., Derewenda Z.S., Dodson E., Dodson G., Tolley S., Turkenburg J.P., Christiansen L., Huge-Jensen B., Norskov L., Thim L., Menge U. A serine protease triad forms the catalytic centre of a triacylglycerol lipase. Nature. 343:1990;767-770.
7. Schrag J.D., Li Y., Wu S., Cygler M. Ser-His-Glu triad forms the catalytic site of lipase from Geotrichum candidum. Nature. 351:1991;761-764.
8. Winkler F.K., D'Arcy A., Hunziker W. Structure of human pancreatic lipase. Nature. 343:1990;771-774.
9. Cygler M., Schrag J.D. Structure as basis for understanding interfacial properties of lipases. Methods Enzymol. 284:1997;3-24.
10. Brzozowski A.M., Derewenda U., Derewenda Z.S., Dodson G.G., Lawson D.M., Turkenburg J.P., Bjorkling F., Huge-Jensen B., Patkar S.A., Thim L. A model for interfacial activation in lipases from the structure of a fungal lipase inhibitor complex. Nature. 351:1991;491-494.
11. Stead D. Microbial lipases their characteristics, role in food spoilage and industrial uses . J Dairy Res. 53:1986;481-505.
12. Eggins H.O.W., Coursey D.G. Thermophilic fungi associated with Nigerian palm produce. Nature. 203:1964;1083-1084.
13. Ogundero V.W. Degradation of Nigerian palm products by thermophilic fungi. Br Mycal Soc. 77:1981;267-271.
14. Hiol A., Jonzo M.D., Druet D., Comeau L.C. Production, purification and characterization of an extracellular lipase from Mucor hiemalis f. hiemalis. Enzyme Microb Technol. 25:1999;80-87.
15. Salleh A.B., Musani R., Basri M., Ampon W.M., Razak C.N.A. Extra- and intra-cellular lipase from a thermophilic Rhizopus oryzae and factor affecting their production. Can J Microbiol. 39:1993;978-981.
16. Schipper M.A.A. A revision of the genus Rhizopus II. The Rhizopus Stolinifer-group and Rhizopus oryzae. Stud Myco. 25:1984;1-19.
17. Essamri M., Deyris V., Comeau L. Optimization of lipase production by Rhizopus oryzae and study on the stability of lipase activity in organic solvents. J Biotechnol. 60:1998;97-101.
18. Joeger R.D., Haas M.J. Alteration of chain length selectivity of Rhizopus delemar lipase through site-directed mutagenesis. Lipids. 29:1994;377-384.
19. Samad M.Y.A., Razak C.N., Salleh A.B., Zin Wan Y., Ampon W.M., Basri M. A plate assay for primary screening of lipase activity. J Microbiol Methods. 9:1989;51-56.
20. Erlanson C. p-Nitrophenylacetate as a substrate for a carboxyl-ester hydrolase in pancreatic juice and intestinal content. Scand J Gastroent. 5:1970;333-336.
21. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the phenol folin reagent. J Biol Chem. 193:1951;265-275.
22. Laemmli U.K. Cleavage of structural proteins during the assembly of the head. Nature. 227:1970;680-685.
23. Matsudaira P. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem. 262:1970;10035-10038.
24. Rugani N., Dezan C., De la Fournière L., Cozzone P.J., Bellon B., Sarda L. Separation and characterization of the precursor and activated forms of porcine and human pancreatic colipase by reversed-phase liquid chromatography. J Chromatogr. 583:1991;246-253.
25. Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F. Colorimetric method for determination of sugars and related substances. Anal Biochem. 28:1956;350-356.
26. Jonzo M.D., Hiol A., Druet D., Comeau L.C. Application of immobilized lipase from Candida rugosa to synthesis of cholesterol oleate. J Chem Tech Biotechnol. 69:1997;463-469.
27. Rozière M. Enrichissement en acides gras polyinsaturés par voie enzymatique. Université Aix-Marseille III, Thèse de Doctorat en Chimie Biologique Appliquée, 1999.
28. Sztajer H., Lünsdorf H., Erdmann H., Menge U., Schmid R. Purification and properties of lipase from Penicillium simplicissimum. Biochim Biophys Acta. 1124:1992;253-261.
29. Haas M.J., Cichowicz D.J., Bailey D.G. Purification and characterization of an extracellular lipase from the fungus Rhizopus delemar. Lipids. 27:1992;571-576.
30. Mitsutaka K., Kugimiya W.Y., Hashimoto, Morita Y. Purification, characterization and crystallization of two types of lipase from Rhizopus niveus. Biosci Biotech Biochem. 58:1994;1007-1012.
31. Ben Salah A., Fendri K., Gargouri Y. La lipase de Rhizopus oryzae production et caractéristiques biochimiques . Rev Fr Corps Gras. 41:1994;133-137.
32. Ibrik A., Chahinian H., Rugani N., Sarda L., Comeau L. Biochemical and structural characterization of triacylglycerol lipase from Penicillium cyclopium. Lipids. 33:1998;377-384.
33. Beer H.D., McCarthy J.E.G., Bornscheuer U.T., Schmid R.D. Cloning, expression, characterization, and role of the leader sequence of a lipase from Rhizopus oryzae. Biochim Biophys Acta. 1399:1999;173-180.
34. Haas M.J., Allen J., Berka T.R. Cloning, expression and characterization of a cDNA encoding a lipase from Rhizopus delemar. Gene. 109:1991;107-113.
35. Takahashi S., Ueda M., Atomi H., Beer H.D., Bornscheuer U.T., Schmid R.D., Tanaka A. Extracellular production of active Rhizopus oryzae lipase by Saccharomyces cerevisiae. J Ferment Bioeng. 86:1998;164-168.
36. Moore S.R., McNeill G.P. Production of triglycerides enriched in long-chain n-3 polyunsaturated fatty acids from fish oil. J Am Oil Chem Soc. 73:1996;1409-1414.
37. Haraldsson G.G., Kristensson B., Sigurdardottir R., Gudmundsson G., Breivik H. The preparation of concentrates of eicosapentaenoic acid and docosahexaenoic acid by lipase-catalyzed transesterification of fish oil with ethanol. J Am Oil Chem Soc. 74:1997;1419-1424.
38. Shimada Y., Sugihara A., Nakano H., Kuramoto T., Nagao T., Gemba M., Tominaga Y. Purification of docosahexaenoic acid by selective esterification of fatty acids from tuna oil with Rhizopus delemar lipase. J Am Oil Chem Soc. 74:1997;97-101.
39. McNeill G.P., Ackman R.G., Moore S.R. Lipase-catalyzed enrichment of long-chain polyunsaturated fatty acids. J Am Oil Chem Soc. 73:1996;1403-1407.