Single-molecule tracking in living cells using single quantum dot appli-cations

Theranostics

Volumn 2, Issue 7, 2012, Pages 655-667

  Baba, Koichi ^a   Nishida, Kohji ^a  

^a OSAKA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

Author keywords

In vivo real time tracking; Membrane dynamics; Neuroscience; Nuclear pore complex; Quantum dot; Single molecule tracking

Indexed keywords

ADENOSINE TRIPHOSPHATE; G PROTEIN COUPLED RECEPTOR; GREEN FLUORESCENT PROTEIN; QUANTUM DOT; SILICON; ZINC SULFIDE;

ABSORPTION; CELL MIGRATION; CHEMICAL LABELING; CHEMICAL STRUCTURE; CONFOCAL MICROSCOPY; CYTOTOXICITY; DIFFUSION; FLUORESCENCE; INTERNALIZATION; MOLECULAR DYNAMICS; MOLECULAR IMAGING; MOLECULAR PROBE; MULTIPHOTON MICROSCOPY; NERVE CELL PLASTICITY; NEUROSCIENCE; NUCLEAR PORE COMPLEX; PARTICLE SIZE; PHOTODEGRADATION; PHOTOLUMINESCENCE; PHYSICAL CHEMISTRY; QUANTITATIVE ANALYSIS; QUANTUM YIELD; REVIEW; SENSITIVITY AND SPECIFICITY; SINGLE MOLECULE TRACKING;

EID: 84864408828     PISSN: None     EISSN: 18387640     Source Type: Journal    
DOI: 10.7150/thno.3890     Document Type: Review

References (134)

1. Li GW, Xie XS. Central dogma at the single-molecule level in living cells. Nature. 2011; 475: 308-15.
2. Joo C, Balci H, Ishitsuka Y, Buranachai C, Ha T. Advances in single-molecule fluorescence methods for molecular biology. Annu Rev Biochem. 2008; 77: 51-76.
3. Weiss S. Fluorescence spectroscopy of single biomolecules. Science. 1999; 283: 1676-83.
4. Wieser S, Schütz GJ. Tracking single molecules in the live cell plasma membrane-Do's and Don't's. Methods. 2008; 46: 131-40.
5. Saxton MJ, Jacobson K. Single-particle tracking: applications to membrane dynamics. Annu Rev Biophys Biomol Struct. 1997; 26: 373-99.
6. Shera EB, Seitzinger NK, Davis LM, Keller RA, Soper SA. Detection of single fluorescent molecules. Chem Phys Lett. 1990; 174: 553-7.
7. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC. Green fluorescent protein as a marker for gene expression. Science. 1994; 263: 802-5.
8. Shimomura O, Johnson FH, Saiga Y. Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. J Cell Comp Physiol. 1962; 59: 223-39.
9. Heim R, Prasher DC, Tsien RY. Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc Natl Acad Sci U S A. 1994; 91: 12501-4.
10. Dahan M, Levi S, Luccardini C, Rostaing P, Riveau B, Triller A. Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking. Science. 2003; 302: 442-5.
11. Pinaud F, Michalet X, Iyer G, Margeat E, Moore HP, Weiss S. Dynamic partitioning of a glycosyl-phosphatidylinositol-anchored protein in glycosphingolipid-rich microdomains imaged by single-quantum dot tracking. Traffic. 2009; 10: 691-712.
12. Jin S, Hu YX, Gu ZJ, Liu L, Wu HC. Application of quantum dots in biological imaging. J Nanomater. 2011;: 834139.
13. Smith AM, Dave S, Nie SM, True L, Gao XH. Multicolor quantum dots for molecular diagnostics of cancer. Expert Rev Mol Diagn. 2006; 6: 231-44.
14. Xing Y, Chaudry Q, Shen C, Kong KY, Zhau HE, Chung LW, et al. Bioconjugated quantum dots for multiplexed and quantitative immunohistochemistry. Nat Protoc. 2007; 2: 1152-65.
15. Yezhelyev MV, Al-Hajj A, Morris C, Marcus AI, Liu T, Lewis M, et al. In situ molecular profiling of breast cancer biomarkers with multicolor quantum dots. Adv Mater. 2007; 19: 3146-51.
16. Gao X, Cui Y, Levenson RM, Chung LW, Nie S. In vivo cancer targeting and imaging with semiconductor quantum dots. Nat Biotechnol. 2004; 22: 969-76.
17. Bharali DJ, Lucey DW, Jayakumar H, Pudavar HE, Prasad PN. Folate-receptor-mediated delivery of InP quantum dots for bioimaging using confocal and two-photon microscopy. J Am Chem Soc. 2005; 127: 11364-71.
18. Stroh M, Zimmer JP, Duda DG, Levchenko TS, Cohen KS, Brown EB, et al. Quantum dots spectrally distinguish multiple species within the tumor milieu in vivo. Nat Med. 2005; 11: 678-82.
19. Voura EB, Jaiswal JK, Mattoussi H, Simon SM. Tracking metastatic tumor cell extravasation with quantum dot nanocrystals and fluorescence emission-scanning microscopy. Nat Med. 2004; 10: 993-8.
20. Lei Y, Tang H, Yao L, Yu R, Feng M, Zou B. Applications of mesenchymal stem cells labeled with Tat peptide conjugated quantum dots to cell tracking in mouse body. Bioconjug Chem. 2008; 19: 421-7.
21. Schroeder T. Imaging stem-cell-driven regeneration in mammals. Nature. 2008; 453: 345-51.
22. Slotkin JR, Chakrabarti L, Dai HN, Carney RS, Hirata T, Bregman BS, et al. In vivo quantum dot labeling of mammalian stem and progenitor cells. Dev Dyn. 2007; 236: 3393-401.
23. De la Zerda A, Gambhir SS. Drug delivery: keeping tabs on nanocarriers. Nat Nanotechnol. 2007; 2: 745-6.
24. Medintz IL, Pons T, Delehanty JB, Susumu K, Brunel FM, Dawson PE, et al. Intracellular delivery of quantum dot-protein cargos mediated by cell penetrating peptides. Bioconjug Chem. 2008; 19: 1785-95.
25. Derfus AM, Chen AA, Min DH, Ruoslahti E, Bhatia SN. Targeted quantum dot conjugates for siRNA delivery. Bioconjug Chem. 2007; 18: 1391-6.
26. Chan WC, Maxwell DJ, Gao X, Bailey RE, Han M, Nie S. Luminescent quantum dots for multiplexed biological detection and imaging. Curr Opin Biotechnol. 2002; 13: 40-6.
27. Empedocles S, Bawendi M. Spectroscopy of single CdSe nanocrystallites. Accounts Chem Res. 1999; 32: 389-96.
28. Michalet X, Pinaud FF, Bentolila LA, Tsay JM, Doose S, Li JJ, et al. Quantum dots for live cells, in vivo imaging, and diagnostics. Science. 2005; 307: 538-44.
29. Chan WC, Nie S. Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science. 1998; 281: 2016-8.
30. Maenosono S. Core/shell quantum dots adapting to biological applica-tions (translated title from Japanese) Application of Quantum Dot in Life Science Yamamoto S, ed. 1st ed. Tokyo: CMC International Co, Ltd 2007 15-26
31. Nirmal M, Brus L. Luminescence photophysics in semiconductor nanocrystals. Acc Chem Res. 1999; 32: 407-14.
32. Alivisatos P. The use of nanocrystals in biological detection. Nat Biotechnol. 2004; 22: 47-52.
33. Murphy CJ. Optical sensing with quantum dots. Anal Chem. 2002; 74: 520A-6A.
34. Murray CB, Norris DJ, Bawendi MG. Synthesis and characterization of nearly monodisperse Cde (E = S, Se, Te) semiconductor nanocrystallites. J Am Chem Soc. 1993; 115: 8706-15.
35. Rajh T, Micic OI, Nozik AJ. Synthesis and characterization of surface-modified colloidal cadmium telluride quantum dots. J Phys Chem. 1993; 97: 11999-2003.
36. Wang Y, Chen L. Quantum dots, lighting up the research and development of nanomedicine. Nanomedicine. 2011; 7: 385-402.
37. Law WC, Yong KT, Roy I, Ding H, Hu R, Zhao W, et al. Aqueous-phase synthesis of highly luminescent CdTe/ZnTe core/shell quantum dots optimized for targeted bioimaging. Small. 2009; 5: 1302-10.
38. Bruchez Jr M, Moronne M, Gin P, Weiss S, Alivisatos AP. Semiconductor nanocrystals as fluorescent biological labels. Science. 1998; 281: 2013-6.
39. Biju V, Itoh T, Ishikawa M. Delivering quantum dots to cells: bioconjugated quantum dots for targeted and nonspecific extracellular and intracellular imaging. Chem Soc Rev. 2010; 39: 3031-56.
40. Erathodiyil N, Ying JY. Functionalization of inorganic nanoparticles for bioimaging applications. Acc Chem Res. 2011; 44: 925-35.
41. Medintz IL, Uyeda HT, Goldman ER, Mattoussi H. Quantum dot bioconjugates for imaging, labelling and sensing. Nat Mater. 2005; 4: 435-46.
42. Biju V, Itoh T, Anas A, Sujith A, Ishikawa M. Semiconductor quantum dots and metal nanoparticles: syntheses, optical properties, and biological applications. Anal Bioanal Chem. 2008; 391: 2469-95.
43. Oh EJ, Park K, Kim KS, Kim J, Yang JA, Kong JH, et al. Target specific and long-acting delivery of protein, peptide, and nucleotide therapeutics using hyaluronic acid derivatives. J Control Release. 2010; 141: 2-12.
44. Fore S, Laurence TA, Hollars CW, Huser T. Counting constituents in molecular complexes by fluorescence photon antibunching. Ieee J Sel Top Quant. 2007; 13: 996-1005.
45. McHale K, Berglund AJ, Mabuchi H. Quantum dot photon statistics measured by three-dimensional particle tracking. Nano Lett. 2007; 7: 3535-39.
46. Lounis B, Bechtel HA, Gerion D, Alivisatos P, Moerner WE. Photon antibunching in single CdSe/ZnS quantum dot fuorescence. Chem Phys Lett. 2000; 329: 399-404.
47. Zwiller V, Blom H, Jonsson P, Panev N, Jeppesen S, Tsegaye T, et al. Single quantum dots emit single photons at a time: antibunching experiments. Appl Phys Lett. 2001; 78: 2476-8.
48. Nathan P. Wells NP, Lessard GA, Werner JH. Confocal, three-dimensional tracking of individual quantum dots in high-background environments. Anal Chem. 2008; 80: 9830-34.
49. Yuan CT, Yu P, Ko HC, Huang J, Tang J. Antibunching single-photon emission and blinking suppression of CdSe/ZnS quantum dots. ACS NANO. 2009; 3: 3051-56.
50. Wissert MD, Rudat B, Lemmer U, Eisler HJ. Quantum dots as single-photon sources: Antibunching via two-photon excitation. Phys Rev B. 2011; 83: 113304.
51. De Lange F, Cambi A, Huijbens R, De Bakker B, Rensen W, Garcia-Parajo M, et al. Cell biology beyond the diffraction limit: near-field scanning optical microscopy. J Cell Sci. 2001; 114: 4153-60.
52. Sako Y, Yanagida T. Single-molecule visualization in cell biology. Nat Rev Mol Cell Bio. 2003;: SS1-SS5.
53. Ho YP, Leong KW. Quantum dot-based theranostics. Nanoscale. 2010; 2: 60-8.
54. Cang H, Xu CS, Montiel D, Yang H. Guiding a confocal microscope by single fluorescent nanoparticles. Opt Lett. 2007; 32: 2729-31.
55. Holtzer L, Meckel T, Schmidt T. Nanometric three-dimensional tracking of individual quantum dots in cells. Appl Phys Lett. 2007; 90: 053902.
56. Lessard GA, Goodwin PM, Werner JH. Three-dimensional tracking of individual quantum dots. Appl Phys Lett. 2007; 91: 224106.
57. Ram S, Prabhat P, Chao J, Ward ES, Ober RJ. High accuracy 3D quantum dot tracking with multifocal plane microscopy for the study of fast intracellular dynamics in live cells. Biophys J. 2008; 95: 6025-43.
58. Thompson MA, Lew MD, Badieirostami M, Moerner WE. Localizing and tracking single nanoscale emitters in three dimensions with high spatiotemporal resolution using a double-helix point spread function. Nano Lett. 2010; 10: 211-8.
59. Toprak E, Balci H, Blehm BH, Selvin PR. Three-dimensional particle tracking via bifocal imaging. Nano Lett. 2007; 7: 2043-5.
60. Wells NP, Lessard GA, Werner JH. Confocal, three-dimensional tracking of individual quantum dots in high-background environments. Anal Chem. 2008; 80: 9830-4.
61. Wells NP, Lessard GA, Goodwin PM, Phipps ME, Cutler PJ, Lidke DS, et al. Time-resolved three-dimensional molecular tracking in live cells. Nano Lett. 2010; 10: 4732-7.
62. Qian H, Sheetz MP, Elson EL. Single particle tracking. Analysis of diffusion and flow in two-dimensional systems. Biophys J. 1991; 60: 910-21.
63. Pinaud F, Clarke S, Sittner A, Dahan M. Probing cellular events, one quantum dot at a time. Nat Methods. 2010; 7: 275-85.
64. Michalet X. Mean square displacement analysis of single-particle trajectories with localization error: brownian motion in an isotropic medium. Phys Rev E Stat Nonlin Soft Matter Phys. 2010; 82: 041914.
65. Durisic N, Bachir AI, Kolin DL, Hebert B, Lagerholm BC, Grutter P, et al. Detection and correction of blinking bias in image correlation transport measurements of quantum dot tagged macromolecules. Biophys J. 2007; 93: 1338-46.
66. Huet S, Karatekin E, Tran VS, Fanget I, Cribier S, Henry JP. Analysis of transient behavior in complex trajectories: application to secretory vesicle dynamics. Biophys J. 2006; 91: 3542-59.
67. Pierobon P, Cappello G. Quantum dots to tail single bio-molecules inside living cells. Adv Drug Deliver Rev. 2012; 64: 167-78.
68. Fujiwara T, Ritchie K, Murakoshi H, Jacobson K, Kusumi A. Phospholipids undergo hop diffusion in compartmentalized cell membrane. J Cell Biol. 2002; 157: 1071-81.
69. Andrews NL, Lidke KA, Pfeiffer JR, Burns AR, Wilson BS, Oliver JM, et al. Actin restricts Fc epsilon RI diffusion and facilitates antigen-induced receptor immobilization. Nat Cell Biol. 2008; 10: 955-63.
70. Elf J, Li GW, Xie XS. Probing transcription factor dynamics at the single-molecule level in a living cell. Science. 2007; 316: 1191-4.
71. Gelles J, Schnapp BJ, Sheetz MP. Tracking kinesin-driven movements with nanometre-scale precision. Nature. 1988; 331: 450-3.
72. Yildiz A, Forkey JN, McKinney SA, Ha T, Goldman YE, Selvin PR. Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization. Science. 2003; 300: 2061-5.
73. Derfus AM, Chan WCW, Bhatia SN. Intracellular delivery of quantum dots for live cell labeling and organelle tracking. Adv Mater. 2004; 16: 961-6.
74. Dubertret B, Skourides P, Norris DJ, Noireaux V, Brivanlou AH, Libchaber A. In vivo imaging of quantum dots encapsulated in phospholipid micelles. Science. 2002; 298: 1759-62.
75. Keren K, Yam PT, Kinkhabwala A, Mogilner A, Theriot JA. Intracellular fluid flow in rapidly moving cells. Nat Cell Biol. 2009; 11: 1219-24.
76. Ishihama Y, Funatsu T. Single molecule tracking of quantum dot-labeled mRNAs in a cell nucleus. Biochem Biophys Res Commun. 2009; 381: 33-8.
77. Singer SJ, Nicolson GL. The fluid mosaic model of the structure of cell membranes. Science. 1972; 175: 720-31.
78. Hopkins AL, Groom CR. The druggable genome. Nat Rev Drug Discov. 2002; 1: 727-30.
79. Fichter KM, Flajolet M, Greengard P, Vu TQ. Kinetics of G-protein-coupled receptor endosomal trafficking pathways revealed by single quantum dots. P Natl Acad Sci USA. 2010; 107: 18658-63.
80. Hussl S, Boehm S. Functions of neuronal P2Y receptors. Pflugers Arch. 2006; 452: 538-51.
81. Surprenant A, North RA. Signaling at purinergic P2X receptors. Annu Rev Physiol. 2009; 71: 333-59.
82. Khakh BS. Molecular physiology of P2X receptors and ATP signalling at synapses. Nat Rev Neurosci. 2001; 2: 165-74.
83. Jiang S, Liu A, Duan H, Soo J, Chen P. Labeling and tracking P2 purinergic receptors in living cells using ATP-conjugated quantum dots. Adv Funct Mater. 2011; 21: 2776-80.
84. Triller A, Choquet D. New concepts in synaptic biology derived from single-molecule imaging. Neuron. 2008; 59: 359-74.
85. Groc L, Heine M, Cousins SL, Stephenson FA, Lounis B, Cognet L, et al. NMDA receptor surface mobility depends on NR2A-2B subunits. Proc Natl Acad Sci U S A. 2006; 103: 18769-74.
86. Heine M, Groc L, Frischknecht R, Beique JC, Lounis B, Rumbaugh G, et al. Surface mobility of postsynaptic AMPARs tunes synaptic transmission. Science. 2008; 320: 201-5.
87. Groc L, Lafourcade M, Heine M, Renner M, Racine V, Sibarita JB, et al. Surface trafficking of neurotransmitter receptor: comparison between single-molecule/quantum dot strategies. J Neurosci. 2007; 27: 12433-7.
88. Ehrensperger MV, Hanus C, Vannier C, Triller A, Dahan M. Multiple association states between glycine receptors and gephyrin identified by SPT analysis. Biophys J. 2007; 92: 3706-18.
89. Levi S, Schweizer C, Bannai H, Pascual O, Charrier C, Triller A. Homeostatic regulation of synaptic GlyR numbers driven by lateral diffusion. Neuron. 2008; 59: 261-73.
90. Mikasova L, Groc L, Choquet D, Manzoni OJ. Altered surface trafficking of presynaptic cannabinoid type 1 receptor in and out synaptic terminals parallels receptor desensitization. Proc Natl Acad Sci U S A. 2008; 105: 18596-601.
91. Geng L, Qian YK, Madhavan R, Peng HB. Transmembrane mechanisms in the assembly of the postsynaptic apparatus at the neuromuscular junction. Chem Biol Interact. 2008; 175: 108-12.
92. Zarbin MA, Montemagno C, Leary JF, Ritch R. Nanomedicine in ophthalmology: the new frontier. Am J Ophthalmol. 2010; 150: 144-62.
93. Zarbin MA. Current concepts in the pathogenesis of age-related macular degeneration. Arch Ophthalmol. 2004; 122: 598-614.
94. Takeda A, Baffi JZ, Kleinman ME, Cho WG, Nozaki M, Yamada K, et al. CCR3 is a target for age-related macular degeneration diagnosis and therapy. Nature. 2009; 460: 225-30.
95. Lowe AR, Siegel JJ, Kalab P, Siu M, Weis K, Liphardt JT. Selectivity mechanism of the nuclear pore complex characterized by single cargo tracking. Nature. 2010; 467: 600-3.
96. Tada H, Higuchi H, Wanatabe TM, Ohuchi N. In vivo real-time tracking of single quantum dots conjugated with monoclonal anti-HER2 antibody in tumors of mice. Cancer Research. 2007; 67: 1138-44.
97. Bottrill M, Green M. Some aspects of quantum dot toxicity. Chem Commun. 2011; 47: 7039-50.
98. Hoshino A, Hanada S, Yamamoto K. Toxicity of nanocrystal quantum dots: the relevance of surface modifications. Arch Toxicol. 2011; 85: 707-20.
99. Hardman R. A toxicologic review of quantum dots: toxicity depends on physicochemical and environmental factors. Environ Health Perspect. 2006; 114: 165-72.
100. Rzigalinski BA, Strobl JS. Cadmium-containing nanoparticles: perspectives on pharmacology and toxicology of quantum dots. Toxicol Appl Pharmacol. 2009; 238: 280-8.
101. Fowler BA. Monitoring of human populations for early markers of cadmium toxicity: a review. Toxicol Appl Pharmacol. 2009; 238: 294-300.
102. Derfus AM, Chan WCW, Bhatia SN. Probing the cytotoxicity of semiconductor quantum dots. Nano Lett. 2004; 4: 11-8.
103. Lin CH, Chang LW, Chang H, Yang MH, Yang CS, Lai WH, et al. The chemical fate of the Cd/Se/Te-based quantum dot 705 in the biological system: toxicity implications. Nanotechnology. 2009; 20: 215101.
104. Sanvicens N, Marco MP. Multifunctional nanoparticles - properties and prospects for their use in human medicine. Trends in Biotechnology. 2008; 26: 425-33.
105. Su Y, He Y, Lu H, Sai L, Li Q, Li W, et al. The cytotoxicity of cadmium based, aqueous phase - synthesized, quantum dots and its modulation by surface coating. Biomaterials. 2009; 30: 19-25.
106. Selvan ST, Patra PK, Ang CY, Ying JY. Synthesis of silica-coated semiconductor and magnetic quantum dots and their use in the imaging of live cells. Angew Chem Int Ed. 2007; 46: 2448-52.
107. Tamang S, Beaune G, Texier I, Reiss P. Aqueous phase transfer of InP/ZnS nanocrystals conserving fluorescence and high colloidal stability. ACS Nano. 2011; 5: 9392-402.
108. Yong KT, Ding H, Roy I, Law WC, Bergey EJ, Maitra A, et al. Imaging pancreatic cancer using bioconjugated InP quantum dots. ACS Nano. 2009; 3: 502-10.
109. Yong KT, Roy I, Hu R, Ding H, Cai H, Zhu J, et al. Synthesis of ternary CuInS(2)/ZnS quantum dot bioconjugates and their applications for targeted cancer bioimaging. Integr Biol. 2010; 2: 121-9.
110. Banin U, Bruchez M, Alivisatos AP, Ha T, Weiss S, Chemla DS. Evidence for a thermal contribution to emission intermittency in single CdSe/CdS core/shell nanocrystals. J Chem Phys. 1999; 110: 1195-201.
111. Basche T. Fluorescence intensity fluctuations of single atoms, molecules and nanoparticles. J Lumin. 1998; 76-7: 263-9.
112. Neuhauser RG, Shimizu KT, Woo WK, Empedocles SA, Bawendi MG. Correlation between fluorescence intermittency and spectral diffusion in single semiconductor quantum dots. Phys Rev Lett. 2000; 85: 3301-4.
113. Sugisaki M, Ren HW, Nishi K, Masumoto Y. Fluorescence intermittency in self-assembled InP quantum dots. Phys Rev Lett. 2001; 86: 4883-6.
114. Nirmal M, Dabbousi BO, Bawendi MG, Macklin JJ, Trautman JK, Harris TD, et al. Fluorescence intermittency in single cadmium selenide nanocrystals. Nature. 1996; 383: 802-4.
115. Fomenko V, Nesbitt DJ. Solution control of radiative and nonradiative lifetimes: a novel contribution to quantum dot blinking suppression. Nano Lett. 2008; 8: 287-93.
116. Hohng S, Ha T. Near-complete suppression of quantum dot blinking in ambient conditions. J Am Chem Soc. 2004; 126: 1324-5.
117. Chen Y, Vela J, Htoon H, Casson JL, Werder DJ, Bussian DA, et al. "Giant" multishell CdSe nanocrystal quantum dots with suppressed blinking. J Am Chem Soc. 2008; 130: 5026-7.
118. Mahler B, Spinicelli P, Buil S, Quelin X, Hermier JP, Dubertret B. Towards non-blinking colloidal quantum dots. Nat Mater. 2008; 7: 659-64.
119. Vela J, Htoon H, Chen Y, Park YS, Ghosh Y, Goodwin PM, et al. Effect of shell thickness and composition on blinking suppression and the blinking mechanism in 'giant' CdSe/CdS nanocrystal quantum dots. J Biophotonics. 2010; 3: 706-17.
120. Wang X, Ren X, Kahen K, Hahn MA, Rajeswaran M, Maccagnano-Zacher S, et al. Non-blinking semiconductor nanocrystals. Nature. 2009; 459: 686-9.
121. Cao YC. Impurities enhance semiconductor nanocrystal performance. Science. 2011; 332: 48-9.
122. Mocatta D, Cohen G, Schattner J, Millo O, Rabani E, Banin U. Heavily doped semiconductor nanocrystal quantum dots. Science. 2011; 332: 77-81.
123. Choi J, Wang NS, Reipa V. Conjugation of the photoluminescent silicon nanoparticles to streptavidin. Bioconjug Chem. 2008; 19: 680-5.
124. Erogbogbo F, Tien CA, Chang CW, Yong KT, Law WC, Ding H, et al. Bioconjugation of luminescent silicon quantum dots for selective uptake by cancer cells. Bioconjug Chem. 2011; 22: 1081-8.
125. Erogbogbo F, Yong KT, Roy I, Xu G, Prasad PN, Swihart MT. Biocompatible luminescent silicon quantum dots for imaging of cancer cells. ACS Nano. 2008; 2: 873-8.
126. Shiohara A, Prabakar S, Faramus A, Hsu CY, Lai PS, Northcote PT, et al. Sized controlled synthesis, purification, and cell studies with silicon quantum dots. Nanoscale. 2011; 3: 3364-70.
127. He GS, Zheng Q, Yong KT, Erogbogbo F, Swihart MT, Prasad PN. Two- and three-photon absorption and frequency upconverted emission of silicon quantum dots. Nano Lett. 2008; 8: 2688-92.
128. Baba K, Kasai H, Masuhara A, Oikawa H, Nakanishi H. Organic solvent-free fluorescence confocal imaging of living cells using pure nanocrystal forms of fluorescent dyes. Jpn J Appl Phys. 2009; 48: 117002.
129. Baba K, Pudavar HE, Roy I, Ohulchanskyy TY, Chen Y, Pandey RK, et al. New method for delivering a hydrophobic drug for photodynamic therapy using pure nanocrystal form of the drug. Mol Pharm. 2007; 4: 289-97.
130. Kasai H, Nalwa HS, Okada S, Oikawa H, Nakanishi H. Fabrication and spectroscopic characterization of organic nanocrystals Handbook of Nanostructured Materials and Nanotechnology, Organics, Polymers, and Biological Materials Nalwa HS, ed. 1st ed. Sandego: Academic Press 1999 433-73
131. Masuo S, Masuhara A, Akashi T, Muranushi M, Machida S, Kasai H, et al. Photon antibunching in the emission from a single organic dye nanocrystal. Jpn J Appl Phys. 2007; 46: L268-L270.
132. Aharonovich I, Castelletto S, Simpson DA, Su CH, Greentree AD, Prawer S. Diamond-based single-photon emitters. Rep Prog in Phys. 2011; 74: 076501.
133. Faklaris O, Joshi V, Irinopoulou T, Tauc P, Sennour M, Girard H, et al. Photoluminescent diamond nanoparticles for cell labeling: study of the uptake mechanism in mammalian cells. ACS Nano. 2009; 3: 3955-62.
134. Mochalin VN, Gogotsi Y. Wet chemistry route to hydrophobic blue fluorescent nanodiamond. J Am Chem Soc. 2009; 131: 4594-5