Myosin V walks hand-over-hand: Single fluorophore imaging with 1.5-nm localization

Science

Volumn 300, Issue 5628, 2003, Pages 2061-2065

  Yildiz, Ahmet ^a   Forkey, Joseph N ^c   McKinney, Sean A ^a,b   Ha, Taekjip ^a,b   Goldman, Yale E ^c   Selvin, Paul R ^a,b  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b University of Illinois at Urbana Champaign   (United States)

^c UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINETRIPHOSPHATE; HYDROLYSIS; MOLECULES;

FLUOROPHORE IMAGING;

IMAGING TECHNIQUES;

ACTIN; ADENOSINE TRIPHOSPHATE; DYE; MYOSIN V;

BIOLOGY; CHEMISTRY; IMAGING METHOD;

ARTICLE; CALCULATION; HYDROLYSIS; IMAGING SYSTEM; MOLECULAR DYNAMICS; MOLECULAR MODEL; MOTION; PARTICLE SIZE; PRIORITY JOURNAL; PROTEIN LOCALIZATION;

ACTINS; ADENOSINE TRIPHOSPHATE; BINDING SITES; CALMODULIN; CARBOCYANINES; CATALYTIC DOMAIN; DNA; FLUORESCENCE; FLUORESCENT DYES; KINETICS; MATHEMATICS; MICROFILAMENTS; MICROSCOPY, FLUORESCENCE; MODELS, BIOLOGICAL; MOLECULAR MOTOR PROTEINS; MYOSIN LIGHT CHAINS; MYOSIN TYPE V; PROTEIN STRUCTURE, TERTIARY; RHODAMINES;

EID: 0037832404     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1084398     Document Type: Article

References (33)

1. A. D. Mehta et al., Nature 400, 590 (1999).
2. C. Veigel, F. Wang, M. L. Bartoo, J. R. Sellers, J. E. Molloy, Nature Cell Biol. 4, 59 (2002).
3. A. Mehta, J. Cell Sci. 114, 1981 (2001).
4. S. L. Reck-Peterson, D. W. Provance Jr., M. S. Mooseker, J. A. Mercer, Biochim. Biophys. Acta 1496, 36 (2000).
5. T. J. Purcell, C. Morris, J. A. Spudich, H. L. Sweeney, Proc. Natl. Acad. Sci. U.S.A. 99, 14159 (2002).
6. J. N. Forkey, M. E. Quinlan, M. T. Shaw, J. E. Corrie, Y. E. Goldman, Nature 422, 399 (2003).
7. E. M. De La Cruz, A. L. Wells, S. S. Rosenfeld, E. M. Ostap, H. L. Sweeney, Proc. Natl. Acad. Sci. U.S.A. 96, 13726 (1999).
8. W. Hua, J. Chung, J. Gelles, Science 295, 844 (2002).
9. M. Y. Ali et al., Nature Struct. Biol. 9, 464 (2002).
10. R. S. Rock et al., Proc. Natl. Acad. Sci. U.S.A. 98, 13655 (2001).
11. D. Axelrod, Methods Cell Biol. 30, 245 (1989).
12. T. Funatsu, Y. Harada, M. Tokunaga, K. Saito, T. Yanagida, Nature 374, 555 (1995).
13. M. Tokunaga, K. Kitamura, K. Saito, A. H. Iwane, T. Yanagida, Biochem. Biophys. Res. Commun. 235, 47 (1997).
14. Materials and Methods are available as supporting online material on Science Online.
15. T. Schmidt, G. J. Schütz, W. Baumgartner, H. J. Gruber, H. Schindler, Proc. Natl. Acad. Sci. U.S.A. 93, 2926 (1996).
16. U. Kubitscheck, O. Kückmann, T. Kues, R. Peters, Biophys. J. 78, 2170 (2000).
17. T. D. Lacoste et al., Proc. Natl. Acad. Sci. U.S.A. 97, 9461 (2000).
18. R. E. Thompson, D. R. Larson, W. W. Webb, Biophys. J. 82, 2775 (2002).
19. J. Gelles, B. J. Schnapp, M. P. Sheetz, Nature 331, 450 (1988).
20. N. Bobroff, Rev. Sci. Inst. 57, 1152 (1986).
21. M. K. Cheezum, W. F. Walker, W. H. Guilford, Biophys. J. 81, 2378 (2001).
22. Y. Harada, K. Sakurada, T. Aoki, D. D. Thomas, T. Yanagida, J. Mol. Biol. 216, 49 (1990).
23. K. Adachi et al., Proc. Natl. Acad. Sci. U.S.A. 97, 7243 (2000).
24. Y. Sambongi et al., Science 286, 1722 (1999).
25. J. E. T. Corrie et al., Nature 400, 425 (1999).
26. T. Sakamoto, I. Amitani, E. Yokota, T. Ando, Biochem. Biophys. Res. Commun. 272, 586 (2000).
27. A. P. Bartko, R. M. Dickson, J. Phys. Chem. B 103, 3053 (1999).
28. 2 can differ by a factor of 2 or more. Hence, we cannot rule out asymmetry in the rates of the two heads.
29. M. L. Walker et al., Nature 405, 804 (2000).
30. M. Rief et al., Proc. Natl. Acad. Sci. U.S.A. 97, 9482 (2000).
31. S. Burgess et al., J. Cell Biol. 159, 983 (2002).
32. J. A. Spudich, Nature Rev. Mol. Cell Biol. 2, 387 (2001).
33. Supported by NIH grants AR44420 (to P.R.S.), GM65367 (to T.H.), and AR26846 (to Y.E.G.); NSF grants DBI-02-15869 (to P.R.S. and T.H.) and 9984841 (to P.R.S.); the Carver Trust Foundation (to P.R.S.); and the DOE, Division of MateriaLs Sciences (under award no. DEFG02-91ER45439), through the Frederick Seitz MateriaLs Research Laboratory at the University of Illinois at Urbana-Champaign (to P.R.S.). S.A.M. was supported by a National Research Service Award in Molecular Biophysics (through NIH training grant PHS 5 T32 GM08276). We thank J. E. T. Corrie for gift of bis-rhodamine, E. Gratton for initial suggestions and discussions, B. C. Stevens for computer programming assistance, and I. Rasnik for fluorescence imaging assistance.