Single-molecule visualization in cell biology

Nature Reviews Molecular Cell Biology

Volumn 4, Issue SUPPL., 2003, Pages

  Sako, Yasushi ^a,b   Yanagida, Toshio ^a,b  

^a OSAKA UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CELL METABOLISM; CHEMICAL REACTION KINETICS; CYTOLOGY; IMAGE QUALITY; MOLECULAR BIOLOGY; MOLECULAR DYNAMICS; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVIEW; SINGLE MOLECULE DETECTION TECHNIQUE; SINGLE MOLECULE VISUALIZATION; SPATIAL RESOLUTION; TEMPORAL RESOLUTION; ANIMAL; CELL FUNCTION; EUKARYOTIC CELL; FLUORESCENCE MICROSCOPY; HUMAN; METABOLISM;

FLUORESCENT DYE; PHOTOPROTEIN; PROTEIN;

ANIMALS; CELL PHYSIOLOGY; EUKARYOTIC CELLS; FLUORESCENT DYES; HUMANS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; PROTEINS;

EID: 0041837560     PISSN: 14710072     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (44)

1. Weiss, S. Measuring conformational dynamics of biomolecules by single molecule fluorescence spectroscopy. Nature Struct. Biol. 7, 724-729 (2000).
2. Ishijima, A. & Yanagida, T. Single molecule nanobiosdence. Trends Biochem. Sci. 26, 438-444 (2001).
3. Xie, S. Single-molecule approach to enzymology. Single Mol. 2, 229-236 (2001).
4. Hinterdorfer, P., Schütz, G., Kienberger, F. & Schindler, H. Detection and characterization of single biomolecules at surfaces. J. Biotechnol. 82, 25-35 (2001).
5. Sako, Y. & Uyemura, T. Total internal reflection fluorescence microscopy for single-molecule imaging in living cells. Cell Struct. Funct. 27, 205-213 (2002).
6. Funatsu, T., Harada, Y., Tokunaga, M., Saito, K. & Yanagida, T. Imaging of single fluorescent molecules and individual ATP turnover by single myosin molecules in aqueous solution. Nature 374, 555-559 (1995).
7. Sase, I., Miyata, H., John, C. E. T., James, C. S. & Kinoshita, K. Jr. Real time imaging of single fluorophores on moving actin with an epifluorescence microscope. Biophys. J. 69, 323-328 (1995).
8. Sako, Y., Minoguchi, S. & Yanagida, T. Single molecule imaging of EGFR signal transduction on the living cell surface. Nature Cell Biol. 2, 168-172 (2000).
9. Schütz, G. J., Gerald, K., Pastuchenko, V. P. & Schindler, H. Properties of lipid microdomains in a muscle cell membrane visualized by single molecule microscopy. EMBO J. 19, 892-901 (2000).
10. Schütz, G. J. et al. 3D imaging of individual ion channels in live cells at 40 nm resolution. Single Mol. 1, 25-31 (2000).
11. Sako, Y., Hibino, K., Miyauchi, T., Miyamoto, Y., Ueda, M. & Yanagida, T. Single-molecule imaging of signaling molecules in living cells. Single Mol. 1, 151-155 (2000).
12. Iino, R., Koyama, I. & Kusumi, A. Single molecule imaging of green fluorescent proteins in living cells: E-cadherin forms oligomers on the free cell surface. Biophys. J. 80, 2667-2677 (2001).
13. Seisenberger, G., Ried, M. U., Müning, T. E. H., Hallek,M. & Bräuchle, C. Real-time single-molecule imaging of the infection pathway of an adeno-associated virus. Science 294, 1929-1932 (2002).
14. Watanabe, N. & Mitchison, T. J. Single-molecule speckle analysis of actin filament turnover in lamellipodia. Science 295, 1083-1086 (2002).
15. Axelrod, D., Burghardt, T. P. & Thompson, N. L. Total internal reflection fluorescence. Ann. Rev. Biophys. Bioeng. 13, 247-268 (1964).
16. Tokunaga, M., Kitamura, K., Saito, K., Iwane, A. H. & Yanagida, T. Single molecule imaging of fluorophores and enzymatic reactions achieved by objective-type total internal reflection fluorescence microscopy. Biochem. Biophys. Res. Commun. 235, 47-53 (1997).
17. Tadakuma, H., Yamaguchi, J., Ishihama, Y. & Funatsu, T. Imaging of single fluorescent molecules using video-rate confocal microscopy. Biochem. Biophys. Res. Commun. 287, 323-327 (2001).
18. de Lange, F. et al. Cell biology beyond the diffraction limit: near-field scanning optical microscopy. J. Cell. Sci. 114, 4153-4160 (2001).
19. Elson, E. L. & Magde, D. Fluorescence correlation spectroscopy, I. Conceptual basis and theory. Biopolymers 13, 1-27 (1974).
20. Ueda, M., Sako, Y., Tanaka, T., Devreotes, P. & Yanagida, T. Single-molecule analysis of chemotactic signaling in Dictyostelium cells. Science 294, 864-867 (2001).
21. Goulian, M. & Simon, S. M. Tracking single proteins within cells. Biophys, J. 79, 2188-2198 (2000).
22. Kues, T., Dickmanns, A., Lührmann, R., Peters, R. & Kubitscheck, U. High intranudear mobility and dynamic clustering of the splicing factor U1 snRNP observed by single particle tracking. Proc. Natl Acad. Sci. USA 98, 12021-12026 (2001).
23. 2+ channels in live cells. Biophys. J. 81, 2639-2646 (2001).
24. Parent, C. A. & Devreotes, P. N. A cell's sense of direction. Science 284, 765-770 (1999).
25. Schlessinger, J. Cell signaling by receptor tyrosine kinases. Cell 103, 211-225 (2000).
26. Kano, F., Sako, Y., Tagaya, M., Yanagida, T. & Murata, M. Reconstitution of brefeldin A-induced Golgi-tubulation and fusion with the endoplasmic reticulum in semi-intact Chinese hamster ovary cells. Mol. Biol. Cell 11, 3073-3087 (2000).
27. Ashkin, A., Dziedzic, J. M., Bjorkholm, J. E. & Chu. S. Observation of a single-beam gradient force optical trap for dielectdc particles. Opt. Lett. 11, 288-290 (1986).
28. Svoboda, K., Schumidt, C. F., Schnapp, B. J. & Block, S. M. Direct observation of kinesin stepping by optical trapping interferometry. Nature 365, 721-727 (1993).
29. Howard, J. Molecular motors: structural adaptations to cellular functions. Nature 389, 561-567 (1997).
30. Yanagida, T., Kitamura, K., Tanaka, H., Iwane, A. H. & Esaki, S. Single molecule analysis of the actomyosin motor. Curr. Opin. Cell Biol. 12, 20-25 (2000).
31. Spudich, J. A. The myosin swinging cross-bridge model. Nature Rev. Mol. Cell Biol. 2, 387-392 (2001).
32. Ishijima, A., Kojima, H., Funatsu, T., Tokunaga, M., Higuchi, H. & Yangaida, T. Simultaneous measurement of chemical and mechanical reactions. Cell 92, 161-171 (1998).
33. Spudich, J. A. How molecular motors work. Nature 372, 515-518 (1994).
34. Mehta, A. D. et al. Myosin-V is a processive actin-based motor. Nature 400, 590-593 (1999).
35. Sakamoto, T., Amitani, I., Yokota, E. & Ando, T. Direct observation of processive movement by individual myosin V molecules. Biochem. Biophys. Res. Comm. 272, 586-590 (2000).
36. Forkey, J. N., Quinlan, M. E., Shaw, M. A., Corrie, J. E. T. & Goldman, Y. E. Three-dimensional structural dynamics of myosin V by single-molecule fluorescence polarization. Nature 422, 399-404 (2003).
37. Tanaka, H. et al. The motor domain determines the large step of myosin-V Nature 415, 192-195 (2002).
38. Rock, R. S. et al. Myosin VI is a processive motor with a large step size. Proc. Natl Acad Sci. USA 98, 13655-13659 (2001).
39. Nishikawa, S. et al. Class VI myosin moves processively along actin filament backwards with large steps. Biochem. Biophys. Res. Commun. 290, 311-317 (2002).
40. Kitamura, K., Tokunaga, M., Iwane, A. H. & Yanagida, T. A single myosin head moves along an actin filament with regular steps of -5.3 nanometres. Nature 397, 129-134 (1999).
41. Sakmann, B. & Neher, E. (eds) Single-Channel Recording (Plenum Press, New York, 1995).
42. Sonnleitner, A., Mannuzzu, L., Terakawa, S. & Isacoff, E. Y. Structural rearrangements in single ion channels detected optically in living cells. Proc. Natl Acad. Sci. USA 99, 12759-12764 (2002).
43. Ide, T., Takeuchi, Y. & Yanagida, T. Development of an experimental apparatus for simultaneous observation of optical and electrical signals from single ion channels. Single Mol. 3, 33-42 (2002).
44. Edidin, M., Kuo, S. C. & Sheetz, M. P. Lateral movements of membrane glycoproteins restricted by dynamic cytoplasmic barriers. Science 254, 1379-1382 (1991).