Probing transcription factor dynamics at the single-molecule level in a living cell

Science

Volumn 316, Issue 5828, 2007, Pages 1191-1194

  Elf, Johan ^a   Li, Gene Wei ^b   Xie, X Sunney ^a  

^a HARVARD UNIVERSITY   (United States)

^b HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

REPRESSOR PROTEIN; TRANSCRIPTION FACTOR;

COLIFORM BACTERIUM; DNA; GENE EXPRESSION; GENETIC ANALYSIS; METABOLISM; PROTEIN; RESIDENCE TIME;

ARTICLE; ESCHERICHIA COLI; FLUORESCENCE; LACTOSE OPERON; MOLECULE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN TRANSPORT; REPRESSOR GENE;

BACTERIAL PROTEINS; DIFFUSION; DNA, BACTERIAL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; KINETICS; LAC OPERON; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; OPERATOR REGIONS (GENETICS); PROTEIN BINDING; REPRESSOR PROTEINS; SIGNAL TRANSDUCTION;

ESCHERICHIA COLI;

EID: 34249932435     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1141967     Document Type: Article

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30. We thank P. Blainey, P. Sims, P. Choi, O. Berg, and M. Lovmar for helpful discussions. We are grateful to P. Blainey, P. Sims, and J. Heam for protein purification, B. English for help with FCS, and J. Yu for making pVS155 and PVS167. The plasmids pk03, pKD30, and Venus gene were contributed by G. Church, B. Wanner, and A. Miyawaki, respectively. This work was supported by NW Director's Pioneer Award Program, the Knut and Alice Wallenberg Foundation (J.E), and N5F Graduate Research Fellowship (G.-W.L).