Diffusion Dynamics of Glycine Receptors Revealed by Single-Quantum Dot Tracking

Science

Volumn 302, Issue 5644, 2003, Pages 442-445

  Dahan, Maxime ^a   Lévi, Sabine ^b   Luccardini, Camilla ^a   Rostaing, Philippe ^b   Riveau, Béatrice ^b   Triller, Antoine ^b  

^a LABORATOIRE KASTLER BROSSEL   (France)

^b ECOLE NORMALE SUPÉRIEURE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CELL MEMBRANES; DIFFUSION; FLUORESCENCE; MEDICAL IMAGING; NEUROLOGY; SEMICONDUCTOR QUANTUM DOTS;

SYNAPSES;

AMINO ACIDS;

FLUORESCENT DYE; GLYCINE RECEPTOR;

ARTICLE; DIFFUSION; ELECTRON MICROSCOPY; NERVE CELL MEMBRANE; PRIORITY JOURNAL; PROTEIN LOCALIZATION; QUANTUM MECHANICS; SEMICONDUCTOR; SYNAPSE;

ANIMALS; CELL MEMBRANE; CELLS, CULTURED; DENDRITES; DIFFUSION; FLUORESCENCE; FLUORESCENT DYES; MICROSCOPY, ELECTRON; NANOTECHNOLOGY; NEURITES; NEURONS; PYRIDINIUM COMPOUNDS; QUATERNARY AMMONIUM COMPOUNDS; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, GLYCINE; SEMICONDUCTORS; SPINAL CORD; SYNAPSES;

EID: 0142116238     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1088525     Document Type: Article

References (30)

1. Special issue on Biological Imaging, S. M. Hurtley, L. Helmuth, Eds., Science 300 (4 April 2003).
2. M. Saxton, K. Jacobson, Annu. Rev. Biophys. Biomol. Struct. 26, 373 (1997).
3. M. Ueda, Y. Sako, T. Tanaka, P. Devreotes, T. Yanagida, Science 294, 864 (2001).
4. G. S. Harms, L. Cognet, P. H. M. Lommerse, G. A. Blab, T. Schmidt, Biophys. J. 80, 2396 (2001).
5. S. Empedocles, M. G. Bawendi, Acc. Chem. Res. 32, 389 (1999).
6. W. Chan et al., Curr. Opin. Biotechnol. 13, 40 (2002).
7. X. Wu et al., Nature Biotechnol. 21, 41 (2003).
8. B. Dubertret et al., Science 298, 1759 (2002).
9. D. R. Larson et al., Science 300, 1434 (2003).
10. K. Harvey, J. von der Nuell, R. J. Harvey, in Receptor and Ion-Channel Trafficking, S. J. Moss, J. Henley, Eds. (Oxford Univ. Press, New York, 2002), chap. 8.
11. J. Kirsch, I. Wolters, A. Triller, H. Betz, Nature 366, 745 (1993).
12. G. Feng et al., Science 282, 1321 (1998).
13. D. Choquet, A. Triller, Nature Rev. Neurosci. 4, 251 (2003).
14. J. Meier, C. Vannier, A. Sergé, A. Triller, D. Choquet, Nature Neurosci. 4, 253 (2001).
15. A. J. Borgdorff, D. Choquet, Nature 417, 649 (2002).
16. A. Sergé, L. Fourgeaux, A. Hemar, D. Choquet, J. Neurosci. 22, 3910 (2002).
17. Materials and methods are available as supporting material on Science Online.
18. A. Dumoulin, S. Levi, B. Riveau, B. Gasnier, A. Triller, Eur. J. Neurosci. 12, 3883 (2000).
19. M. Nirmal et al., Nature 383, 802 (1996).
20. R. E. Thomson, D. R. Larson, W. W. Webb, Biophys. J. 82, 2775 (2002).
21. A. Triller, F. Cluzeaud, F. Pfeiffer, H. Betz, H. Korn, J. Cell. Biol. 101, 683 (1985).
22. SQDT measurements were performed at room temperature. Additional experirnents at 37°C led to similar findings (17).
23. The Kolmogorov-Smirnov test indicated significant differences between the distributions of D in the different domains (P < 0.0001).
24. At glutamatergic synapses, rapid and slow diffusion of AMPA receptors have been recently described with comparable diffusion coefficient values [C. Tardin, L. Cognet, C. Bats, B. Lounis, D. Choquet, EMBO. J. 22, 4656 (2003)].
25. 2/s (n = 90). The fraction of rapid receptors was 27 and 12% for perisynaptic and synaptic GlyRs, respectively (Fig. 3F).
26. S. Schroder, W. Hoch, C. M. Becker, G. Grenningloh, H. Betz, Biochemistry 30, 42 (1991).
27. J. E. Gready, S. Ranganathan, P. R. Schofield, Y. Matsuo, K. Nichikawa, Protein Sci. 6, 983 (1997).
28. N. Unwin, Cell 72, 31 (1993).
29. H. Rasmussen, T. Rasmussen, A. Triller, C. Vannier, Mol. Cell. Neurosci. 19, 201 (2002).
30. We thank C. Hanus, M. Humbert, M. V. Ehrensperger, C. Charrier, and C. Vannier for their help and for fruitful discussions. Supported by CNRS, INSERM, and Ministère de la Recherche (Action Concertée Dynamique et Réactivité des Assemblages Biologiques and Action Concertée Nanostructures). C.L. was supported by a Marie Curie Host Fellowship Contract (HPMT-CT-2000-00102).