XCTK2: A kinesin-related protein that promotes mitotic spindle assembly in Xenopus laevis egg extracts

Journal of Cell Biology

Volumn 136, Issue 4, 1997, Pages 859-870

  Walczak, Claire E ^a,b   Verma, Suzie ^a   Mitchison, Timothy J ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; GLOBULAR PROTEIN; KINESIN;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; EGG; MITOSIS SPINDLE; NONHUMAN; PRIORITY JOURNAL; XENOPUS LAEVIS;

AMINO ACID SEQUENCE; ANIMALS; CELL EXTRACTS; KINESIN; MACROMOLECULAR SUBSTANCES; MITOTIC SPINDLE APPARATUS; MOLECULAR SEQUENCE DATA; OVUM; XENOPUS LAEVIS; XENOPUS PROTEINS;

ANIMALIA; INSECTA; UNIDENTIFIED BACULOVIRUS; XENOPUS LAEVIS;

EID: 0031020735     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.136.4.859     Document Type: Article

References (54)

1. Ando, A., Y.Y. Kikuti, H. Kawata, N. Okamoto, T. Imai, T. Eki, K. Yokoyama, E. Soeda, T. Ikemura, K. Abe, et al. 1994. Cloning of a new kinesin-related gene located at the centromeric end of the human MHC region. Immunogenetics. 39:194-200.
2. Barton, N.R., and L.S.B. Goldstein. 1996. Going mobile: microtubule motors and chromosome segregation. Proc. Natl. Acad. Sci. USA. 93:1735-1742.
3. Barton, N.R., A.J. Pereira, and L.S.B. Goldstein. 1995. Motor activity and mitotic spindle localization of the Drosophila kinesin-like protein KLP61F. Mol. Biol. Cell. 6:1563-1574.
4. Bloom, G., and S. Endow. 1995. Motor proteins 1: kinesins. Protein Profile. 2: 1109-1171.
5. Boleti, H., E. Karsenti, and I. Vernos. 1996. Xklp2, a novel Xenopus centrosomal kinesin-like protein required for centrosome separation during mitosis. Cell. 84:49-59.
6. Chandra, R., E.D. Salmon, H.P. Erickson, A. Lockhart, and S.A. Endow. 1993. Structural and functional domains of the Drosophila ncd microtubule motor protein. J. Biol. Chem. 268:9005-9013.
7. Cole, D.G., and J.M. Scholey. 1995. Structural variations among the kinesins. Trends Cell Biol. 5:259-262.
8. Cole, D.G., W.M. Saxton, K.B. Sheehan, and J.M. Scholey. 1994. A slow homotetrameric kinesin-related motor protein purified from Drosophila embryos. J. Biol. Chem. 269:22913-22916.
9. Davis, D.G. 1969. Chromosome behavior under the influence of claret-nondisjunctional in Drosophila melanogaster. Genetics. 61:577-594.
10. Echeverri, C.J., B.M. Paschal, K.T. Vaughan, and R.B. Vallee. 1996. Molecular characterization of the 50-kD subunit of dynactin reveals function for the complex in chromosome alignment and spindle organization during mitosis. J. Cell Biol. 132:617-633.
11. Endow, S.A., S.J. Kang, L.L. Satterwhite, M.D. Rose, V.P. Skeen, and E.D. Salmon. 1994. Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends. EMBO (Eur. Mol. Biol. Organ.) J. 13:2708-2713.
12. Enos, A.P., and N.R. Morris. 1990. Mutation of a gene that encodes a kinesin-like protein blocks nuclear division in A. nidulans. Cell. 60:1019-1027.
13. Eshel, D., L.A. Urrestarazu, S. Vissers, J. Jauniaux, J.C. van Vliet-Reedijk, R.J. Planta, and I.R. Gibbon. 1993. Cytoplasmic dynein is required for normal nuclear segregation in yeast. Proc. Natl. Acad. Sci. USA. 90:11172-11176.
14. Gaglio, T., A. Saredi, and D.A. Compton. 1995. NuMA is required for the organization of microtubules into aster-like mitotic arrays. J. Cell Biol. 131:693-708.
15. Gaglio, T., A. Saredi, J. Bingham, J. Hasbani, S.R. Gill, T.A. Schroer, and D.A. Compton. 1996. Opposing motor activities are required for the organization of the mammalian mitotic spindle pole. J. Cell Biol. 135:399-414.
16. Hagan, I., and M. Yanagida. 1992. Kinesin-related cut7 protein associates with mitotic and meiotic spindles in fission yeast. Nature (Lond.). 356:74-76.
17. Harlow, E., and D. Lane. 1988. Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
18. Hatsumi, M., and S.A. Endow. 1992a. The Drosophila ncd microtubule motor protein is spindle-associated in meiotic and mitolic cells. J. Cell Sci. 103:1013-1020.
19. Hatsumi, M., and S.A. Endow. 1992b. Mutants of the microtubule motor protein, nonclaret disjunctional, affect spindle structure and chromosome movement in meiosis and mitosis. J. Cell Sci. 101:547-559.
20. Heald, R., R. Tournebize, T. Blank, R. Sandaltzopoulos, P. Becker, A. Hyman, and W. Karsenti. 1996. Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts. Nature (Lond.). 382:420-425.
21. Hirano, T., and T.J. Mitchison. 1993. Topoisomerase II does not play a scaffolding role in the organization of mitotic chromosomes assembled in Xenopus egg extracts. J. Cell Biol. 120:601-612.
22. Hoyt, M.A., L. He, K.K. Loo, and W.S. Saunders. 1992. Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly. J. Cell Biol. 118:109-120.
23. Hoyt, M.A., L. He, L. Totis, and W.S. Saunders. 1993. Loss of function of Saccharomyces cerevisiae kinesin-related CIN8 and KIP1 is suppressed by KAR3 motor domain mutations. Genetics. 135:35-44.
24. Kashina, A.S., J.M. Scholey, J.D. Leszyk, and W.M. Saxton. 1996. An essential bipolar mitotic motor. Nature (Lond.). 384:225.
25. Kuriyama, R., M. Kofron, R. Essner, T. Kato, S. Dragas-Granoic, C.K. Omoto, and A. Khodjakov. 1995. Characterization of a minus end-directed kinesin-like motor protein from cultured mammalian cells. J. Cell Biol. 129:1049-1059.
26. Li, Y., E. Yeh, T. Hays, and K. Bloom. 1993. Disruption of mitotic spindle orientation in a yeast dynein mutant. Proc. Natl. Acad. Sci. USA. 90:10096-10100.
27. Lohka, M.J., and J.L. Maller. 1985. Induction of nuclear envelope breakdown, chromosome condensation, and spindle formation in cell-free extracts. J. Cell Biol. 101:518-523.
28. Matthies, H.J.G., H.B. McDonald, L.S.B. Goldstein, and W.E. Theurkauf. 1996. Anastral meiotic spindle morphogenesis: role of the nonclaret disjunctional kinesin-like protein. J. Cell Biol. 134:455-464.
29. McDonald, H.B., R.J. Stewart, and L.S.B. Goldstein. 1990. The kinesin like ncd protein is a minus end directed microtubule motor. Cell. 63:1159-1165.
30. Meluh, P.B., and M.D. Rose. 1990. KAR3, a kinesin-related gene required for yeast nuclear fusion. Cell. 60:1029-1041.
31. Merdes, A., K. Ramyar, J.D. Vechio, and D.W. Cleveland. 1996. A complex of NuMA and cytoplasmic dynein is essential for mitotic spindle assembly. Cell. 87:447-458.
32. Moore, J.D., and S.A. Endow. 1995. Kinesin proteins: a phylum of motors for microtubule-based motility. Bioessays. 18:207-219.
33. Murray, A.W. 1991. Cell cycle extracts. In Methods in Cell Biology. Vol. 36. B.K. Kay and H.B. Peng, editors. Academic Press Inc., San Diego, CA. pp. 581-605.
34. O'Connell, M.J., P.B. Meluh, M.D. Rose, and N.R. Morris. 1993. Suppression of the bimC4 mitotic spindle defect by deletion of klpA, a gene encoding a kar3-related kinesin-like protein in Aspergillus nidulans. J. Cell Biol. 120:153-162.
35. Page, B.D., L.L. Satterwhite, M.D. Rose, and M. Snyder. 1994. Localization of the Kar3 kinesin heavy chain-related protein requires the Cik1 interacting protein. J. Cell Biol. 124:507-519.
36. Pfarr, C.M., M. Coue, P.M. Grisson, T.S. Hays, M.E. Porter, and J.R. McIntosh. 1990. Cytoplasmic dynein is localized to kinetochores during mitosis. Nature (Lond.). 345:263-265.
37. Pidoux, A.L., M. LeDizet, and W.Z. Cande. 1996. Fission yeast pkl1 is a kinesin-related protein involved in mitotic spindle function. Mol. Biol. Cell. 7:1639-1655.
38. Roof, D.M., P.B. Meluh, and M.D. Rose. 1992. Kinesin-related proteins required for assembly of the mitotic spindle. J. Cell Biol. 118:95-108.
39. Saunders, W.S., and M.A. Hoyt. 1992. Kinesin-related proteins required for structural integrity of the mitotic spindle. Cell. 70:451-458.
40. Saunders, W.S., D. Koshland, D. Eshel, I.R. Gibbons, and M.A. Hoyt. 1995. Saccharomyces cerevisiae kinesin- and dynein-related proteins required for anaphase chromosome segration. J. Cell Biol. 128:617-624.
41. Sawin, K.E., and T.J. Mitchison. 1991. Mitotic spindle assembly by two different pathways in vitro. J. Cell Biol. 112:925-940.
42. Sawin, K.E., and S.A. Endow. 1993. Meiosis, mitosis and microtubule motors. Bioessays. 15:399-407.
43. Sawin, K.E., K. LeGuellec, M. Philippe, and T.J. Mitchison. 1992a. Mitotic spindle organization by a plus-end directed microtubule motor. Nature (Lond.). 359:540-543.
44. Sawin, K.E., T.J. Mitchison, and L.G. Wordeman. 1992b. Evidence for kinesin-like proteins in the mitotic apparatus using peptide antibodies. J. Cell Sci. 102:303-313.
45. Shamu, C.E., and A.W. Murray. 1992. Sister chromatid separation in frog egg extracts requires DNA topoisomerase II activity during anaphase. J. Cell Biol. 117:921-934.
46. Siegel, L.W., and K.J. Monty. 1966. Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases. Biochim. Biophys. Acta. 112:346-362.
47. Smith, D.B., M.R. Rubira, R.J. Simpson, K.M. Davern, W.U. Tui, P.G. Board, and G.F. Mitchell. 1988. Expression of an enzymatically-active parasite molecule in Escherichia coli: Schistosoma japonicum glutathione S-transferase. Mol. Biochem. Parasitol. 27:249-256.
48. Steuer, E.R., L. Wordeman, T.A. Schroer, and M.P. Sheetz. 1990. Localization of cytoplasmic dynein to mitotic spindles and kinetochores. Nature (Lond.). 345:266-268.
49. Vaisberg, E.A., M.P. Koonce, and J.R. McIntosh. 1993. Cytoplasmic dynein plays a role in mammalian mitotic spindle formation. J. Cell Biol. 123:849-858.
50. Verde, F., J.-M. Berrez, C. Antony, and E. Karsenti. 1991. Taxol-induced microtubule asters in mitotic extracts of Xenopus eggs: requirement for phosphorylated factors and cytoplasmic dynein. J. Cell Biol. 112:1177-1188.
51. Vernos, I., and E. Karsenti. 1996. Motors involved in spindle assembly and chromosome segregation. Curr. Opin. Cell Biol. 8:4-9.
52. Vernos, I., J. Raats, T. Hirano, J. Heasman, E. Karsenti, and C. Wylie. 1995. Xklp1, a chromosomal Xenopus kinesin-like protein essential for spindle organization and chromosome positioning. Cell. 81:117-127.
53. Walczak, C.E., T.J. Mitchison, and A. Desai. 1996. XKCM1: A Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly. Cell. 84:37-47.
54. Walker, R.A., E.D. Salmon, and S.A. Endow. 1990. The Drosophila claret segregation protein is a minus-end directed motor molecule. Nature (Lond.). 347:780-782.