Genotoxic damage in hospital workers exposed to ionizing radiation and metabolic gene polymorphisms

Journal of Toxicology and Environmental Health - Part A: Current Issues

Volumn 75, Issue 13-15, 2012, Pages 934-946

  Sakly, Amina ^a   Gaspar, Jorge Francisco ^b   Kerkeni, Emna ^a   Silva, Suzana ^c   Teixeira, João Paulo ^c   Chaari, Neila ^d   Cheikh, Hassen Ben ^a  

^a UNIVERSITY OF MONASTIR   (Tunisia)

^b UNIVERSIDADE NOVA DE LISBOA   (Portugal)

^c NATIONAL INSTITUTE OF HEALTH   (Portugal)

^d EPS Fattouma Bourguiba of Monastir ^*  (Tunisia)

Author keywords

[No Author keywords available]

Indexed keywords

GLUTATHIONE TRANSFERASE M1; GLUTATHIONE TRANSFERASE P1; GLUTATHIONE TRANSFERASE T1;

ADULT; CHROMOSOME DAMAGE; CHROMOSOME SIZE; COMET ASSAY; CONFERENCE PAPER; CONTROLLED STUDY; CORRELATION ANALYSIS; FEMALE; GENETIC POLYMORPHISM; GENOTOXICITY; HOSPITAL PERSONNEL; HUMAN; HUMAN CELL; IONIZING RADIATION; MAJOR CLINICAL STUDY; MALE; MICRONUCLEUS; OCCUPATIONAL DISEASE; PERIPHERAL LYMPHOCYTE; PRIORITY JOURNAL; RADIATION INJURY; XENOBIOTIC METABOLISM;

ACCIDENTS, OCCUPATIONAL; ADULT; CHROMOSOMES, HUMAN; DNA DAMAGE; FEMALE; GENETIC ASSOCIATION STUDIES; GLUTATHIONE S-TRANSFERASE PI; GLUTATHIONE TRANSFERASE; HOSPITALS, URBAN; HUMANS; INCIDENCE; LYMPHOCYTES; MALE; MICRONUCLEI, CHROMOSOME-DEFECTIVE; MIDDLE AGED; PERSONNEL, HOSPITAL; POLYMORPHISM, GENETIC; RADIATION INJURIES; RADIOACTIVE HAZARD RELEASE; RADIOLOGY DEPARTMENT, HOSPITAL; TUNISIA;

EID: 84864037516     PISSN: 15287394     EISSN: 10872620     Source Type: Journal    
DOI: 10.1080/15287394.2012.690710     Document Type: Conference Paper

References (82)

1. Aka, P., Mateuca, R., Buchet, J. P., Thierens, H., and, Kirsch-Volders, M. 2004. Are genetic polymorphisms in OGG1, XRCC1 and XRCC3 genes predictive for the DNA strand break repair phenotype and genotoxicity in workers exposed to low dose ionising radiations?. Mutat. Res., 556: 169-81.
2. Andreoli, C., Rossi, S., Leopardi, P., and, Crebelli, R. 1999. DNA damage by hydroquinone in human white blood cells: Analysis by alkaline single-cell gel electrophoresis. Mutat. Res., 438: 37-45.
3. Angelini, S., Kumar, R., Carbone, F., Maffei, F., Forti, G. C., Violante, F. S., Lodi, V., Curti, S., Hemminki, K., and, Hrelia, P. 2005. Micronuclei in humans induced by exposure to low level of ionizing radiation: Influence of polymorphisms in DNA repair genes. Mutat. Res., 570: 105-17.
4. Barquinero, J. F., Barrios, L., Caballin, M. R., Miro, R., Ribas, M., Subias, A., and, Egozcue, J. 1993. Cytogenetic analysis of lymphocytes from hospital workers occupationally exposed to low levels of ionizing radiation. Mutat. Res., 286: 275-79.
5. Berrington, A., Darby, S. C., Weiss, H. A., and, Doll, R. 2001. 100 years of observation on British radiologists: Mortality from cancer and other causes 1897-1997. Br. J. Radiol., 74: 507-19.
6. Betti, C., Davini, T., Giannessi, L., Loprieno, N., and, Barale, R. 1995. Comparative studies by comet test and SCE analysis in human lymphocytes from 200 healthy subjects. Mutat. Res., 343: 201-7.
7. Betti, C., Davini, T., Giannessi, L., Loprieno, N., and, Barale, R. 1994. Microgel electrophoresis assay (comet test) and SCE analysis in human lymphocytes from 100 normal subjects. Mutat. Res., 307: 323-33.
8. Bonassi, S., Forni, A., Bigatti, P., Canevarollo, N., De Ferrari, M., Lando, C., Padovani, P., Bevegni, M., Stella, M., Vecchio, D., and, Puntoni, R. 1997. Chromosome aberrations in hospital workers: Evidence from surveillance studies in Italy (1963-1993). Am. J. Ind. Med., 31: 353-60.
9. Bukvic, N., Gentile, M., Susca, F., Fanelli, M., Serio, G., Buonadonna, L., Capurso, A., and, Guanti, G. 2001. Sex chromosome loss, micronuclei, sister chromatid exchange and aging: A study including 16 centenarians. Mutat. Res., 498: 159-67.
10. Cardis, E., Vrijheid, M., Blettner, M., Gilbert, E., Hakama, M., Hill, C., Howe, G., Kaldor, D., Muirhead, C.R., Schubauer-Berigan, M., Yoshimura, T., Bermann, F., Cowper, G., Fix, J., Hacker, C., Heinmiller, B., Marshall, M., Thierry-Chef, I., Utterback, D., Ahn, Y. O., Amoros, E., Ashmore, P., Auvinen, A., Bae, J.-M., Bernar Solano, J., Biau, A., Combalot, E., Deboodt, P., Diez Sacristan, A., Eklof, M., Engels, H., Engholm, G., Gulis, G., Habib, R., Holan, K., Hyvonen, H., Kerekes, A., Kurtinaitis, J., Malker, H., Martuzzi, M., Mastauskas, A., Monnet, A., Moser, M., Pearce, M.S., Richardson, D. B., Rodriguez-Artalejo, F., Rogel, A., Tardy, H., Telle-Lamberton, M., Turai, I., Usel, M., and, Veress, K. 2005. Risk of cancer after low doses of ionising radiation: retrospective cohort study in 15 countries. Br. Med. J., 331: 77-80.
11. Cardoso, R. S., Takahashi-Hyodo, S., Peitl, P. Jr., Ghilardi-Neto, T., and, Sakamoto-Hojo, E. T. 2001. Evaluation of chromosomal aberrations, micronuclei, and sister chromatid exchanges in hospital workers chronically exposed to ionizing radiation. Teratogen. Carcinogen. Mutagen., 21: 431-39.
12. Carere, A., Antoccia, A., Cimini, D., Crebelli, R., Degrassi, F., Leopardi, P., Marcon, F., Sgura, A., Tanzarella, C., and, Zijno, A. 1999. Analysis of chromosome loss and non-disjunction in cytokinesis-blocked lymphocytes of 24 male subjects. Mutagenesis, 14: 491-96.
13. Chung, H. W., Ryu, E. K, Kim, Y. J., and, Ha, S. W. 1996. Chromosome aberrations in workers of nuclear-power plants. Mutat. Res., 350: 307-14.
14. Da Cruz, A. D., McArthur, A. G., Silva, C. C., Curado, M. P., and, Glickman, B. W. 1994. Human micronucleus counts are correlated with age, smoking and cesium-137 dose in the Goiana (Brazil) radiological accident. Mutat. Res., 313: 57-68.
15. Dias, F. L., Antunes, L. M. G., Rezende, P. A., Carvalho, F. E. S., Silva, C. M. D., Matheus, J. M., Oliveira, J. V. Jr, Lopes, G. P., Pereira, G. A., and, Balarin, M. A. S. 2007. Cytogenetic analysis in lymphocytes from workers occupationally exposed to low levels of ionizing radiation. Environ. Toxicol. Pharmacol., 23: 228-33.
16. Dusinska, M., Ficek, A., Horska, A., Raslova, K., Petrovska, H., Vallova, B., Drlickova, M., Wood, S. G., Stupakova, A., Gasparovic, J., Bobek, P., Nagyova, A., Kovacikova, Z., Blazicek, P., Liegebel, U., and, Collins, A. R. 2001. Glutathione S -transferase polymorphisms influence the level of oxidative DNA damage and antioxidant protection in humans. Mutat. Res., 482: 47-55.
17. Fenech, M. 1998. Important variables that influence base-line micronucleus frequency in cytokinesis-blocked lymphocytes-a biomarker for DNA damage in human populations. Mutat. Res., 404: 155-65.
18. Fenech, M., and, Morley, A. 1985. Measurement of micronuclei in lymphocytes. Mutat. Res., 147: 29-36.
19. Fenech, M., and, Morley, A. 1986. Cytokinesis-block micronuclei method in human lymphocytes: Effect of in vivo aging and low dose X-irradiation. Mutat. Res., 161: 193-98.
20. Fenech, M., Neville, S., and, Rinaldi, J. 1994. Sex is an important variable affecting spontaneous micronucleus frequency in cytokinesis blocked lymphocytes. Mutat. Res., 313: 203-7.
21. Frenzilli, G., Betti, C., Davini, T., Desideri, M., Fornai, E., Giannessi, L., Maggiorelli, F., Paoletti, P., and, Barale, R. 1997. Evaluation of DNA damage in leukocytes of ex-smokers by single cell gel electrophoresis. Mutat. Res., 375: 117-23.
22. Frenzilli, G., Lori, A., Panasiuk, G., Ferdeghini, M., and, Barale, R. 1998. Comet assay on children's leukocytes 8 years after the Chernobyl disaster. Mutat. Res., 415: 152-58.
23. Fucic, A., Markucic, D., Mijic, A., and, Jazbec, A. M. 2000. Estimation of genome damage after exposure to ionizing radiation and ultrasound used in industry. Environ. Mol. Mutagen., 36: 47-51.
24. Garaj-Vrhovac, V., Fucic, A., Kubelka, D., and, Hebrang, A. 1997. Assessment of genome damage in occupational exposure to ionizing radiation and ultrasound. Mutat. Res., 395: 101-5.
25. Ginsberg, G., Smolenski, S., Hattis, D., Guyton, K. Z., Johns, D. O., and, Sonawane, B. 2009. Genetic polymorphism glutathione transferases (GST): Population distribution if GSTM1 T1, and P1 conjugating activity. J. Toxicol. Environ. Health B, 12: 389-439.
26. Hadjidekova, V. B., Bulanova, M., Bonassi, S., and, Neri, M. 2003. Micronucleus frequency is increased in peripheral blood lymphocytes of nuclear power plant workers. Radiat. Res., 160: 684-90.
27. Hagelstrm, A. H., Gorla, N. B., and, Larripa, I. B. 1995. Chromosomal damage in workers occupationally exposed to chronic low level ionizing radiation. Toxicol. Lett., 76: 113-17.
28. Hando, J. C., Tucker, J. D., Davenport, M., Tepperberg, J., and, Nath, J. 1997. X chromosome inactivation and micronuclei in normal and Turner individuals. Hum. Genet., 100: 624-28.
29. Harries, M., Coggan, M., Langton, L., Wilson, S., and, Board, P. 1998. Polymorphisms of the pi class glutathione S-transferase in normal populations and cancer patients. Pharmacogenetics, 8: 27-31.
30. Heimers, A. 2000. Chromosome aberration analysis in Concorde pilots. Mutat. Res., 467: 169-76.
31. Higami, Y., Shimokawa, I., Okimoto, T., and, Ikeda, T. 1994. An age-related increase in the basal level of DNA damage and DNA vulnerability to oxygen radicals in the individual hepatocytes of male F344 rats. Mutat. Res., 316: 59-67.
32. Joksic, G., and, Spasojevic-Tisma, V. 1988. Chromosome analysis of lymphocytes from radiation workers in tritium-applying. Int. Arch. Occup. Environ. Health, 71: 213-20.
33. Joseph, L. J., Patwardhan, U. M., and, Samuel, A. B. 2004. Frequency of micronuclei in peripheral blood lymphocytes from subjects occupationally exposed to low levels of ionizing radiation. Mutat. Res., 564: 83-88.
34. Karahalil, B., Sardas, S., Kocabas, N. A., Alhayiroglu, E., Karakaya, A. E., and, Civelek, E. 2002. Chromosomal aberrations under basal conditions and after treatment with x-ray in human lymphocytes as related to the GSTM1 genotype. Mutat. Res., 515: 135-40.
35. Ketterer, B. 1998. Glutathione S-tranferases and prevention of cellular free radical damage. Free. Radical Res., 28: 647-58.
36. Kopjar, N., and, Garaj-Vrhovac, V. 2005. Assessment of DNA damage in nuclear medicine personnelComparative study with the alkaline comet assay and the chromosome aberration test. Int. J. Hyg. Environ. Health, 208: 179-91.
37. Krewski, D., Lubin, J. H., Zielinski, J. M., Alavanja, M., Catalan, V. S., Field, R. W., Klotz, J. B., Letourneau, E. G., Lynch, C. F., Lyon, J. L., Sandler, D. P, Schoenberg, J. B., Steck, D. J., Stolwijk, J. A., Weinberg, C., and, Wilcox, H. B. 2006. A combined analysis of North American case-control studies of residential radon and lung cancer. J. Toxicol. Environ. Health A, 69: 533-97.
38. Lin, D., Tang, Y., Peng, Q., Lu, S., Ambrosone, C. B., and, Kadublar, F. F. 1998. Susceptibility to esophageal cancer and genetic polymorphisms in glutatione- S-transferase T1, P1 and M1 and cytochrome P450 2E1. Cancer Epidemiol. Biomarkers Prev., 7: 1013-18.
39. Maffei, F., Angelini, S., Cantelli Forti, G., Lodi, V., Violante, F. S., Mattioli, S., and, Hrelia, P. 2002. Micronuclei frequencies in hospital workers occupationally exposed to low levels of ionizing radiation: influence of smoking status and other factors. Mutagenesis, 17: 405-9.
40. Maitre, A., Colonna, M., Gressin, C., Menegoz, F., and, Gaudemaris, R. 2003. Increased incidence of haematological cancer among physicians in a University Hospital. Int. Arch. Occup. Environ. Health, 76: 24-28.
41. Maluf, S. W., Passos, D. F., Bacelar, A., Speit, G., and, Erdtmann, B. 2001. Assessment of DNA damage in lymphocytes of workers exposed to -radiation using the micronucleus test and the comet assay. Environ. Mol. Mutagen., 38: 311-15.
42. Mateuca, R., Lombaert, N., Aka, P. V., Decordier, I., and, Kirsch-Volders, M. 2006. Chromosomal changes: Induction, detection methods and applicability in human biomonitoring. Biochemistry, 88: 1515-31.
43. Mayer, C., Popanda, O., Zelezny, O., von Brevern, M. C., Bach, A., Bartsch, H., and, Schmetzer, P. 2002. DNA repair capacity after g-irradiation and expression profiles of DNA repair genes in resting and proliferating human peripheral blood lymphocytes. DNA Repair, 1: 237-50.
44. Migliore, L., Parrini, M., Sbrana, I., Biagini, C., Battaglia, A., and, Loprieno, N. 1991. Micronucleated lymphocytes in people occupationally exposed to potential environmental contaminants: the age effect. Mutat. Res., 256: 13-19.
45. Miller, S. A., Dykes, D. D., and, Polesky, H. F. 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res., 16: 12-15.
46. Moller, P., Knudsen, L. E., Loft, S., and, Wallin, H. 2000. The comet assay as rapid test in biomonitoring occupational exposure to DNA-damaging agents and effect of confounding factors. Cancer Epidemiol. Biomarkers Prev., 9: 1005-15.
47. Nadin, S. B., Vargas-Roig, L. M., and, Ciocca, D. R. 2001. A silver stain method for single-cell gel assay. J. Histochem. Cytochem., 49: 1183-86.
48. Norman, A., Bass, D., and, Roe, D. 1985. Screening human populations for chromosome aberrations. Mutat. Res., 143: 155-60.
49. Norppa, H., and, Falck, G. C. 2003. What do the micronuclei contain?. Mutagenesis, 18: 221-33.
50. Olive, P. L. 1999. DNA damage and repair in individual cells: applications of the comet assay in radiobiology. Int. J. Radiat. Biol., 75: 395-405.
51. stling, O., and, Johanson, K. J. 1984. Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem. Biophys. Res. Commun., 123: 291-98.
52. Palus, J., Dziubaltowska, E., and, Rydzynski, K. 1999. DNA damage detected by the comet assay in the white blood cells of workers in a wooden furniture plant. Mutat. Res., 444: 61-74.
53. Park, S., Yoo, K., Lee, S., Kim, S., Ahn, S., Noh, K., Strickland, P., Hirvonen, A., and, Kang, D. 2000. Alcohol consumption, glutathione S -transferase M1 and T1 genetic polymorphisms and breast cancer risk. Pharmacogenetics, 10: 301-9.
54. Piperakis, S. M., Visvardis, E. E., Sagnou, M., and, Tassiou, A. M. 1998. Effects of smoking and aging on oxidative DNA damage of human lymphocytes. Carcinogenesis, 19: 695-98.
55. Ramos, D. L., Gaspar, J. F., Pingarilho, M., Gil, O. M., Fernandes, A. S., Rueff, J., and, Oliveira, N. G. 2011. Genotoxic effects of doxorubicin in cultured human lymphocytes with different glutathione S -transferase genotypes. Mutat. Res., 724: 28-34.
56. Richard, F., Muleris, M., and, Dutrillaux, B. 1994. The frequency of micronuclei with X chromosome increases with age in human females. Mutat. Res., 316: 1-7.
57. Rojas, E., Lopez, M. C., and, Valverde, M. 1999. Single cell gel electrophoresis assay: Methodology and applications. J. Chromatogr. B. Biomed. Sci. Appl., 722: 225-54.
58. Rydberg, D., Skaung, V., Hewer, A., Philiphs, D. H., Harries, L. W., Wolf, C. R., Ogreid, D., Ulvik, A., Vu, P., and, Haugen, A. 1997. Genotypes of glutathione transferase M1 and P1 and their significance for adduct levels and cancer risk. Carcinogenesis, 18: 1285-89.
59. Sankaranarayanan, K. 1988. Prevalence of genetic and partially genetic disease in man and the estimation of genetic risks of exposure to ionizing radiation. Am. J. Hum. Genet, 42: 651-62.
60. Sari-Minodier, I., Orsiere, T., Bellon, L., Pompili, J., Sapin, C., and, Botta, A. 2002. Cytogenetic monitoring of industrial radiographers using the micronucleus assay. Mutat. Res., 521: 37-46.
61. Silva, S. N., Gil, O. M, Oliveira, V. C., Cabral, M. N., Azevedo, A. P., Faber, A., Manita, I., Ferreira, T. C., Limbert, E., Pina, J. E., Rueff, J., and, Gaspar, J. 2005. Association of polymorphisms in ERCC2 gene with non-familial thyroid cancer risk. Cancer Epidemiol Biomarkers Prev., 14: 2407-12.
62. Singh, N. P., Danner, D. B., Tice, R. R., Pearson, J. D., Brant, L. J., Morrell, C. H., and, Schneider, E. L. 1991. Basal DNA damage in individual human lymphocytes with age. Mutat. Res., 256: 1-6.
63. Singh, N. P. 2000. Microgels for estimation of DNA strand breaks, DNA protein crosslinks and apoptosis. Mutat. Res., 455: 111-27.
64. Singh, N. P., Danner, D. B., Tice, R. R., Brant, L., and, Schneider, E. L. 1990. DNA damage and repair with age in individual human lymphocytes. Mutat. Res., 237: 123-30.
65. Singh, N. P., McCoy, M. T., Tice, R. R., and, Schneider, E. L. 1988. A simple technique for quantification follows levels of DNA damage in individual cells. Exp. Cell Res., 175: 184-91.
66. Sram, R. J., Rossner, P., Rubes, J., Beskid, O., Dusek, Z., Chvatalova, I., Schmuczerova, J., Milcova, A., Solansky, I., Bavorova, H., Ocadlikova, D., Kopecna, O., and, Musilova, P. 2006. Possible genetic damage in the Czech nuclear power plant workers. Mutat. Res., 593: 50-63.
67. Strange, R., Jones, P., and, Freyer, A. 2000. Glutathione S -transferase: Genetics and role in toxicology. Toxicol. Lett., 112-13: 357-63.
68. Teixeira, J. P., Gaspar, J., Martinho, G., Silva, S., Rodrigues, S., Mayan, O., Martin, E., Farmer, P. B., and, Rueff, J. 2002. Aromatic DNA adduct levels in coke oven workers: Correlation with polymorphisms in genes GSTP1, GSTM1, GSTT1 and CYP1A1. Mutat. Res., 517: 147-55.
69. Thierens, H., Vral, A., and, De Ridder, L. 1996. A cytogenetic study of radiological workers: effect of age, smoking and radiation burden on the micronucleus frequency. Mutat. Res., 360: 75-82.
70. Thierens, H., Vral, A., Morthier, R., Aousalah, B., and, De Ridder, L. 2000. Cytogenetic monitoring of hospital workers occupationally exposed to ionizing radiation using the micronucleus centromere assay. Mutagenesis, 15: 245-49.
71. Tice, R. R., Agurell, E., Anderson, D., Burlinson, B., Hartmann, A., Kobayashi, H., Miyamae, Y., Rojas, E., Ryu, J. C., and, Sasaki, Y. F. 2000. Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen., 35: 206-21.
72. Touil, N., Aka, P. V., Buchet, J. P., Thierens, H., and, Kirsch-Volders, M. 2002. Assessment of genotoxic effects related to chronic low level exposure to ionizing radiation using biomarkers for DNA damage and repair. Mutagenesis, 17: 223-32.
73. Tracy, B. L., Krewski, D., Chen, J., Zielinski, J. M., Brand, K. P., and, Meyerhof, D. 2006. Assessment and management of residential radon health risks: A report from the Health Canada radon workshop. J. Toxicol. Environ. Health A, 69: 735-58.
74. UNSCEAR. 2000. Sources and effects of ionizing radiation. Report, vol. 1. United Nations Scientific Committee on the Effects of Atomic Radiation http://www.unscear.org
75. Vijayalaxmi, R. R., Tice, G. H., and, Struss, S. 1992. Assessment of radiation-induced DNA damage in human blood lymphocytes using the single-cell gel electrophoresis technique. Mutat. Res., 271: 243-52.
76. Wang, J. X., Zhang, L. A., Li, B. X., Zhao, Y. C., Wang, Z. Q., Zhang, J. Y., and, Aoyama, T. 2002. Cancer incidence and risk estimation among medical X-ray workers in China, 1950-1995. Health Phys., 82: 455-66.
77. Wojewodzka, M., Kruszewski, M., Iwanenko, T., Collins, A. R., and, Szumiel, I. 1998. Application of the comet assay for monitoring DNA damage in workers exposed to chronic low-dose irradiation. I. Strand breakage. Mutat. Res., 416: 21-35.
78. Wojewodzka, M., Kruszewski, M., Iwanenko, T., Collins, A. R., and, Szumiel, I. 1999. Lack of adverse effect of smoking habit on DNA strand breakage and base damage, as revealed by the alkaline comet assay. Mutat. Res., 440: 19-25.
79. Yoshinaga, S., Aoyama, T., Yoshimoto, Y., and, Sugahara, T. 1999. Cancer mortality among radiological technologists in Japan: Updated analysis of follow-up data from 1969 to 1993. J. Epidemiol., 9: 61-72.
80. Zakeri, F., and, Assaei, R. G. 2004. Cytogenetic monitoring of personnel working in angiocardiography laboratories in Iran hospitals. Mutat. Res., 562: 1-9.
81. Zhu, C. Q., Lam, T. H., Jiang, C. Q., Wei, B. X., Lou, X., Liu, W. W., Lao, X. Q., and, Chen, Y. H. 1999. Lymphocyte DNA damage in cigarette factory workers measured by the comet assay. Mutat. Res., 444: 1-6.
82. Zwingmann, I. H., Welle, I. J., Van Herwijnen, M., Engelen, J. J. M., Schilderman, P. A. E. L., Smid, T., and, Kleijans, J. C. S. 1998. Oxidative DNA damage and cytogenetic effects in flight engineers exposed to cosmic radiation. Environ. Mol. Mutagen., 32: 121-29.