Lack of adverse effect of smoking habit on DNA strand breakage and base damage, as revealed by the alkaline comet assay

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 440, Issue 1, 1999, Pages 19-25

  Wojewódzka, Maria ^a   Kruszewski, Marcin ^a   Iwanenko, Teresa ^a   Collins, Andrew R ^b   Szumiel, Irena ^a  

^a Inst Nucl Chem T   (Poland)

^b ROWETT RESEARCH INSTITUTE   (United Kingdom)

Author keywords

Comet assay; Human mononuclear blood cell; Smoking habit

Indexed keywords

ARTICLE; CARCINOGENESIS; CLINICAL ARTICLE; DNA DAMAGE; DNA STRAND BREAKAGE; GENOTOXICITY; HUMAN; METHODOLOGY; MONONUCLEAR CELL; NORMAL HUMAN; PRIORITY JOURNAL; SMOKING; SMOKING HABIT;

DNA; DNA DAMAGE; ELECTROPHORESIS, AGAR GEL; ENDODEOXYRIBONUCLEASES; FEMALE; HUMANS; LEUKOCYTES, MONONUCLEAR; MALE; MUTAGENICITY TESTS; SMOKING;

EID: 0345130049     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1383-5718(99)00014-5     Document Type: Article

References (35)

1. Wojewódzka M., Kruszewski M., Iwanenko T., Collins A.R., Szumiel I. Application of the comet assay for monitoring DNA damage in workers exposed to chronic low dose irradiation: I. Strand breakage. Mutat. Res. 416:1998;21-35.
2. Kruszewski M., Wojewódzka M., Iwanenko T., Collins A.R., Szumiel I. Application of the comet assay for monitoring DNA damage in workers exposed to chronic low dose irradiation: II. Base damage. Mutat. Res. 416:1998;37-57.
3. Frenzilli G., Betti C., Davini T., Desideri M., Fornai E., Giannessi L., Maggiorelli F., Paoletti P., Barale R. Evaluation of DNA damage in leukocytes of ex-smokers by single cell gel electrophoresis. Mutat. Res. 375:1997;117-123.
4. Rojas E., Valverde M., Sordo M., Ostrosky-Wegman P. DNA damage in exfoliated buccal cells of smokers assessed by the single cell electrophoresis assay. Mutat. Res. 370:1996;115-120.
5. Sardas S., Walker D., Akyol D., Karakaya A.E. Assessment of smoking-induced DNA damage in lymphocytes of smoking mothers of newborn infants using the alkaline single-cell gel electrophoresis technique. Mutat. Res. 335:1995;213-217.
6. Betti C., Davini T., Giannessi L., Loprieno N., Barale R. Microgel electrophoresis assay (comet test) and SCE analysis in human lymphocytes from 100 normal subjects. Mutat. Res. 307:1994;323-333.
7. Betti C., Davini T., Giannessi L., Loprieno N., Barale R. Comparative studies by comet test and SCE analysis in human lymphocytes from 200 healthy subjects. Mutat. Res. 343:1995;201-207.
8. Collins A.R., Dobson V.L., Dušinská M., Kennedy G., Štetina R. The comet assay: what can it really tell us? Mutat. Res. 375:1997;183-193.
9. Schantz S.P., Ostroff J.S. Novel approaches to the prevention of head and neck cancer. Proc. Soc. Exp. Biol. Med. 216:1997;275-282.
10. Scherer G., Richter E. Biomonitoring exposure to environmental tobacco smoke (ETS): a critical reappraisal. Human Exp. Toxicol. 16:1997;449-459.
11. Pryor W.A. Cigarette smoke radicals and the role of free radicals in chemical carcinogenicity. Environ. Health Perspect. 105:1997;875-882. Suppl 4.
12. Singh N.P., McCoy M.T., Tice R.R., Schneider E.L. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 175:1988;184-191.
13. Kruszewski M., Green M.H., Lowe J.E., Szumiel I. DNA strand breakage, cytotoxicity and mutagenicity of hydrogen peroxide treatment at 4°C and 37°C in L5178Y sublines. Mutat. Res. 308:1994;233-241.
14. Collins A.R., Duthie S.J., Dobson V.L. Direct enzymic detection of endogenous oxidative base damage in human lymphocyte DNA. Carcinogenesis. 14:1993;1733-1735.
15. J.H. Zar, Biostatistical Analysis. Prentice-Hall, Englewood Cliffs, NJ, 1984.
16. Romano G., Mancini R., Fedeele P., Curigliano G., Flamini G., Giovagnoli M.R., Malara N., Boninsegna A., Vecchione A., Santella R.M., Cittadini A. Immunohistochemical analysis of 4-aminobiphenyl-DNA adducts in oral mucosal cells of smokers and non-smokers. Anticancer Res. 17:1997;2827-2830.
17. Asami S., Hirano T., Yamaguchi R., Tomioka Y., Itoh H., Kasai H. Increase of a type of oxidative DNA damage, 8-hydroxyguanine, and its repair activity in human leukocytes by cigarette smoking. Cancer Res. 56:1996;2546-2549.
18. Shen H.M., Chia S.E., Ni Z.Y., New A.L., Lee B.L., Ong C.N. Detection of oxidative DNA damage in human sperm and the association with cigarette smoking. Reprod. Toxicol. 11:1997;675-680.
19. Holz O., Meissner R., Einhaus M., Koops F., Warncke K., Scherer G., Adlkofer F., Baumgartner E., Rudiger H.W. Detection of DNA single-strand breaks in lymphocytes of smokers. Int. Arch. Occupat. Environ. Health. 65:1993;83-88.
20. Asami S., Manabe H., Miyake J., Tsurudome Y., Hirano T., Yamaguchi R., Itoh H., Kasai H. Cigarette smoking induces an increase in oxidative DNA damage, 8-hydroxydeoxyguanosine, in a central site of the human lung. Carcinogenesis. 18:1997;1763-1766.
21. Reitz M., Coen R., Lanz E. DNA single-strand breaks in peripheral lymphocytes of clinical personnel with occupational exposure to volatile inhalational anesthetics. Environ. Res. 65:1994;12-21.
22. Stone J.G., Jones N.J., McGregor A.D., Waters R. Development of a human biomonitoring assay using buccal mucosa: comparison of smoking-related DNA adducts in mucosa versus biopsies. Cancer Res. 55:1995;1267-1270.
23. Phillips D.H. DNA adducts in human tissues: biomarkers of exposure to carcinogens in tobacco smoke. Environ. Health Perspect. 104:1996;453-458. Suppl 3.
24. Tates A.D., van Dam F.J., de Zwart F.A., Darroudi F., Natarajan A.T., Rossner P., Peterkova K., Peltonen K., Demopoulos N.A., Stephanou G., Vlachodimitropoulos D., Šram R.J. Biological effect monitoring in industrial workers from the Czech Republic exposed to low levels of butadiene. Toxicology. 113:1996;91-99.
25. Gedik C.M., Ewen S.W.B., Collins A.R. Single cell gel electrophoresis applied to the analysis of UV-C damage and its repair in human cells. Int. J. Radiat. Biol. 62:1992;313-320.
26. Billen D. Spontaneous DNA damage and its significance for the 'negligible dose' controversy in radiation protection. Radiat. Res. 124:1990;242-245.
27. Plappert U., Raddatz K., Roth S., Fliedner T.M. DNA-damage detection in man after radiation exposure - the comet assay - its possible application for human biomonitoring. Stem Cells. 13:1995;215-222. Suppl 1.
28. Banath J.P., Fushiki M., Olive P.L. Rejoining of DNA single- and double-strand breaks in human white blood cells exposed to ionizing radiation. Int. J. Radiat. Biol. 73:1998;649-660.
29. Oesch F., Hengstler J.G., Fuchs J. Cigarette smoking protects mononuclear blood cells of carcinogen exposed workers from additional work exposure-induced DNA single strand breaks. Mutat. Res. 321:1994;175-185.
30. Palus J., Dziubaltowska E., Rydzynski K. DNA single-strand breaks and DNA repair in the lymphocytes of wooden furniture workers. Mutat. Res. 408:1998;91-101.
31. Bolognesi C., Merlo F., Rabboni R., Valerio F., Abbondandolo A. Cytogenetic biomonitoring in traffic police workers, micronucleus test in peripheral blood lymphocytes. Environ. Mol. Mutagen. 30:1997;396-402.
32. Doerjer G., Buchholz U., Kreuzerand K., Oesch F. Biomonitoring of DNA damage by alkaline filter elution. Int. Arch. Occupat. Environ. Health. 60:1988;169-174.
33. Andreoli C., Leopardi P., Crebelli R. Detection of DNA damage in human lymphocytes by alkaline single cell gel electrophoresis after exposure to benzene or benzene metabolites. Mutat. Res. 377:1997;95-104.
34. Szumiel I. Monitoring and signaling of radiation damage in mammalian cells. Radiat. Res. 150:1998;S92-S101. Suppl.
35. Au W.W., Walker D.M., Ward J.B. Jr., Whorton E., Legator M.S., Singh V. Factors contributing to chromosome damage in lymphocytes of cigarette smokers. Mutat. Res. 260:1991;137-144.