Rapid detection methods for Bacillus anthracis in environmental samples: A review

Applied Microbiology and Biotechnology

Volumn 93, Issue 4, 2012, Pages 1411-1422

  Irenge, Léonid M ^a,b   Gala, Jean Luc ^a,b  

^a Defence Laboratories Department   (Belgium)

^b UNIVERSITÉ CATHOLIQUE DE LOUVAIN   (Belgium)

Author keywords

Affinity based methods; Bacillus anthracis; Culture; Environmental samples; Molecular; Rapid detection

Indexed keywords

AFFINITY-BASED METHODS; BACILLUS ANTHRACIS; ENVIRONMENTAL SAMPLE; MOLECULAR; RAPID DETECTION;

ANIMALS; BACTERIOLOGY; CELL CULTURE; DISEASES;

BACTERIA;

NUCLEIC ACID;

ANTHRAX; BACTERIAL DISEASE; BACTERIUM; BIOTERRORISM; DETECTION METHOD; DISEASE INCIDENCE; ENVIRONMENTAL ASSESSMENT; LITERATURE REVIEW; MOLECULAR ANALYSIS; NINETEENTH CENTURY; PATHOGEN; PUBLIC HEALTH;

BACILLUS ANTHRACIS; BACTERIUM DETECTION; ENZYME LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; IMMUNOASSAY; MAGNETIC PARTICLE FLUOROGENIC IMMUNOASSAY; RESONANCE ENERGY TRANSFER FLOW CYTOMETRY; SHORT SURVEY;

BACILLUS ANTHRACIS; BACTERIOLOGICAL TECHNIQUES; BIOLOGICAL WARFARE AGENTS; ENVIRONMENTAL MICROBIOLOGY; MOLECULAR DIAGNOSTIC TECHNIQUES; POINT-OF-CARE SYSTEMS;

UNITED STATES;

ANIMALIA; BACILLUS (BACTERIUM); BACILLUS ANTHRACIS; BACTERIA (MICROORGANISMS); POSIBACTERIA;

EID: 84857924846     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3845-7     Document Type: Short Survey

References (153)

1. Agaisse H, Gominet M, Okstad OA, Kolstø AB, Lereclus D (1999) PlcR is a pleiotropic regulator of extracellular virulence factor gene expression in Bacillus thuringiensis. Mol Microbiol 32:1043-1053 (Pubitemid 29255770)
2. Antwerpen MH, Zimmermann P, Bewley K, Frangoulidis D, Meyer H (2008) Real-time PCR system targeting a chromosomal marker specific for Bacillus anthracis. Mol Cell Probes 22:313-315
3. AOAC International (2004) Initiative yields effective methods for anthrax detection; RAMP and MIDI, Inc., methods approved. Inside Lab. Management 10:3
4. Ash C, Collins MD (1992) Comparative analysis of 23S ribosomal RNA gene sequences of Bacillus anthracis and emetic Bacillus cereus determined by PCR-direct sequencing. FEMS Microbiol Lett 73:75-80
5. Ash C, Farrow JA, Dorsch M, Stackebrandt E, Collins MD (1991) Comparative analysis of Bacillus anthracis, Bacillus cereus, related species on the basis of reverse transcriptase sequencing of 16S rRNA. Int J Syst Bacteriol 41:343-346
6. Baillie LW, Jones MN, Turnbull PC, Manchee RJ (1995) Evaluation of the Biolog system for the identification of Bacillus anthracis. Lett Appl Microbiol 20:209-211
7. Barns SM, Grow CC, Okinaka RT, Keim P, Kuske CR (2005) Detection of diverse new Francisella-like bacteria in environmental samples. Appl Environ Microbiol 71:5494-5500 (Pubitemid 41338285)
8. Beyer W, Glöckner P, Otto J, Böhm R (1995) A nested PCR method for the detection of Bacillus anthracis in environmental samples collected from former tannery sites. Microbiol Res 150:179-186
9. Biagini RE, Sammons DL, Smith JP, MacKenzie BA, Striley CA, Robertson SA, Snawder JE, Quinn CP (2005) Simultaneous measurement of specific serum IgG responses to five select agents. Anal Bioanal Chem 382:1027-1034 (Pubitemid 40890962)
10. Biagini RE, Sammons DL, Smith JP, MacKenzie BA, Striley CA, Semenova V, Steward-Clark E, Stamey K, Freeman AE, Quinn CP, Snawder JE (2004) Comparison of a multiplexed fluorescent covalent microsphere immunoassay an enzyme-linked immunosorbent assay for measurement of human immunoglobulin G antibodies to anthrax toxins. Clin Diagn Lab Immunol 11:50-55 (Pubitemid 38090651)
11. Blackwood KS, Turenne CY, Harmsen D, Kabani AM (2004) Reassessment of sequence-based targets for identification of bacillus species. J Clin Microbiol 42:1626-1630 (Pubitemid 38500807)
12. Boyer AE, Gallegos-Candela M, Lins RC, Kuklenyik Z, Woolfitt A, Moura H, Kalb S, Quinn CP, Barr JR (2011) Quantitative mass spectrometry for bacterial protein toxins - a sensitive, specific, high-throughput tool for detection and diagnosis. Molecules 16:2391-2413
13. Brahmbatt TN, Darnell SC, Carvalho HM, Sanz P, Kang TJ, Bukk RL, Rasmussen SB, Cross AS, O'Brien AD (2007) Recombinant exosporium protein BclA of Bacillus anthracis is effective as a booster for mice primed with suboptimal amounts of protective antigen. Infect Immun 75:5240-5247 (Pubitemid 350033610)
14. Brigati J, Williams DD, Sorokulova IB, Nanduri V, Chen IH, Turnbough CL Jr, Petrenko VA (2004) Diagnostic probes for Bacillus anthracis spores selected from a landscape phage library. Clin Chem50:1899-1906 (Pubitemid 39298034)
15. Brossier F, Levy M, Mock M (2002) Anthrax spores make an essential contribution to vaccine efficacy. Infect Immun 70:661-664 (Pubitemid 34083825)
16. Bruno JG, Kiel JL (1999) In vitro selection of DNA aptamers to anthrax spores with electrochemiluminescence detection. Biosens Bioelectron 14:457-464 (Pubitemid 29333570)
17. Bruno JG, Yu H (1996) Immunomagnetic-electrochemiluminescent detection of Bacillus anthracis spores in soil matrices. Appl Environ Microbiol 62:3474-3476 (Pubitemid 26304145)
18. Burton JE, Oshota OJ, North E, Hudson MJ, Polyanskaya N, Brehm J, Lloyd G, Silman NJ (2005) Development of a multi-pathogen oligonucleotide microarray for detection of Bacillus anthracis. Mol Cell Probes 19:349-357 (Pubitemid 41282801)
19. Burton JE, Oshota OJ, Silman NJ (2006) Differential identification of Bacillus anthracis from environmental Bacillus species using microarray analysis. J Appl Microbiol 101:754-763 (Pubitemid 44392899)
20. Campbell GA, Mutharasan R (2006) Piezoelectric-excited millimetersized cantilever (PEMC) sensors detect Bacillus anthracis at 300 spores/mL. Biosens Bioelectron 21:1684-1692 (Pubitemid 43254757)
21. CDC (2006). Bioterrorism Overview, at www.bt.cdc.gov/agent/agentlist.asp
22. Charlton S, Moir AJ, Baillie L, Moir A (1999) Characterization of the exosporium of Bacillus cereus. J Appl Microbiol 87:241-245 (Pubitemid 29430334)
23. Cherif A, Borin S, Rizzi A, Ouzari H, Boudabous A, Daffonchio D (2002) Characterization of a repetitive element polymorphismpolymerase chain reaction chromosomal marker that discriminates Bacillus anthracis from related species. J Appl Microbiol 93:456-462 (Pubitemid 36002450)
24. Cheun HI, Makino SI, Watarai M, Erdenebaatar J, Kawamoto K, Uchida I (2003) Rapid effective detection of anthrax spores in soil by PCR. J Appl Microbiol 95:728-733 (Pubitemid 37186655)
25. Choi JS, Kim SG, Lahousse M, Park HY, Park HC, Jeong B, Kim J, Kim SK, Yoon MY (2011) Screening and characterization of high-affinity ssDNA aptamers against anthrax protective antigen. J Biomol Screen 16:266-271
26. Cohen S, Mendelson I, Altboum Z, Kobiler D, Elhanany E, Bino T, Leitner M, Inbar I, Rosenberg H, Gozes Y, Barak R, Fisher M, Kronman C, Velan B, Shafferman A (2000) Attenuated nontoxinogenic and nonencapsulated recombinant Bacillus anthracis spore vaccines protect against anthrax. Infect Immun 68:4549-4558 (Pubitemid 30497722)
27. Cummings CA, Bormann Chung CA, Fang R, Barker M, Brzoska P, Williamson PC, Beaudry J, Matthews M, Schupp J, Wagner DM, Birdsell D, Vogler AJ, Furtado MR, Keim P, Budowle B (2010) Accurate, rapid and high-throughput detection of strain-specific polymorphisms in Bacillus anthracis and Yersinia pestis by next-generation sequencing. Investig Genets 2010(1):5
28. Daffonchio D, Borin S, Frova G, Gallo R, Mori E, Fani R, Sorlini C (1999) A randomly amplified polymorphic DNA marker specific for the Bacillus cereus group is diagnostic for Bacillus anthracis. Appl Environ Microbiol 65:1298-1303
29. Daffonchio D, Cherif A, Borin S (2000) Homoduplex and heteroduplex polymorphisms of the amplified ribosomal 16S-23S internal transcribed spacers describe genetic relationships in the "Bacillus cereus group". Appl Environ Microbiol 66:5460-5468 (Pubitemid 32217156)
30. Dang JL, Heroux K, Kearny J, Arasteh A, Gostomski M, Emanuel PA (2001) Bacillus spore inactivation affect detection assays. Appl Environ Microbiol 67:3665-3670 (Pubitemid 33641240)
31. Daubenspeck JM, Zeng H, Chen P, Dong S, Steichen CT, Krishna NR, Pritchard DG, Turnbough CL Jr (2004) Novel oligosaccharide side chains of the collagen-like region of BclA, the major glycoprotein of the Bacillus anthracis exosporium. J Biol Chem 279:30945-30953 (Pubitemid 39037753)
32. Davila AP, Jang J, Gupta AK, Walter T, Aronson A, Bashir R (2007) Microresonator mass sensors for detection of Bacillus anthracis Sterne spores in air and water. Biosens Bioelectron 22:3028-3035 (Pubitemid 46778822)
33. Dixon TC, Fadl AA, Koehler TM, Swanson JA, Hanna PC (2000) Early Bacillus anthracis-macrophage interactions: intracellular survival survival and escape. Cell Microbiol 2:453-463 (Pubitemid 32049317)
34. Dixon TC, Meselson M, Guillemin J, Hanna PC (1999) Anthrax. N Engl J Med 341:815-826 (Pubitemid 29420478)
35. Dua P, Kim S, Lee DK (2011) Nucleic acid aptamers targeting cellsurface proteins. Methods 54:215-25
36. Easterday WR, Van Ert MN, Zanecki S, Keim P (2005) Specific detection of Bacillus anthracis using a TaqMan (R) mismatch amplification mutation assay. BioTechniques 38:731-735 (Pubitemid 40647042)
37. Ellington AD, Szostak JW (1990) In vitro Selection of RNA molecules that bind specific ligands. Nature 346:818-822
38. Elsaghir AA, James EA (2003) Misidentification of Brucella melitensis as Ochrobactrum anthropi by API 20NE. J Med Microbiol 52:441-442 (Pubitemid 36589172)
39. Ezzell JW, Welkos SL (1999) The capsule of Bacillus anthracis, a review. J Appl Microbiol 87:250
40. Faulhammer D, Eschgfäller B, Stark S, Burgstaller P, Englberger W, Erfurth J, Kleinjung F, Rupp J, Dan Vulcu S, Schröder W, Vonhoff S, Nawrath H, Gillen C, Klussmann S (2004) Biostable aptamers with antagonistic properties to the neuropeptide nociceptin/orphanin FQ. RNA 10:516-527 (Pubitemid 38241011)
41. Fenton M, Ross P, McAuliffe O, O'Mahony J, Coffey A (2010) Recombinant bacteriophage lysins as antibacterials. Bioeng Bugs 1:9-16
42. Fujinami Y, Hirai Y SI, Yoshino M, Yasuda J (2007) Sensitive detection of Bacillus anthracis using a binding protein originating from gamma-phage. Microbiol Immunol 51:163-169
43. Garcia-Patrone M, Tandecarz JS (1995) A glycoprotein multimer from Bacillus thuringiensis sporangia: dissociation into subunits and sugar composition. Mol Cell Biochem 145:29-37
44. Guglielmo-Viret V, Vietri NJ, Desor F, Thullier P (2011) Combination of cultivation and lateral flow assay for easy, highly sensitive and presumptive detection of Bacillus anthracis spores. J Med Lab Diagn 2:51-53
45. Guichard A, Nizet V, Bier E (2011) New insights into the biological effects of anthrax toxins: linking cellular to organismal responses. Microbes Infect. doi:10.1016/j.micinf.2011.08.016
46. Hang J, Sundaram AK, Zhu P, Shelton DR, Karns JS, Martin PA, Li S, Amstutz P, Tang CM (2008) Development of a rapid and sensitive immunoassay for detection and subsequent recovery of Bacillus anthracis spores in environmental samples. J Microbiol Methods 73:242-246
47. Hao RZ, Song HB, Zuo GM, Yang RF, Wei HP, Wang DB, Cui ZQ, Zhang Z, Cheng ZX, Zhang XE (2011) DNA probe functionalized QCM biosensor based on gold nanoparticle amplification for Bacillus anthracis detection. Biosens Bioelectron 26:3398-3404
48. Hao R, Wang D, Zhang X, Zuo G, Wei H, Yang R, Zhang Z, Cheng Z, Guo Y, Cui Z, Zhou Y (2009) Rapid detection of Bacillus anthracis using monoclonal antibody functionalized QCM sensor. Biosens Bioelectron 24:1330-1335
49. Harrell LJ, Andersen GL, Wilson KH (1995) Genetic variability of Bacillus anthracis and related species. J Clin Microbiol 33:1847-1850
50. Hatano B, Maki T, Obara T, Fukumoto H, Hagisawa K, Matsushita Y, Okutani A, Bazartseren B, Inoue S, Sata T, Katano H (2010) LAMP using a disposable pocket warmer for anthrax detection, a highly mobile and reliable method for anti-bioterrorism. Jpn J Infect Dis 63:36-40
51. Helgason E, Okstad OA, Caugant DA, Johansen HA, Fouet A, Mock M, Hegna I, Kolstø AB (2000) Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis-one species on the basis of genetic evidence. Appl Environ Microbiol 66:2627-2630 (Pubitemid 30353974)
52. Helgason E, Tourasse NJ, Meisal R, Caugant DA, Kolstø AB (2004) Multilocus sequence typing scheme for bacteria of the Bacillus cereus group. Appl Environ Microbiol 70:191-201 (Pubitemid 38090204)
53. Herzog AB, McLennan SD, Pandey AK, Gerba CP, Haas CN, Rose JB, Hashsham SA (2009) Implications of limits of detection of various methods for Bacillus anthracis in computing risks to human health. Appl Environ Microbiol 75:6331-6339
54. Hindson BJ, McBride MT, Makarewicz AJ, Henderer BD, Setlur US, Smith SM, Gutierrez DM, Metz TR, Nasarabadi SL, Venkateswaran KS, Farrow SW, Colston BW Jr, Dzenitis JM (2005) Autonomous detection of aerosolized biological agents by multiplexed immunoassay with polymerase chain reaction confirmation. Anal Chem 77:284-289 (Pubitemid 40096697)
55. Hoffmaster AR, Ravel J, Rasko DA, Chapman GD, Chute MD, Marston CK, De BK, Sacchi CT, Fitzgerald C, Mayer LW, Maiden MC, Priest FG, Barker M, Jiang L, Cer RZ, Rilstone J, Peterson SN, Weyant RS, Galloway DR, Read TD, Popovic T, Fraser CM (2004) Identification of anthrax toxin genes in a Bacillus cereus associated with an illness resembling inhalation anthrax. Proc Natl Acad Sci U S A 101:8449-8454 (Pubitemid 38736595)
56. Hoffmaster AR, Hill KK, Gee JE, Marston CK, De BK, Popovic T, Sue D, Wilkins PP, Avashia SB, Drumgoole R, Helma CH, Ticknor LO, Okinaka RT, Jackson PJ (2006) Characterization of Bacillus cereus isolates associated with fatal pneumonias: strains are closely related to Bacillus anthracis and harbor B. anthracis virulence genes. J Clin Microbiol 44:3352-3360 (Pubitemid 44413776)
57. Hu X, Swiecicka I, Timmery S, Mahillon J (2009) Sympatric soil communities of Bacillus cereus sensu lato: population structure potential plasmid dynamics of pXO1- and pXO2-like elements. FEMS Microbiol Ecol 70:344-355
58. Huang Z, Pei W, Jayaseelan S, Shi H, Niu L (2007) RNA aptamers selected against the GluR2 glutamate receptor channel. Biochemistry 46:12648-12655 (Pubitemid 350060089)
59. Hurtle W, Bode E, Kulesh DA, Kaplan RS, Garrison J, Bridge D, House M, Frye MS, Loveless B, Norwood D (2004) Detection of the Bacillus anthracis gyrA gene by using a minor groove binder probe. J Clin Microbiol 42:179-185 (Pubitemid 38082717)
60. Inglesby TV, Henderson DA, Bartlett JG, Ascher MS, Eitzen E, Friedlander AM, Hauer J, McDade J, Osterholm MT, O'Toole T, Parker G, Perl TM, Russell PK, Tonat K (1999) Anthrax as a biological weapon: medical and public health management. Working Group on Civilian Biodefense. JAMA 281:1735-1745 (Pubitemid 29226485)
61. Irenge LM, Durant JF, Tomaso H, Pilo P, Olsen JS, Ramisse V, Mahillon J, Gala JL (2010) Development and validation of a real-time quantitative PCR assay for rapid identification of Bacillus anthracis in environmental samples. Appl Microbiol Biotechnol 88:1179-1192
62. Jackson PJ, Walthers EA, Kalif AS, Richmond KL, Adair DM, Hill KK, Kuske CR, Andersen GL, Wilson KH, Hugh-Jones M, Keim P (1997) Characterization of the variable-number tandem repeats in vrrA from different Bacillus anthracis isolates. Appl Microbiol Biotechnol 63:1400-1405 (Pubitemid 27174847)
63. Jeong S, Lee HK, Kim MY (2009) Use of RNA aptamers for the modulation of cancer cell signaling. Methods Mol Biol 542:363-377
64. Jernigan DB, Raghunathan PL, Bell BP, Brechner R, Bresnitz EA, Butler JC, Cetron M, Cohen M, Doyle T, Fischer M, Greene C, Griffith KS, Guarner J, Hadler JL, Hayslett JA, Meyer R, Petersen LR, Phillips M, Pinner R, Popovic T, Quinn CP, Reefhuis J, Reissman D, Rosenstein N, Schuchat A, Shieh WJ, Siegal L, Swerdlow DL, Tenover FC, Traeger M, Ward JW, Weisfuse I, Wiersma S, Yeskey K, Zaki S, Ashford DA, Perkins BA, Ostroff S, Hughes J, Fleming D, Koplan JP, Gerberding JL, Team National Anthrax Epidemiologic Investigation (2002) Investigation of bioterrorism-related anthrax, United States, 2001: epidemiologic findings. Emerg Infect Dis 8:1019-1028 (Pubitemid 35256987)
65. Keim P, Kalif A, Schupp J, Hill K, Travis SE, Richmond K, Adair DM, Hugh-Jones M, Kuske CR, Jackson P (1997) Molecular evolution and diversity in Bacillus anthracis as detected by amplified fragment length polymorphism markers. J Bacteriol 179:818-824 (Pubitemid 27051838)
66. Keim P, Price LB, Klevytska AM, Smith KL, Schupp JM, Okinaka R, Jackson PJ, Hugh-Jones ME (2000) Multiple-locus variable-number tandem repeat analysis reveals genetic relationships within Bacillus anthracis. J Bacteriol 182:2928-2936 (Pubitemid 30246926)
67. Keim P, Gruendike JM, Klevytska AM, Schupp JM, Challacombe J, Okinaka R (2009) The genome and variation of Bacillus anthracis. Mol Aspects Med 30:397-405
68. Keys CJ, Dare DJ, Sutton H, Wells G, Lunt M, McKenna T, McDowall M, Shah HN (2004) Compilation of a MALDI-TOF mass spectral database for the rapid screening and characterization of bacteria implicated in human infectious diseases. Infect Genet Evol 4:221-242 (Pubitemid 39185928)
69. Kikkawa H, Fujinami Y, Suzuki S, Yasuda J (2007) Identification of the amino acid residues critical for specific binding of the bacteriolytic enzyme of gamma-phage, PlyG, to Bacillus anthracis. Biochem Biophys Res Commun 363:531-535 (Pubitemid 47513663)
70. Kikkawa HS, Ueda T, Suzuk S, Yasuda J (2008) Characterization of the catalytic activity of the gamma-phage lysin, PlyG, specific for Bacillus anthracis. FEMS Microbiol Lett 286:236-240
71. Kim J, Yoon MY (2010) Recent advances in rapid and ultrasensitive biosensors for infectious agents: lesson from Bacillus anthracis diagnostic sensors. Analyst 135:1182-1190
72. King D, Luna V, Cannons A, Cattani J, Amuso P (2003) Performance assessment of three commercial assays for direct detection of Bacillus anthracis spores. J Clin Microbiol 41:3454-3455 (Pubitemid 36842379)
73. Klee SR, Nattermann H, Becker S, Urban-Schriefer M, Franz T, Jacob D, Appel B (2006a) Evaluation of different methods to discriminate Bacillus anthracis from other bacteria of the Bacillus cereus group. J Appl Microbiol 100:673-681
74. Klee SR, Ozel M, Appel B, Boesch C, Ellerbrok H, Jacob D, Holland G, Leendertz FH, Pauli G, Grunow R, Nattermann H (2006b) Characterization of Bacillus anthracis-like bacteria isolated from wild great apes from Cote d'Ivoire and Cameroon. J Bacteriol 188:5333-5344 (Pubitemid 44157289)
75. Klee SR, Brzuszkiewicz EB, Nattermann H, Brüggemann H, Dupke S, Wollherr A, Franz T, Pauli G, Appel B, Liebl W, Couacy-Hymann E, Boesch C, Meyer FD, Leendertz FH, Ellerbrok H, Gottschalk G, Grunow R, Liesegang H (2010) The genome of a Bacillus isolate causing anthrax in chimpanzees combines chromosomal properties of B. cereus with B. anthracis virulence plasmids. PLoS One 5:e10986
76. Klichko VI, Miller J, Wu A, Popov SG, Alibek K (2003) Anaerobic induction of Bacillus anthracis hemolytic activity. Biochem Biophys Res Commun 303:855-862 (Pubitemid 36384933)
77. Knisely RF (1966) Selective medium for Bacillus anthracis. J Bacteriol 92:784-786
78. Ko KS, Kim JM, Kim JW, Jung BY, Kim W, Kim IJ, Kook YH (2003) Identification of Bacillus anthracis by rpoB sequence analysis and multiplex PCR. J Clin Microbiol 41:2908-2914 (Pubitemid 36842278)
79. Kuehn A, Kovác P, Saksena R, Bannert N, Klee SR, Ranisch H, Grunow R (2009) Development of antibodies against anthrose tetrasaccharide for specific detection of Bacillus anthracis spores. Clin Vaccine Immunol 16:1728-1737
80. Kurosaki Y, Sakuma T, Fukuma A, Fujinami Y, Kawamoto K, Kamo N, Makino SI, Yasuda J (2009) A simple and sensitive method for detection of Bacillus anthracis by loop-mediated isothermal amplification. J Appl Microbiol 107:1947-1956
81. Kuske CR, Barns SM, Grow CC, Merrill L, Dunbar J (2006) Environmental survey for four pathogenic bacteria and closely related species using phylogenetic and functional genes. J Forensic Sci 51:548-558
82. Lasch P, Beyer W, Nattermann H, Stämmler M, Siegbrecht E, Grunow R, Naumann D (2009) Identification of Bacillus anthracis by using matrix-assisted laser desorption ionization-time of flight mass spectrometry and artificial neural networks. Appl Environ Microbiol 75:7229-7242
83. Léonard C, Zekri O, Mahillon J (1998) Integrated physical and genetic mapping of Bacillus cereus and other gram-positive bacteria based on IS231A transposition vectors. Infect Immun 66:2163-2169 (Pubitemid 28194116)
84. Le Flèche P, Hauck Y, Onteniente L, Prieur A, Denoeud F, Ramisse V, Sylvestre P, Benson G, Ramisse F, Vergnaud G (2001) A tandem repeats database for bacterial genomes: application to the genotyping of Yersinia pestis and Bacillus anthracis. BMC Microbiol 1:2
85. Leski TA, Caswell CC, Pawlowski M, Klinke DJ, Bujnicki JM, Hart SJ, Lukomski S (2009) Identification and classification of bcl genes and proteins of Bacillus cereus group organisms and their application in Bacillus anthracis detection and fingerprinting. Appl Environ Microbiol 75:7163-7172
86. Lim DV (2003) Detection of microorganisms and toxins with evanescent wave fiber-optic biosensors. Proceedings of the Ieee 91:902-907
87. Lim DV, Simpson JM, Kearns EA, Kramer MF (2005) Current and developing technologies for monitoring agents of bioterrorism and biowarfare. Clin Microbiol Rev 18:583-607 (Pubitemid 41504507)
88. Lim YC, Kouzani AZ, Duan W (2010) Aptasensors: a review. J Biomed Nanotechnol 6:93-105
89. Lindstedt BA (2005) Multiple-locus variable number tandem repeats analysis for genetic fingerprinting of pathogenic bacteria. Electrophoresis 26:2567-2582
90. Lista F, Faggioni G, Valjevac S, Ciammaruconi A, Vaissaire J, le Doujet C, Gorgé O, De Santis R, Carattoli A, Ciervo A, Fasanella A, Orsini F, D'Amelio R, Pourcel C, Cassone A, Vergnaud G (2006) Genotyping of Bacillus anthracis strains based on automated capillary 25-loci multiple locus variable-number tandem repeats analysis. BMC Microbiol 6:33
91. Makino SI, Iinuma-Okada Y, Maruyama T, Ezaki T, Sasakawa C, Yoshikawa M (1993) Direct detection of Bacillus anthracis DNA in animals by polymerase chain reaction. J Clin Microbiol 31:547-551 (Pubitemid 23059864)
92. Marston CK, Gee JE, Popovic T, Hoffmaster AR (2006) Molecular approaches to identify and differentiate Bacillus anthracis from phenotypically similar Bacillus species isolates.". BMC Microbiol 6:22
93. Mehta AS, Saile E, Zhong W, Buskas T, Carlson R, Kannenberg E, Reed Y, Quinn CP, Boons GJ (2006) Synthesis and antigenic analysis of the BclA glycoprotein oligosaccharide from the Bacillus anthracis exosporium. Chemistry 12:9136-9149 (Pubitemid 46008502)
94. Merrill L, Dunbar J, Richardson J, Kuske CR (2006) Composition of Bacillus species in aerosols from 11 U.S. cities. J Forensic Sci 51:559-565
95. Mesnage S, Tosi-Couture E, Gounon P, Mock M, Fouet A (1998) The capsule and S-layer: two independent and yet compatible macromolecular structures in Bacillus anthracis. J Bacteriol 180:52-58 (Pubitemid 28023782)
96. Mesnage S, Tosi-Couture E, Mock M, Gounon P, Fouet A (1997) Molecular characterization of the Bacillus anthracis main Slayer component: evidence that it is the major cell-associated antigen. Mol Microbiol 23:1147-1155 (Pubitemid 27122200)
97. Mignot T, Mock M, Robichon D, Landier A, Lereclus D, Fouet A (2001) The incompatibility between the PlcR- and AtxAcontrolled regulons may have selected a nonsense mutation in Bacillus anthracis. Mol Microbiol 42:1189-1198
98. Mock M, Fouet A (2001) Anthrax. Annu Rev Microbiol 55:647-671 (Pubitemid 32978119)
99. Mock M, Mignot T (2003) Anthrax toxins and the host: a story of intimacy. Cell Microbiol 5:15-23 (Pubitemid 36395117)
100. Nayak M, Kotian A, Marathe S, Chakravortty D (2009) Detection of microorganisms using biosensors - a smarter way towards detection techniques. Biosens Bioelectron 25:661-667
101. Notomi T, Okayama H, Masubuchi H, YonekawaT WK, Amino N, Hase T (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28:E63
102. Oggioni MR, Meacci F, Carattoli A, Ciervo A, Orru G, Cassone A, Pozzi G (2002) Protocol for real-time PCR identification of anthrax spores from nasal swabs after broth enrichment. Clin Microbiol 40:3956-3963 (Pubitemid 35285773)
103. Olsen JS, Skogan G, Fykse EM, Rawlinson EL, Tomaso H, Granum PE, Blatny JM (2007) Genetic distribution of 295 Bacillus cereus group members based on adk-screening in combination with MLST (Multilocus Sequence Typing) used for validating a primer targeting a chromosomal locus in B. anthracis. J Microbiol Methods 71:265-274 (Pubitemid 350151528)
104. Pannucci J, Okinaka RT, Sabin R, Kuske CR (2002) Bacillus anthracis pXO1 plasmid sequence conservation among closely related bacterial species. J Bacteriol 184:134-141 (Pubitemid 34003293)
105. Patra G, Sylvestre P, Ramisse V, Thérasse J, Guesdon JL (1996) Isolation of a specific chromosomic DNA sequence of Bacillus anthracis and its possible use in diagnosis. FEMS Immunol Med Microbiol 15:223-231 (Pubitemid 26344652)
106. Patra G, Vaissaire J, Weber-Levy M, Le Doujet C, Mock M (1998) Molecular characterization of Bacillus strains involved in outbreaks of anthrax in France in 1997. J Clin Microbiol 36:3412-3414 (Pubitemid 28476209)
107. Petrenko VA, Vodyanoy VJ (2003) Phage display for detection of biological threat agents. J Microbiol Methods 53:253-262 (Pubitemid 36332359)
108. Priest FG, Barker M, Baillie LW, Holmes EC, Maiden MC (2004) Population structure and evolution of the Bacillus cereus group. J Bacteriol 186:7959-7970 (Pubitemid 39552507)
109. Qi Y, Patra G, Liang X, Williams LE, Rose S, Redkar RJ, DelVecchio VG (2001) Utilization of the rpoB gene as a specific chromosomal marker for real-time PCR detection of Bacillus anthracis. Appl Environ Microbiol 67:3720-3727 (Pubitemid 33641249)
110. Radnedge L, Agron PG, Hill KK, Jackson PJ, Ticknor LO, Keim P, Andersen GL (2003) Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Appl Environ Microbiol 69:2755-2764 (Pubitemid 36560354)
111. Ramisse V, Patra G, Vaissaire J, Mock M (1999) The Ba813 chromosomal DNA sequence effectively traces the whole Bacillus anthracis community. J Appl Microbiol 87:224-228 (Pubitemid 29430331)
112. Rao SS, Mohan KV, Atreya CD (2010) Detection technologies for Bacillus anthracis: prospects and challenges. J Microbiol Methods 82:1-10
113. Rasko DA, Rosovitz MJ, ∅kstad OA, Fouts DE, Jiang L, Cer RZ, Kolstø AB, Gill SR, Ravel J (2007) Complete sequence analysis of novel plasmids from emetic and periodontal Bacillus cereus isolates reveals a common evolutionary history among the B. cereus-group plasmids, including Bacillus anthracis pXO1. J Bacteriol 189:52-64 (Pubitemid 46036221)
114. Rasko DA, Ravel J, ∅kstad OA, Helgason E, Cer RZ, Jiang L, Shores KA, Fouts DE, Tourasse NJ, Angiuoli SV, Kolonay J, Nelson WC, Kolstø AB, Fraser CM, Read TD (2004) The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1. Nucleic Acids Res 32:977-988 (Pubitemid 38854698)
115. Rasko DA, Worsham PL, Abshire TG, Stanley ST, Bannan JD, Wilson MR, Langham RJ, Decker RS, Jiang L, Read TD, Phillippy AM, Salzberg SL, Pop M, Van Ert MN, Kenefic LJ, Keim PS, Fraser- Liggett CM, Ravel J (2011) Bacillus anthracis comparative genome analysis in support of the Amerithrax investigation. Proc Natl Acad Sci U S A 108:5027-5032
116. Read TD, Peterson SN, Tourasse N, Baillie LW, Paulsen IT, Nelson KE, Tettelin H, Fouts DE, Eisen JA, Gill SR, Holtzapple EK, Okstad OA, Helgason E, Rilstone J, Wu M, Kolonay JF, Beanan MJ, Dodson RJ, Brinkac LM, Gwinn M, DeBoy RT, Madpu R, Daugherty SC, Durkin AS, Haft DH, Nelson WC, Peterson JD, Pop M, Khouri HM, Radune D, Benton JL, Mahamoud Y, Jiang L, Hance IR, Weidman JF, Berry KJ, Plaut RD, Wolf AM, Watkins KL, Nierman WC, Hazen A, Cline R, Redmond C, Thwaite JE, White O, Salzberg SL, Thomason B, Friedlander AM, Koehler TM, Hanna PC, Kolstø AB, Fraser CM (2003) The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria. Nature 423:81-86 (Pubitemid 36569586)
117. Ryu C, Lee K, Yoo C, Seong WK, Oh HB (2003) Sensitive and rapid quantitative detection of anthrax spores isolated from soil samples by real-time PCR. Microbiol Immunol 47:693-699 (Pubitemid 37288245)
118. Sacchi CT, Whitney AM, Mayer LW, Morey R, Steigerwalt A, Boras A, Weyant RS, Popovic T (2002) Sequencing of 16S rRNA gene: a rapid tool for identification of Bacillus anthracis. Emerg Infect Dis 8:1117-1123 (Pubitemid 35257004)
119. Sainathrao S, Mohan KV, Atreya C (2009) Gamma-phage lysin PlyG sequence-based synthetic peptides coupled with Qdot-nanocrystals are useful for developing detection methods for Bacillus anthracis by using its surrogates, B. anthracis-Sterne and B. cereus-4342. BMC Biotechnol 9:67
120. Sastry KS, Tuteja U, Santhosh PK, Lalitha MK, Batra HV (2003) Identification of Bacillus anthracis by a simple protective antigen-specific mAb dot-ELISA. J Med Microbiol 52:47-49 (Pubitemid 36106368)
121. Scholz HC, Pfeffer M, Witte A, Neubauer H, Al Dahouk S, Wernery U, Tomaso H (2008) Specific detection and differentiation of Ochrobactrum anthropi, Ochrobactrum intermedium and Brucella spp. by a multi-primer PCR that targets the recA gene. J Med Microbiol 57:64-71
122. Song Y, Yang R, Guo Z, Zhang M, Wang X, Zhou F (2000) Distinctness of spore and vegetative cellular fatty acid profiles of some aerobic endospore-forming bacilli. J Microbiol Methods 109:1509-1520
123. Spencer RC (2003) Bacillus anthracis. J Clin Pathol 56:182-187
124. Stoltenburg R, Reinemann C, Strehlitz B (2007) SELEX-a (r)evolutionary method to generate high-affinity nucleic acid ligands. Biomol Eng 24:381-403 (Pubitemid 47391579)
125. Stopa PJ (2000) The flow cytometry of Bacillus anthracis spores revisited. Cytometry 41:237-244
126. Swanson ER, Fosnocht DE (2000) Anthrax threats: a report of two incidents from Salt Lake City. J Emerg Med 18:229-232 (Pubitemid 30105826)
127. Sylvestre P, Couture-Tosi E, Mock M (2002) A collagen-like surface glycoprotein is a structural component of the Bacillus anthracis exosporium. Mol Microbiol 45:169-178 (Pubitemid 35025942)
128. Taitt CR, Anderson GP, Lingerfelt BM, Feldstein MJ, Ligler FS (2002) Nine-analyte detection using an array-based biosensor. Anal Chem 74:6114-6120 (Pubitemid 35423921)
129. Tamborrini M, Holzer M, Seeberger PH, Schürch N, Pluschke G (2010) Anthrax spore detection by a luminex assay based on monoclonal antibodies that recognize anthrose-containing oligosaccharides. Clin Vaccine Immunol 17:1446-1451
130. Tamborrini M, Oberli MA, Werz DB, Schürch N, Frey J, Seeberger PH, Pluschke G (2009) Immuno-detection of anthrose containing tetrasaccharide in the exosporium of Bacillus anthracis and Bacillus cereus strains. J Appl Microbiol 106:1618-1628
131. Tamborrini M, Werz DB, Frey J, Pluschke G, Seeberger PH (2006) Anti-carbohydrate antibodies for the detection of anthrax spores. Angew Chem Int Ed Engl 45:6581-6582 (Pubitemid 44563630)
132. Tengelsen L, Hudson R, Barnes S, Hahn C (2002) Coordinated response to reports of possible anthrax contamination, Idaho, 2001. Emerg Infect Dis 8:1093-1095 (Pubitemid 35257000)
133. Thavaselvam D, Vijayaraghavan R (2001) Biowarfare agents. J Pharm Bioallied Sci 2:179-188
134. Tims TB, Lim DV (2004) Rapid detection of Bacillus anthracis spores directly from powders with an evanescent wave fiber-optic biosensor. J Microbiol Methods 59:127-130 (Pubitemid 39119919)
135. Titball RW, Turnbull PC, Hutson RA (1991) The monitoring and detection of Bacillus anthracis in the environment. Soc Appl Bacteriol Symp Ser 70:S9-S18
136. Tourasse NJ, Helgason E, Økstad OA, Hegna IK, Kolstø AB (2006) The Bacillus cereus group: novel aspects of population structure and genome dynamics. J Appl Microbiol 101:579-593 (Pubitemid 44222288)
137. Tuerk C, Gold L (1990) Systematic evolution of ligands by exponential-enrichment: RNA ligands to tacteriophage-T4 DNA polymerase. Science 249:505-510
138. Turnbough CL (2003) Discovery of phage display peptide ligands for species-specific detection of Bacillus spores. J Microbiol Methods 53:263-271
139. Turnbull PCB (1999) Definitive identification of Bacillus anthracis-a review. J Appl Microbiol 87:237-240
140. Urwin R, Maiden MC (2003) Multi-locus sequence typing: a tool for global epidemiology. Trends Microbiol 11:479-487 (Pubitemid 37214875)
141. Valjevac S, Hilaire V, Lisanti O, Ramisse F, Hernandez E, Cavallo JD, Pourcel C, Vergnaud G (2005) Comparison of minisatellite polymorphisms in the Bacillus cereus complex: a simple assay for largescale screening and identification of strains most closely related to Bacillus anthracis. Appl Environ Microbiol 71:6613-6623 (Pubitemid 43194144)
142. Van der Auwera GA, Timmery S, Mahillon J (2008) Self-transfer and mobilisation capabilities of the pXO2-like plasmid pBT9727 from Bacillus thuringiensis subsp. konkukian 97-27. Plasmid 59:134-138
143. Velasco J, Romero C, López-Goñi I, Leiva J, Díaz R, Moriyón I (1998) Evaluation of the relatedness of Brucella spp. and Ochrobactrum anthropi and description of Ochrobactrum intermedium sp. nov. a new species with a closer relationship to Brucella spp. Int J Syst Bacteriol 48:759-768 (Pubitemid 28394655)
144. Volokhov D, Pomerantsev A, Kivovich V, Rasooly A, Chizhikov V (2004) Identification of Bacillus anthracis by multiprobe microarray hybridization. Diagn Microbiol Infect Dis 49:163-171 (Pubitemid 38891722)
145. Wang DB, Yang R, Zhang ZP, Bi LJ, You XY, Wei HP, ZhouY F, Yu Z, Zhang XE (2009) Detection of B. anthracis spores and vegetative cells with the same monoclonal antibodies. PloS one 4:e7810
146. Whittaker P (2009) Comparison of Yersinia pestis to other closely related Yersinia species using fatty acid profiles. Food Chem 116:629-632
147. Wielinga PR, de Heer L, de Groot A, Hamidjaja RA, Bruggeman G, Jordan K, van Rotterdam BJ (2011) Evaluation of DNA extraction methods for Bacillus anthracis spores spiked to food and feed matrices at biosafety level 3 conditions. Int J Food Microbiol 150:122-127
148. Williams DD, Benedek O, Turnbough CL Jr (2003) Species-specific peptide ligands for the detection of Bacillus anthracis spores. Appl Environ Microbiol 69:6288-6293 (Pubitemid 37280357)
149. Williams DD, Turnbough CL Jr (2004) Surface layer protein EA1 is not a component of Bacillus anthracis spores but is a persistent contaminant in spore preparations. J Bacteriol 186:566-569 (Pubitemid 38076446)
150. Yu H (1998) Comparative studies of magnetic particle-based solid phase fluorogenic and electrochemiluminescent immunoassay. J Immunol Methods 218:1-8
151. Zahavy E, Fisher M, Bromberg A, Olshevsky U (2003) Detection of frequency resonance energy transfer pair on double-labeled microsphere and Bacillus anthracis spores by flow cytometry. Appl Environ Microbiol 69:2330-2339 (Pubitemid 36443654)
152. Zasada AA, Gierczynski R, Raddadi N, Daffonchio DJ, Jagielski M (2006) Some Bacillus thuringiensis strains share rpoB nucleotide polymorphisms also present in Bacillus anthracis. J Clin Microbiol 44:1606-1607
153. Zilinskas RA (1997) Iraq's biological warfare program: the past as future? JAMA 278:418-424