Rapid and effective detection of anthrax spores in soil by PCR

Journal of Applied Microbiology

Volumn 95, Issue 4, 2003, Pages 728-733

  Cheun, H I ^a   Makino, Sou Ichi ^a   Watarai, M ^a   Erdenebaatar, J ^a,c   Kawamoto, K ^a   Uchida, I ^b  

^a OBIHIRO UNIVERSITY OF AGRICULTURE AND VETERINARY MEDICINE   (Japan)

^b NATIONAL INSTITUTE OF ANIMAL HEALTH   (Japan)

^c Institute of Veterinary Medicine   (Mongolia)

Author keywords

Bacillus anthracis; Detection; Nested PCR; Real time PCR; Soil

Indexed keywords

BACTERIA; BACTERIOLOGY; DISEASES; DNA; POLYMERASE CHAIN REACTION; SOIL POLLUTION;

ANTHRAX SPORE; B.ANTHRACIS; BACILLUS ANTHRACIS; DETECTION; DNA-TEMPLATE; ETHANOL-WATER; NESTED POLYMERASE CHAIN REACTION; REAL TIME POLYMERASE CHAIN REACTIONS; SIMPLE++; STERILE WATER;

SOILS;

BACTERIAL DNA;

BACTERIUM; DETECTION METHOD; POLYMERASE CHAIN REACTION; SOIL MICROORGANISM; SPORE;

ARTICLE; BACILLUS ANTHRACIS; BACTERIAL SPORE; BACTERIUM DETECTION; DNA TEMPLATE; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SOIL ANALYSIS;

ANTHRAX; BACILLUS ANTHRACIS; BACTERIA (MICROORGANISMS); POSIBACTERIA;

EID: 0141650391     PISSN: 13645072     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2672.2003.02038.x     Document Type: Article

References (14)

1. Asakura, H., Makino, S.-I., Shirahata, T., Tsukamoto, T., Kurazono, H., Ikeda, T. and Takeshi, K. (1998) Detection and long-term existence of Shiga toxin (Stx) producing Escherichia coli in sheep. Microbiology and Immunology 42, 683-688.
2. Cheun, H.I., Makino, S.-I., Watarai, M., Shirahata, T., Uchida, I. and Takeshi, K. (2001) A simple and sensitive detection system for Bacillus anthracis in meat and tissue. Journal of Applied Microbiology 91, 421-426.
3. Dragon, D.C. and Rennie, R.P. (2001) Evaluation of spore extraction and purification methods for selective recovery of viable Bacillus anthracis spores. Letters in Applied Microbiology 33, 100-105.
4. Etienne-Toumelin, I., Sirard, J., Duflot, E., Mock, M. and Fouet, A. (1995) Characterization of the Bacillus anthracis S-layer: cloning and sequencing of the structural gene. Journal of Bacteriology 177, 614-620.
5. Jernigan, J., Stephens, D., Ashford, D., Omenaca, C., Topiel, M., Galbraith, M., Tapper, M. and Fisk, T.L. (2001) Bioterrorism-related inhalational anthrax: the first 10 cases reported in the United States. Emerging Infectious Diseases 7, 933-944.
6. Knisely, P.F. (1966) Selective medium for Bacillus anthracis. Journal of Bacteriology 92, 784-786.
7. Makino, S.-I., Uchida, I., Terakado, N., Sasakawa, C. and Yochikawa, M. (1989) Molecular characterization and protein analysis of the cap region, which is essential for encapsulation in Bacillus anthracis. Journal of Bacteriology 171, 722-730.
8. Makino, S.-I., Cheun, H.I., Watarai, M., Uchida, I. and Takeshi, K. (2001) Detection of anthrax spores from the air by real-time PCR. Letters in Applied Microbiology 33, 237-240.
9. Mikessel, P., Ivins, B.E., Ristroph, J.D. and Dreier, T.M. (1983) Evidence for plasmid mediated toxin production in Bacillus anthracis. Injection and Immunity 39, 371-379.
10. Price, L.B., Hugh-Jones, M., Jackson, P.J. and Keim, P. (1999) Genetic diversity in the protective antigen gene of Bacillus anthracis. Journal of Bacteriology 181, 2358-2362.
11. Tebbe, C.C., Vahjen, W. (1993). Interference of humic acids and DNA extracted directly from soil in detection and transformation of recombinant DNA from bacteria and a yeast. Applied and Environmental Microbiology 59, 2657-2665.
12. Turnbull, P.C.B., Hutson, R.A., Ward, M.J., Jones, M.N., Quinn, P.C., Finnie, N.J., Duggleby, C.J., Kramer, J.M. et al. (1992) Bacillus anthracis but not always anthrax. Journal of Applied Microbiology 72, 21-28.
13. Uchida, I., Sekizaki, T., Hashimoto, K. and Terakado, N. (1985) Association of the encapsulation of Bacillus anthracis with a 60 megadalton plasmid. Journal of General Microbiology 131, 363-367.
14. Uchida, I., Hornung, J.M., Thorne, C.B., Klimpel, K.R. and Leppla, S.H. (1993) Cloning and characterization of a gene whose product is a trans-activator of anthrax toxin synthesis. Journal of Bacteriology 175, 5329-5338.