Primary metabolism in the new human cell line AGE1.HN at various substrate levels: Increased metabolic efficiency and α 1-antitrypsin production at reduced pyruvate load

Applied Microbiology and Biotechnology

Volumn 93, Issue 4, 2012, Pages 1637-1650

  Niklas, Jens ^a   Priesnitz, Christian ^a   Rose, Thomas ^b   Sandig, Volker ^b   Heinzle, Elmar ^a  

^a SAARLAND UNIVERSITY   (Germany)

^b PROBIOGEN AG   (Germany)

Author keywords

CHO; Human cell; Mammalian cell; Metabolic flux; Physiology; Recombinant protein

Indexed keywords

ANTITRYPSIN; CELL LINES; CHO; CHO CELL; DIFFERENT SUBSTRATES; ENERGY SPILLING; FEEDING PROFILE; FULL FACTORIAL DESIGN; GLUCOSE LEVEL; HUMAN CELL LINES; HUMAN CELLS; IN-VITRO; MAMMALIAN CELLS; METABOLIC FLUX; METABOLIC PHENOTYPE; PROCESS CONDITION; PRODUCTION CELLS; PYRUVATE METABOLISM; PYRUVATES; SUBSTRATE UPTAKE; TCA CYCLE; VARIOUS SUBSTRATES; VIABLE CELLS;

AMINO ACIDS; ANIMAL CELL CULTURE; BATCH CELL CULTURE; CELL PROLIFERATION; ENZYME ACTIVITY; FEEDING; METABOLISM; PHYSIOLOGY; RECOMBINANT PROTEINS;

SUBSTRATES;

ALPHA 1 ANTITRYPSIN; GLUCOSE; GLUTAMINE; PYRUVIC ACID;

ADAPTATION; CELL ORGANELLE; CONCENTRATION (COMPOSITION); DEVELOPMENTAL BIOLOGY; ENZYME ACTIVITY; GROWTH RATE; MAMMAL; METABOLISM; PHENOTYPE; PROTEIN; RECOMBINATION; SUBSTRATE;

ADAPTATION; ARTICLE; CELL GROWTH; CELL LINE; CELL LINE AGE1.HN; CELL METABOLISM; CELL PROLIFERATION; CELL VIABILITY; CHO CELL; CITRIC ACID CYCLE; CONCENTRATION RESPONSE; CULTURE MEDIUM; ENZYME SUBSTRATE; FED BATCH CULTURE; GLYCOLYSIS; HUMAN; HUMAN CELL; NERVE CELL;

ALPHA 1-ANTITRYPSIN; CELL LINE; CELL PROLIFERATION; CELL SURVIVAL; CULTURE MEDIA; GLUCOSE; GLUTAMINE; HUMANS; NEURONS; PYRUVIC ACID;

MAMMALIA;

EID: 84857924358     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3526-6     Document Type: Article

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