N-glycosylation and biological activity of recombinant human alpha1-antitrypsin expressed in a novel human neuronal cell line

Biotechnology and Bioengineering

Volumn 108, Issue 9, 2011, Pages 2118-2128

  Blanchard, Véronique ^a   Liu, Xi ^a   Eigel, Susann ^a   Kaup, Matthias ^a   Rieck, Silke ^a   Janciauskiene, Sabina ^b   Sandig, Volker ^c   Marx, Uwe ^c   Walden, Peter ^a   Tauber, Rudolf ^a   Berger, Markus ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^b HANNOVER MEDICAL SCHOOL   (Germany)

^c PROBIOGEN AG   (Germany)

Author keywords

1 antitrypsin; Anti inflammatory activity; Human neuronal cell line; Lewis X; N glycosylation

Indexed keywords

ANTI-INFLAMMATORY ACTIVITY; ANTITRYPSIN; LEWIS X; N-GLYCOSYLATION; NEURONAL CELL;

AMINO ACIDS; BODY FLUIDS; CELL CULTURE; ESTERIFICATION; GLYCOPROTEINS; GLYCOSYLATION; LAKES; MASS SPECTROMETRY; PURIFICATION; TISSUE;

ENZYME INHIBITION;

ALPHA 1 ANTITRYPSIN; ELASTASE;

ANTIINFLAMMATORY ACTIVITY; ARTICLE; BIOLOGICAL ACTIVITY; CONTROLLED STUDY; ENZYME METABOLISM; FUCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NERVE CELL; PROTEIN ANALYSIS; PROTEIN GLYCOSYLATION; PROTEIN STRUCTURE; SIALYLATION;

ALPHA 1-ANTITRYPSIN; ANTI-INFLAMMATORY AGENTS; BIOTECHNOLOGY; CELL LINE; CELLS, CULTURED; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLYCOSYLATION; HUMANS; LEUKOCYTE ELASTASE; MONOCYTES; NEURONS; NEUTROPHILS; RECOMBINANT PROTEINS; SIALIC ACIDS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TUMOR NECROSIS FACTOR-ALPHA;

EID: 79960348248     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.23158     Document Type: Article

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