Comparison of metabolic flux distributions for MDCK cell growth in glutamine- and pyruvate-containing media

Biotechnology Progress

Volumn 24, Issue 2, 2008, Pages 311-320

  Sidorenko, Yury ^a   Wahl, Aljoscha ^a   Dauner, Michael ^a,b   Genzel, Yvonne ^a   Reichl, Udo ^a,c  

^a MAX PLANCK INSTITUTE FOR DYNAMICS OF COMPLEX TECHNICAL SYSTEMS   (Germany)

^b DUPONT COMPANY   (United States)

^c OTTO VON GUERICKE UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINETRIPHOSPHATE; AMINO ACIDS; AMMONIA; ANIMAL CELL CULTURE; BIOSYNTHESIS; DEGRADATION; GLUCOSE; METABOLISM;

CELL LINES; EXCRETED AMMONIA; HYBRIDOMA CELLS; MAMMALIAN CELLS;

CELL GROWTH;

ADENOSINE TRIPHOSPHATE; AMINO ACID; GLUTAMINE; PYRUVIC ACID;

ACTIVE TRANSPORT; ANIMAL; ARTICLE; CELL LINE; CITRIC ACID CYCLE; COMPARATIVE STUDY; CULTURE MEDIUM; CYTOLOGY; CYTOPLASM; DOG; GLYCOLYSIS; GROWTH, DEVELOPMENT AND AGING; KIDNEY; METABOLISM; MITOCHONDRION; OXIDATION REDUCTION REACTION; OXYGEN CONSUMPTION; PENTOSE PHOSPHATE CYCLE; PHYSIOLOGY; STATISTICAL MODEL;

ADENOSINE TRIPHOSPHATE; AMINO ACIDS; ANIMALS; BIOLOGICAL TRANSPORT, ACTIVE; CELL LINE; CITRIC ACID CYCLE; CULTURE MEDIA; CYTOPLASM; DOGS; GLUTAMINE; GLYCOLYSIS; KIDNEY; MITOCHONDRIA; MODELS, STATISTICAL; OXIDATION-REDUCTION; OXYGEN CONSUMPTION; PENTOSE PHOSPHATE PATHWAY; PYRUVIC ACID;

MAMMALIA;

EID: 42049113587     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp0702673     Document Type: Article

References (55)

1. Schneider, M.; Marison, I. W.; von Stockar, U. The importance of ammonia in mammalian cell culture. J. Biotechnol. 1996, 46 (3), 161-185.
2. Glacken, M. W.; Fleischaker, R. J.; Sinskey, A. J. Reproduction of waste product excretion via nutrient control: possible strategies for maximizing product and cell yields on serum in cultures of mammalian cells. Biotechnol. Bioeng. 1986, 28, 1376-1389.
3. Donelly, M.; Scheffler, I. E. Energy metabolism in respiration-deficient and wild type Chinese hamster fibroblasts in culture. J. Cell Physiol. 1976, 89 (1), 39-51.
4. Reitzer, L. J.; Wice, B. M.; Kennell, D. Evidence that glutamine, not sugar, is the major energy source for cultured HeLa cells. J. Biol. Chem. 1979, 254, 2669-2676.
5. Moreadith, R. W.; Lehninger, A. L. The pathways of glutamate and glutamine oxidation by tumor cell mitochondria. Role of mitochondrial NAD(P)+-dependent malic enzyme. J. Biol. Chem. 1984, 259 (10), 6215-6221.
6. Sharfstein, S. T.; Tucker, S. N.; Mancuso, A.; Blanch, H. W.; Clark, D. S. Quantitative in vivo nuclear magnetic resonance studies of hybridoma metabolism. Biotechnol. Bioeng. 1994, 43 (11), 1059-1074.
7. Dalili, M.; Sayles, G. D.; Ollis, D. F. Glutamine-limited batch hybridoma growth and antibody production: experiment and model. Biotechnol. Bioeng. 1990, 36, 74-82.
8. Omasa, T.; Ishimoto, M.; Higashiyama, K.; Shioya, S.; Suga, K. The enhancement of specific antibody production rate in glucose-and glutamine-controlled fed-batch culture. Cytotechnology 1992, 8 (1), 75-84.
9. Miller, W. M.; Wilke, C. R.; Blanch, H. W. Transient responces of hybridoma cells to lactate and ammonia pulse and step changes in continuous culture. Bioprocess Eng. 1988, 3, 113-122.
10. Hassell, T.; Gleave, S.; Butler, M. Growth inhibition in animal cell culture. The effect of lactate and ammonia. Appl. Biochem. Biotechnol. 1991, 30 (1), 29-41.
11. Hassell, T.; Butler, M. Adaptation to non-ammoniagenic medium and selective substrate feeding lead to enhanced yields in animal cell cultures. J. Cell Sci. 1990, 96, 501-508.
12. McDermott, R. H.; Butler, M. Uptake of glutamate, not glutamine synthetase, regulates adaptation of mammalian cells to glutamine-free medium. J. Cell Sci. 1993, 104, 51-58.
13. Genzel, Y.; Ritter, J. B.; Konig, S.; Alt, R.; Reichl, U. Substitution of glutamine by pyruvate to reduce ammonia formation and growth inhibition of mammalian cells. Biotechnol. Prog. 2005, 21 (1), 58-69.
14. Stephanopoulos, G.; Aristidou, A. A.; Nielsen, J. Metabolic Engineering; Academic Press: San Diego, 1998.
15. Van Der Heijden, R. T. J. M.; Heijnen, J. J.; Hellinga, C.; Romein, B.; Luyben, K. C. A. M. Linear constraint relations in biochemical reaction systems: I. Classification of the calculability and the balanceability of conversion rates. Biotechnol. Bioeng. 1994, 43 (1), 3-10.
16. Klamt, S.; Schuster, S.; Gilles, E. D. Calculability analysis in underdetermined metabolic networks illustrated by a model of the central metabolism in purple nonsulfur bacteria. Biotechnol. Bioeng. 2002, 77 (7), 734-751.
17. Wiechert, W. 13C metabolic flux analysis. Metab. Eng. 2001, 3 (3), 195-206.
18. Gombert, A. K.; Nielsen, J. Mathematical modelling of metabolism. Curr. Opin. Biotechnol. 2000, 11 (2), 180-186.
19. Hua, Q.; Yang, C.; Shimizu, K. Metabolic flux analysis for efficient pyruvate fermentation using vitamin-auxotrophic yeast of Torulopsis glabrata. J. Biosci. Bioeng. 1999, 87 (2), 206-213.
20. Granstrom, T. B.; Aristidou, A.; Jokela, J.; Leisola, M. Growth characteristics and metabolic flux analysis of Candida milleri. Biotechnol. Bioeng. 2002, 70 (2), 197-207.
21. Herwig, C.; von Stockar, U. A small metabolic flux model to identify transient metabolic regulations in Saccharomyces cerevisiae. Bioprocess Biosys. Eng. 2002, 24 (6), 395-403.
22. Rizzi, M.; Baltes, M.; Theobald, U.; Reuss, M. In vivo analysis of metabolic dynamics in Saccharomyces cerevisiae: II. Mathematical model. Biotechnol. Bioeng. 1997, 55 (4), 592-608.
23. Forster, J.; Famili, I.; Fu, P.; Palsson, B. O.; Nielsen, J. Genome-scale reconstruction of the Saccharomyces cerevisiae metabolic network. Genet. Res. 2003, 13 (2), 244-253.
24. Dauner, M.; Sauer, U. Stoichiometric growth model for riboflavin-producing Bacillus subtilis. Biotechnol. Bioeng. 2001, 76 (2), 132-143.
25. Zhao, J.; Shimizu, K. Metabolic flux analysis of Escherichia coli K12 grown on 13C-labeled acetate and glucose using GC-MS and powerful flux calculation method. J. Biotechnol. 2002, 101 (2), 101-117.
26. Poughon, L.; Dussap, C. G.; Gros, J. B. Energy model and metabolic flux analysis for autotrophic nitrifiers. Biotechnol. Bioeng. 2001, 72 (4), 416-433.
27. Calik, P.; Calik, G.; Takac, S.; Ozdamar, T. H. Metabolic flux analysis for serine alkaline protease fermentation by Bacillus licheniformis in a defined medium: Effects of the oxygen transfer rate. Biotechnol. Bioeng. 1999, 64 (2), 151-167.
28. Savinell, J.; Pallson, B. O. Network analysis of intermediary metabolism using linear optimisation. I. Development of mathematical formalism. J. Theor. Biol. 1992, 154 (4), 421-454.
29. Bonarius, H. P.; Houtman, J. H.; de Gooijer, C. D.; Tramper, J.; Schmid, G. Activity of glutamate dehydrogenase is increased in ammonia-stressed hybridoma cells. Biotechnol Bioeng. 1998, 57 (4), 447-453.
30. Zupke, C.; Sinskey, A. J.; Stephanopoulos, G. Intracellular flux analysis applied to the effect of dissolved oxygen on hybridomas. Appl. Microbiol. Biotechnol. 1995, 44 (1-2), 27-36.
31. Follstad, B. D.; Balcarcel, R. R.; Stephanopoulos, G.; Wang, D. I. Metabolic flux analysis of hybridoma continuous culture steady state multiplicity. Biotechnol. Bioeng. 1999, 63 (6), 675-683.
32. Sheikh, K.; Forster, J.; Nielsen, L. K. Modeling hybridoma cell metabolism using a generic genome-scale metabolic model of Mus musculus. Biotechnol. Prog. 2005, 21 (1), 112-121.
33. Nyberg, G. B.; Balcarcel, R. R.; Follstad, B. D.; Stephanopoulos, G.; Wang, D. I. Metabolism of peptide amino acids by Chinese hamster ovary cells grown in a complex medium. Biotechnol. Bioeng. 1999, 62 (3), 324-335.
34. Cruz, H. J.; Moreira, J. L.; Carrondo, M. J. T. Metabolic shifts by nutrient manipulation in continuous cultures of BHK cells. Biotechnol. Bioeng. 1999, 66 (2), 104-113.
35. Chan, C.; Berthiaume, F.; Lee, K.; Yarmush, M. L. Metabolic flux analysis of cultured hepatocytes exposed to plasma. Biotechnol. Bioeng. 2003, 81 (1), 33-49.
36. Genzel, Y.; Behrendt, I.; Konig, S.; Sann, H.; Reichl, U. Metabolism of MDCK cells during cell growth and influenza virus production in large-scale microcarrier culture. Vaccine 2004, 22 (17-18), 2202-2208.
37. Wahl, A.; Sidorenko, Y.; Dauner, M.; Genzel, Y.; Reichl, U. Metabolic model for a MDCK cell line. Minimum Substrate Flux Distribution and Phases of Stationarity. Submitted for publication.
38. Genzel, Y.; Fischer, M.; Reichl, U. Serum-free influenza virus production avoiding washing steps and medium exchange in large-scale microcarrier culture. Vaccine 2006, 24 (16), 3261-3272.
39. Genzel, Y.; Konig, S.; Reichl, U. Amino acid analysis in mammalian cell culture media containing serum and high glucose concentrations by anion exchange chromatography and integrated pulsed amperometric detection. Anal. Biochem. 2004, 335 (1), 119-125.
40. Salway, J. G. Metabolism at a Glance; Blackwell Publishing: Cambridge, 1999.
41. Nelson, D. L.; Cox, M. M.; Lehninger, A. L. Lehninger Principles of Biochemistry, 3rd ed.; Worth Publishers, Inc.: New York, 2000.
42. KEGG; Kyoto Encyclopedia of Genes and Genomes; http://www.genome.ad.jp/kegg/.
43. BRENDA; Enzyme Database; http://www.brenda.uni-koeln.de/.
44. Bonarius, H. P. J.; Hatzimanikatis, V.; Meesters, K. P. H.; de Gooijer, C. D.; Schmid, G.; Tramper, J. Metabolic flux analysis of hybridoma cells in different culture media using mass balances. Biotechnol. Bioeng. 1996, 50 (3), 299-318.
45. Popov, V. N.; Moskalev, E. A.; Shevchenko, M. U.; Eprintsev, A. T. Comparative analysis of glyoxylate cycle key enzyme isocitrate lyase from organisms of different systematic groups. J. Evol. Biochem. Physiol. 2005, 41 (6), 631-639.
46. Haggstrom, L. Cell metabolism, animal. In Encyclopaedia of Cell Technology; Stier, R., Ed.; Wiley & Sons: New York, 2000; pp 392-411.
47. Neermann, J.; Wagner, R. Comparative analysis of glucose and glutamine metabolism in transformed mammalian cell lines, insect and primary liver cells. J. Cell Physiol. 1996, 166 (1), 152-169.
48. Vriezen, N.; van Dijken, J. P. Fluxes and enzyme activities in central metabolism of myeloma cells grown in chemostat culture. Biotechnol. Bioeng. 1998, 59 (1), 28-39.
49. Lazo, P. A. Amino acids and glucose utilization by different metabolic pathways in ascites-tumour cells. Eur. J. Biochem. 1981, 117 (1), 19-25.
50. Altamirano, C.; Illanes, A.; Becerra, S.; Cairy, J. J.; Godia, F. Considerations on the lactate consumption by CHO cells in the presence of galactose. J. Biotechnol. 2006, 125 (4), 547-556.
51. Provost, A.; Bastin, G. Dynamic metabolic modeling under the balanced growth conditions. J. Process Control 2004, 14, 717-728.
52. Zupke, C.; Stephanopoulos, G. Intracellular flux analysis in hybridomas using mass balances and in vitro 13C NMR. Biotechnol. Bioeng. 1995, 45, 292-303.
53. Dunlop, J.; McIlvain, H. B.; Lou, Z. W.; Franco, R. The pharmacological profile of L-glutamate transport in human NT2 neurones is consistent with excitatory amino acid transporter 2. Eur. J. Pharmacol. 1998, 360 (2-3), 249-256.
54. McGivan, J. D.; Pastor-Anglada, M. Regulatory and molecular aspects of mammalian amino acid transport. Biochem. J. 1994, 299, 321-334.
55. Bussolati, O.; Uggeri, J.; Belletti, S.; Dall'Asta, V.; Gazzola, G. C. The stimulation of Na, K, Cl cotransport and of system A for neutral amino acid transport is a mechanism for cell volume increase during the cell cycle. FASEB J. 1996, 10 (8), 920-926.