Genealogy profiling through strain improvement by using metabolic network analysis: Metabolic flux genealogy of several generations of lysine-producing Corynebacteria

Applied and Environmental Microbiology

Volumn 68, Issue 12, 2002, Pages 5843-5859

  Wittmann, Christoph ^a   Heinzle, Elmar ^a  

^a SAARLAND UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOXYLATION; CARBOXYLIC ACIDS; GAS CHROMATOGRAPHY; LASER APPLICATIONS; MASS SPECTROMETRY; METABOLISM; MONTE CARLO METHODS; STATISTICAL METHODS; TRACE ANALYSIS;

GENEALOGY PROFILING;

MICROBIOLOGY;

CARBON 13; GLUCOSE; LYSINE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

GENEALOGY;

ACCURACY; ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BIOTECHNOLOGICAL PROCEDURES; CARBOXYLATION; CONTROLLED STUDY; CORYNEBACTERIUM GLUTAMICUM; DECARBOXYLATION; GENEALOGY; GLUCOSE TRANSPORT; MONTE CARLO METHOD; NONHUMAN; PENTOSE PHOSPHATE CYCLE; REGULATORY MECHANISM; STRAIN DIFFERENCE;

CITRIC ACID CYCLE; CORYNEBACTERIUM; GENEALOGY AND HERALDRY; GLUCOSE-6-PHOSPHATE; GLYCOLYSIS; GLYOXYLATES; LYSINE; MASS SPECTROMETRY; NADP; PENTOSE PHOSPHATE PATHWAY; PYRUVIC ACID;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); CORYNEBACTERIA; CORYNEBACTERIUM; CORYNEBACTERIUM GLUTAMICUM ATCC 13032; UNCULTURED ACTINOMYCETE;

EID: 0036956758     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.68.12.5843-5859.2002     Document Type: Article

References (34)

1. Dauner, M., J. E. Bailey, and U. Sauer. 2001. Metabolic flux analysis with a comprehensive isotopomer model in Bacillus subtilis. Biotechnol. Bioeng. 76:144-156.
2. de Graaf, A. A., L. Eggeling, and H. Sahm. 2001. Metabolic engineering for L-lysine production by Corynebacterium glutamicum. Adv. Biochem. Eng. Biotechnol. 73:9-29.
3. Eikmanns, B. J., D. Rittmann, and H. Sahm. 1995. Cloning, sequence analysis, expression, and inactivation of the Corynebacterium glutamicum icd gene encoding isocitrate dehydrogenase and biochemical characterization of the enzyme. J. Bacteriol. 177:774-782.
4. Kiss, R. D., and G. Stephanopoulos. 1992. Metabolic characterization of a L-lysine-producing strain by continuous culture. Biotechnol. Bioeng. 39:565-574.
5. Kitson, F. G., B. Larsen, and C. N. McEven. 1996. Gas chromatography and mass spectrometry: a practical guide. Academic Press, San Diego, Calif.
6. Marx, A., A. A. de Graaf, W. Wiechert, L. Eggeling, and H. Sahm. 1996. Determination of the fluxes in the central metabolism of Corynebacterium glutamicum by nuclear magnetic resonance spectroscopy combined with metabolite balancing. Biotechnol. Bioeng. 49:111-129.
7. Marx, A., B. Eikmanns, H. Sahm, A. A. de Graaf, and L. Eggeling. 1999. Response of the central metabolism in Corynebacterium glutamicum to the use of an NADH-dependent glutamate dehydrogenase. Metabol. Eng. 1:35-48.
8. Matsushita, K., A. Otofuji, M. Iwahashi, H. Toyama, and O. Adachi. 2001. NADH dehydrogenase of Corynebacterium glutamicum. Purification of an NADH dehydrogenase II homolog able to oxidize NADPH. FEMS Microbiol. Lett. 204:271-276.
9. Michal, G. 1999. Biochemical pathways. Spektrum Akademischer Verlag, Heidelberg, Germany.
10. Möllney, M., W. Wiechert, D. Kownatzki, and A. A. de Graaf. 1999. Bidirectional reaction steps in metabolic networks, part IV: optimal design of isotopomer labeling experiments. Biotechnol. Bioeng. 66:86-103.
11. Moritz, B., K. Striegel, A. A. de Graaf, and H. Sahm. 2000. Kinetic properties of the glucose-6-phosphate and 6-phosphogluconate dehydrogenases from Corynebacterium glutamicum and their application for predicting pentose phosphate pathway flux in vivo. Eur. J. Biochem. 267:3442-3452.
12. Nakayama, K., and K. Araki. January 1973. U.S. patent 3,708,395.
13. Ohnishi, J., S. Mitsuhashi, M. Hayashi, S. Ando, H. Yokoi, K. Ochiai, and M. A. Ikeda. 2002. A novel methodology employing Corynebacterium glutamicum genome information to generate a new L-lysine-producing mutant. Appl. Microbiol. Biotechnol. 58:217-223.
14. Petersen, S., A. A. De Graaf, L. Eggeling, M. Möllney, W. Wiechert, and H. Sahm. 2000. In vivo quantification of parallel and bidirectional fluxes in the anaplerosis of Corynebacterium glutamicum. J. Biol. Chem. 275:35932-35941.
15. Rosman, K. J. R., and P. D. P. Taylor. 1998. Isotopic compositions of the elements 1997. Pure Appl. Chem. 70:217-235.
16. Rowlands, R. T. 1984. Industrial strain improvement: mutagenesis and random screening procedures. Enzyme Microb. Technol. 6:3-10.
17. Sahm, H., L. Eggeling, and A. A. de Graaf. 2000. Pathway analysis and metabolic engineering in Corynebacterium glutamicum. Biol. Chem. 381:899-910.
18. Schmidt, K., M. Carlsen, J. Nielsen, and J. Villadsen. 1997. Modeling isotopomer distributions in biochemical networks using isotopomer mapping matrices. Biotechnol. Bioeng. 55:831-840.
19. 13C coupling constraints on the isotopomer distribution in labeled biomass components. Metabol. Eng. 1:166-179.
20. Shiio, I., H. Momose, and A. Oyama. 1969. Genetic and biochemical studies on bacterial formation of glutamate. I. Relationship between isocitrate lyase, acetate kinase and phosphate acetyltransferase levels and glutamate production in Brevibacterium flavum. J. Gen. Appl. Microbiol. 15:27-40.
21. 1H-NMR spectroscopy. Eur. J. Biochem. 213:1325-1331.
22. 13C NMR studies of the fluxes in the central metabolism of Corynebacterium glutamicum during growth and overproduction of amino acids in batch cultures. Appl. Microbiol. Biotechnol. 44:489-495.
23. Stephanopoulos, G., A. A. Aristidou, and J. Nielsen. 1998. Metabolic engineering. Academic Press, San Diego, Calif.
24. Sugimoto, S. I., and I. Shiio. 1987. Regulation of glucose 6-phosphate dehydrogenase in Brevibacterium flavum. Agric. Biol. Chem. 51:101-108.
25. Sugimoto, S. I., and I. Shiio. 1987. Regulation of 6-phosphogluconate dehydrogenase in Brevibacterium flavum. Agric. Biol. Chem. 51:1257-1263.
26. Vallino, J. J., and G. Stephanopoulos. 1993. Metabolic flux distributions in Corynebacterium glutamicum during growth and lysine overproduction. Biotechnol. Bioeng. 41:633-646.
27. Van Winden, W. A., C. Wittmann, E. Heinzle, and J. J. Heijnen. 2002. Correcting mass isotopomer distributions for naturally occurring isotopes. Biotechnol. Bioeng. 80:477-479.
28. Wendisch, V. F., M. Spies, D. J. Reinscheid, S. Schnicke, H. Sahm, and B. J. Eiksmanns. 1997. Regulation of acetate metabolism in Corynebacterium glutamicum: transcriptional control of the isocitrate lyase and malate synthase genes. Arch. Microbiol. 168:262-269.
29. Wendisch, V. F., A. A. de Graaf, H. Sahm, and B. J. Eikmanns. 2000. Quantitative determination of metabolic fluxes during coutilization of two carbon sources: comparative analyses with Corynebacterium glutamicum during growth on acetate and/or glucose. J. Bacteriol. 182:3088-3096.
30. Wiechert, W., C. Siefke, A. A. de Graaf, and A. Marx. 1997. Bidirectional reaction steps in metabolic networks: II. Flux estimation and statistical analysis. Biotechnol. Bioeng. 55:118-135.
31. Wittmann, C., and E. Heinzle. 1999. Mass spectrometry for metabolic flux analysis. Biotechnol. Bioeng. 62:739-750.
32. Wittmann, C., and E. Heinzle. 2001. Novel approach for metabolic flux analysis - application of MALDI-TOF MS to lysine-producing Corynebacterium glutamicum. Eur. J. Biochem. 268:2441-2455.
33. Wittmann, C., and E. Heinzle. 2001. Modeling and experimental design for metabolic flux analysis of lysine-producing Corynebacteria by mass spectrometry. Metabol. Eng. 3:173-191.
34. Wittmann, C. 2002. Metabolic flux analysis using mass spectrometry. Adv. Biochem. Eng. Biotechnol. 74:39-64.