Selective permeabilization for the high-throughput measurement of compartmented enzyme activities in mammalian cells

Analytical Biochemistry

Volumn 416, Issue 2, 2011, Pages 218-227

  Niklas, Jens ^a   Melnyk, Armin ^a   Yuan, Yongbo ^a   Heinzle, Elmar ^a  

^a SAARLAND UNIVERSITY   (Germany)

Author keywords

CHO; Compartmentation; Kinetics; Mammalian cell; Metabolic flux; Metabolism

Indexed keywords

CELL CULTURE; CELL MEMBRANES; ENZYME KINETICS; MAMMALS; METABOLISM; MITOCHONDRIA;

ACTIVITY MEASUREMENTS; COMPARTMENTATION; HIGH-THROUGHPUT ANALYSIS; HIGH-THROUGHPUT MEASUREMENTS; MAMMALIAN CELLS; METABOLIC FLUX; MITOCHONDRIAL MEMBRANES; SUBCELLULAR LOCALIZATIONS;

ENZYME ACTIVITY;

CELL ENZYME; DIGITONIN; ENZYME; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; OCTOXINOL;

ARTICLE; CELL COMPARTMENTALIZATION; CELL MEMBRANE; CELL PERMEABILIZATION; CELL STRAIN HEPG2; CELL SUSPENSION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME LOCALIZATION; ENZYME RELEASE; MEASUREMENT; MITOCHONDRIAL MEMBRANE; PRIORITY JOURNAL; ULTRASOUND; ANIMAL; CELL MEMBRANE PERMEABILITY; CHEMISTRY; CHO CELL; CRICETULUS; HAMSTER; HIGH THROUGHPUT SCREENING; HUMAN; KINETICS; METABOLISM; METHODOLOGY; MITOCHONDRION;

MAMMALIA;

ANIMALS; CELL MEMBRANE PERMEABILITY; CHO CELLS; CRICETINAE; CRICETULUS; DIGITONIN; ENZYMES; GLUCOSEPHOSPHATE DEHYDROGENASE; HEP G2 CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; KINETICS; MITOCHONDRIA; OCTOXYNOL;

EID: 80052643002     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.05.039     Document Type: Article

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