Anti-apoptotic genes aven and E1B-19K enhance performance of BHK cells engineered to express recombinant factor VIII in batch and low perfusion cell culture

Biotechnology and Bioengineering

Volumn 98, Issue 4, 2007, Pages 825-841

  Nivitchanyong, Toey ^a   Martinez, Amanda ^b   Ishaque, Adiba ^b   Murphy, John E ^b   Konstantinov, Konstantin ^b   Betenbaugh, Michael J ^a   Thrift, John ^b  

^a JOHNS HOPKINS UNIVERSITY   (United States)

^b Analytics and Formulation Department Process Sciences Bayer Biotechnology   (United States)

Author keywords

Anti apoptosis engineering; Baby hamster kidney; E1B 19K; Factor VIII; Mammalian cell culture; Metabolic and cell engineering; Perfusion

Indexed keywords

ANTI-APOPTOSIS ENGINEERING; BABY HAMSTER KIDNEY; MAMMALIAN CELL CULTURE; METABOLIC AND CELL ENGINEERING;

AMMONIA; BIOLOGICAL ORGANS; BIOREACTORS; GENES; GLUCOSE;

CELL CULTURE;

AMMONIA; APOPTOSIS REGULATORY PROTEIN; CASPASE 3; E1B 19K PROTEIN; E1B PROTEIN; GLUCOSE; GLUTAMINE; LACTIC ACID; LIPOCORTIN 5; PROTEIN AVEN; RECOMBINANT BLOOD CLOTTING FACTOR 8; THAPSIGARGIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; APOPTOSIS; ARTICLE; BIOREACTOR; CELL CULTURE; CELL GROWTH; CELL SIZE; CELL SURVIVAL; CELL VIABILITY; CONTROLLED STUDY; ENZYME ACTIVATION; NONHUMAN; PROTEIN EXPRESSION;

ADAPTOR PROTEINS, SIGNAL TRANSDUCING; ANIMALS; APOPTOSIS; APOPTOSIS REGULATORY PROTEINS; CELL CULTURE TECHNIQUES; CELL LINE; CRICETINAE; FACTOR VIII; GENETIC ENHANCEMENT; KIDNEY; MEMBRANE PROTEINS; PERFUSION; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; VIRAL PROTEINS;

CRICETINAE; MAMMALIA;

EID: 35649024330     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21479     Document Type: Article

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