Second-contact shell mutation diminishes streptavidin-biotin binding affinity through transmitted effects on equilibrium dynamics

Biochemistry

Volumn 51, Issue 2, 2012, Pages 597-607

  Baugh, Loren ^a   Le Trong, Isolde ^b   Cerutti, David S ^c   Mehta, Nital ^c   Gülich, Susanne ^a   Stayton, Patrick S ^a   Stenkamp, Ronald E ^b   Lybrand, Terry P ^c  

^a University of Washington   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

^c VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ATOMIC FLUCTUATIONS; BINDING AFFINITIES; BINDING ENTHALPIES; BOUND STRUCTURE; EQUILIBRIUM DYNAMICS; EQUILIBRIUM STRUCTURES; HIGH AFFINITY; HYDROXYL GROUPS; LIGAND BINDING; MOLECULAR DYNAMICS SIMULATIONS; PEPTIDE BACKBONES; POINT MUTATIONS; PROTEIN DYNAMICS; SMALL GAIN; STREPTAVIDIN; STREPTAVIDIN-BIOTIN COMPLEX; WATER MOLECULE; WILD TYPES;

AMINO ACIDS; ATOMS; BINDING ENERGY; DISSOCIATION; DYNAMICS; HYDROGEN BONDS; LIGANDS; MOLECULAR DYNAMICS; PROTEINS;

COENZYMES;

BIOTIN; HYDROXYL GROUP; STREPTAVIDIN; TRYPTOPHAN;

ARTICLE; BINDING AFFINITY; CRYSTALLIZATION; DISSOCIATION; ENTHALPY; ENTROPY; EQUILIBRIUM CONSTANT; HYDROGEN BOND; LIGAND BINDING; MOLECULAR DYNAMICS; POINT MUTATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; RESIDUE ANALYSIS; THERMODYNAMICS; X RAY CRYSTALLOGRAPHY;

BINDING SITES; BIOTIN; CRYSTALLOGRAPHY, X-RAY; KINETICS; MOLECULAR DYNAMICS SIMULATION; MUTANT PROTEINS; POINT MUTATION; PROTEIN BINDING; PROTEIN CONFORMATION; STREPTAVIDIN; THERMODYNAMICS;

EID: 84855919905     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi201221j     Document Type: Article

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