Assessment of hypoxia inducible factor levels in cancer cell lines upon hypoxic induction using a novel reporter construct

PLoS ONE

Volumn 6, Issue 11, 2011, Pages

  Zhou, Wenyu ^a   Dosey, Timothy L ^a,b   Biechele, Travis ^a,b   Moon, Randall T ^a,b   Horwitz, Marshall S ^a   Ruohola Baker, Hannele ^a,b  

^a UNIVERSITY OF WASHINGTON   (United States)

^b UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HEAT SHOCK PROTEIN 90; HYPOXIA INDUCIBLE FACTOR; VON HIPPEL LINDAU PROTEIN; YELLOW FLUORESCENT PROTEIN; 17-(ALLYLAMINO)-17-DEMETHOXYGELDANAMYCIN; BENZOQUINONE DERIVATIVE; HYPOXIA INDUCIBLE FACTOR 1ALPHA; MACROCYCLIC LACTAM; MESSENGER RNA; OXYGEN; TANESPIMYCIN;

ARTICLE; BINDING SITE; CANCER CELL CULTURE; CELL HYPOXIA; CELL SPECIFICITY; CONTROLLED STUDY; FEMALE; GENE CONSTRUCT; GENE SEQUENCE; HUMAN; HUMAN CELL; PROTEIN DEGRADATION; PROTEIN FUNCTION; REPORTER CONSTRUCT; SIGNAL TRANSDUCTION; TANDEM REPEAT; UTERINE CERVIX CANCER; DRUG EFFECT; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETICS; METABOLISM; NUCLEOTIDE REPEAT; PROTEIN BINDING; REPORTER GENE; TUMOR CELL LINE;

BENZOQUINONES; CELL HYPOXIA; CELL LINE, TUMOR; GENE EXPRESSION REGULATION, NEOPLASTIC; GENES, REPORTER; HSP90 HEAT-SHOCK PROTEINS; HUMANS; HYPOXIA-INDUCIBLE FACTOR 1, ALPHA SUBUNIT; LACTAMS, MACROCYCLIC; OXYGEN; PROTEIN BINDING; REPETITIVE SEQUENCES, NUCLEIC ACID; RNA, MESSENGER; TRANSCRIPTION, GENETIC;

EID: 81755184035     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0027460     Document Type: Article

References (49)

1. Wang GL, Jiang BH, Rue EA, Semenza GL, (1995) Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension. Proc Natl Acad Sci U S A 92: 5510-5514.
2. Jain S, Maltepe E, Lu MM, Simon C, Bradfield CA, (1998) Expression of ARNT, ARNT2, HIF1 alpha, HIF2 alpha and Ah receptor mRNAs in the developing mouse. Mech Dev 73: 117-123.
3. Maxwell PH, Wiesener MS, Chang GW, Clifford SC, Vaux EC, et al. (1999) The tumour suppressor protein VHL targets hypoxia-inducible factors for oxygen-dependent proteolysis. Nature 399: 271-275.
4. Ohh M, Park CW, Ivan M, Hoffman MA, Kim TY, et al. (2000) Ubiquitination of hypoxia-inducible factor requires direct binding to the beta-domain of the von Hippel-Lindau protein. Nat Cell Biol 2: 423-427.
5. Pouyssegur J, Dayan F, Mazure NM, (2006) Hypoxia signalling in cancer and approaches to enforce tumour regression. Nature 441: 437-443.
6. Pollard PJ, Briere JJ, Alam NA, Barwell J, Barclay E, et al. (2005) Accumulation of Krebs cycle intermediates and over-expression of HIF1alpha in tumours which result from germline FH and SDH mutations. Hum Mol Genet 14: 2231-2239.
7. Bardos JI, Ashcroft M, (2005) Negative and positive regulation of HIF-1: a complex network. Biochim Biophys Acta 1755: 107-120.
8. Xenaki G, Ontikatze T, Rajendran R, Stratford IJ, Dive C, et al. (2008) PCAF is an HIF-1alpha cofactor that regulates p53 transcriptional activity in hypoxia. Oncogene 27: 5785-5796.
9. Isaacs JS, Jung YJ, Mimnaugh EG, Martinez A, Cuttitta F, et al. (2002) Hsp90 regulates a von Hippel Lindau-independent hypoxia-inducible factor-1 alpha-degradative pathway. J Biol Chem 277: 29936-29944.
10. Katschinski DM, Le L, Schindler SG, Thomas T, Voss AK, et al. (2004) Interaction of the PAS B domain with HSP90 accelerates hypoxia-inducible factor-1alpha stabilization. Cell Physiol Biochem 14: 351-360.
11. Liu YV, Baek JH, Zhang H, Diez R, Cole RN, et al. (2007) RACK1 Competes with HSP90 for Binding to HIF-1α and Is Required for O2-Independent and HSP90 Inhibitor-Induced Degradation of HIF-1α. Molecular Cell 25: 207-217.
12. Shweiki D, Itin A, Soffer D, Keshet E, (1992) Vascular endothelial growth factor induced by hypoxia may mediate hypoxia-initiated angiogenesis. Nature 359: 843-845.
13. Plate KH, Breier G, Weich HA, Risau W, (1992) Vascular endothelial growth factor is a potential tumour angiogenesis factor in human gliomas in vivo. Nature 359: 845-848.
14. Carmeliet P, Dor Y, Herbert JM, Fukumura D, Brusselmans K, et al. (1998) Role of HIF-1alpha in hypoxia-mediated apoptosis, cell proliferation and tumour angiogenesis. Nature 394: 485-490.
15. Wykoff CC, Beasley NJ, Watson PH, Turner KJ, Pastorek J, et al. (2000) Hypoxia-inducible expression of tumor-associated carbonic anhydrases. Cancer Res 60: 7075-7083.
16. Covello KL, Kehler J, Yu H, Gordan JD, Arsham AM, et al. (2006) HIF-2alpha regulates Oct-4: effects of hypoxia on stem cell function, embryonic development, and tumor growth. Genes Dev 20: 557-570.
17. Staab A, Loeffler J, Said HM, Diehlmann D, Katzer A, et al. (2007) Effects of HIF-1 inhibition by chetomin on hypoxia-related transcription and radiosensitivity in HT 1080 human fibrosarcoma cells. BMC Cancer 7: 213.
18. Wang Y, Liu Y, Malek SN, Zheng P, (2011) Targeting HIF1alpha Eliminates Cancer Stem Cells in Hematological Malignancies. Cell Stem Cell 8: 399-411.
19. Berchner-Pfannschmidt U, Frede S, Wotzlaw C, Fandrey J, (2008) Imaging of the hypoxia-inducible factor pathway: insights into oxygen sensing. Eur Respir J 32: 210-217.
20. Safran M, Kim WY, O'Connell F, Flippin L, Gunzler V, et al. (2006) Mouse model for noninvasive imaging of HIF prolyl hydroxylase activity: assessment of an oral agent that stimulates erythropoietin production. Proc Natl Acad Sci U S A 103: 105-110.
21. Shibata T, Akiyama N, Noda M, Sasai K, Hiraoka M, (1998) Enhancement of gene expression under hypoxic conditions using fragments of the human vascular endothelial growth factor and the erythropoietin genes. Int J Radiat Oncol Biol Phys 42: 913-916.
22. Post DE, Van Meir EG, (2001) Generation of bidirectional hypoxia/HIF-responsive expression vectors to target gene expression to hypoxic cells. Gene Ther 8: 1801-1807.
23. Biechele TL, Moon RT, (2008) Assaying beta-catenin/TCF transcription with beta-catenin/TCF transcription-based reporter constructs. Methods Mol Biol 468: 99-110.
24. Biechele TL, Adams AM, Moon RT, (2009) Transcription-based reporters of Wnt/beta-catenin signaling. Cold Spring Harb Protoc 2009: pdb prot5223.
25. Shibata T, Giaccia AJ, Brown JM, (2000) Development of a hypoxia-responsive vector for tumor-specific gene therapy. Gene Ther 7: 493-498.
26. Ghoda L, van Daalen Wetters T, Macrae M, Ascherman D, Coffino P, (1989) Prevention of rapid intracellular degradation of ODC by a carboxyl-terminal truncation. Science 243: 1493-1495.
27. Lee YM, Jeong CH, Koo SY, Son MJ, Song HS, et al. (2001) Determination of hypoxic region by hypoxia marker in developing mouse embryos in vivo: a possible signal for vessel development. Dev Dyn 220: 175-186.
28. Krishnan J, Ahuja P, Bodenmann S, Knapik D, Perriard E, et al. (2008) Essential role of developmentally activated hypoxia-inducible factor 1alpha for cardiac morphogenesis and function. Circ Res 103: 1139-1146.
29. Harvey RP, (2002) Patterning the vertebrate heart. Nat Rev Genet 3: 544-556.
30. Amarilio R, Viukov SV, Sharir A, Eshkar-Oren I, Johnson RS, et al. (2007) HIF1alpha regulation of Sox9 is necessary to maintain differentiation of hypoxic prechondrogenic cells during early skeletogenesis. Development 134: 3917-3928.
31. Provot S, Zinyk D, Gunes Y, Kathri R, Le Q, et al. (2007) Hif-1alpha regulates differentiation of limb bud mesenchyme and joint development. J Cell Biol 177: 451-464.
32. Schipani E, Ryan HE, Didrickson S, Kobayashi T, Knight M, et al. (2001) Hypoxia in cartilage: HIF-1alpha is essential for chondrocyte growth arrest and survival. Genes Dev 15: 2865-2876.
33. Schipani E, (2006) Hypoxia and HIF-1alpha in chondrogenesis. Ann N Y Acad Sci 1068: 66-73.
34. Macfarlane CM, (1997) In vitro influence of sublethal hypoxia on differentiation of the 3T3-L1 preadipose cell line and its physiological implications. Life Sci 60: 1923-1931.
35. Sahai A, Patel MS, Zavosh AS, Tannen RL, (1994) Chronic hypoxia impairs the differentiation of 3T3-L1 fibroblast in culture: role of sustained protein kinase C activation. J Cell Physiol 160: 107-112.
36. Modarressi A, Pietramaggiori G, Godbout C, Vigato E, Pittet B, et al. (2010) Hypoxia impairs skin myofibroblast differentiation and function. J Invest Dermatol 130: 2818-2827.
37. Kong X, Alvarez-Castelao B, Lin Z, Castano JG, Caro J, (2007) Constitutive/hypoxic degradation of HIF-alpha proteins by the proteasome is independent of von Hippel Lindau protein ubiquitylation and the transactivation activity of the protein. J Biol Chem 282: 15498-15505.
38. Mathieu J, Zhou W, Zhang Z, Wang AJ, Heddleston JM, et al. (2011) HIF induces human embryonic stem cell markers in cancer cells. Cancer Research 71: 4640-4652.
39. Schulte TW, Neckers LM, (1998) The benzoquinone ansamycin 17-allylamino-17-demethoxygeldanamycin binds to HSP90 and shares important biologic activities with geldanamycin. Cancer Chemother Pharmacol 42: 273-279.
40. Chiarotto JA, Hill RP, (1999) A quantitative analysis of the reduction in oxygen levels required to induce up-regulation of vascular endothelial growth factor (VEGF) mRNA in cervical cancer cell lines. Br J Cancer 80: 1518-1524.
41. Pilch H, Schlenger K, Steiner E, Brockerhoff P, Knapstein P, et al. (2001) Hypoxia-stimulated expression of angiogenic growth factors in cervical cancer cells and cervical cancer-derived fibroblasts. Int J Gynecol Cancer 11: 137-142.
42. Lin Z, Bazzaro M, Wang MC, Chan KC, Peng S, et al. (2009) Combination of proteasome and HDAC inhibitors for uterine cervical cancer treatment. Clin Cancer Res 15: 570-577.
43. Schwock J, Pham NA, Cao MP, Hedley DW, (2008) Efficacy of Hsp90 inhibition for induction of apoptosis and inhibition of growth in cervical carcinoma cells in vitro and in vivo. Cancer Chemother Pharmacol 61: 669-681.
44. Barbosa MS, Vass WC, Lowy DR, Schiller JT, (1991) In vitro biological activities of the E6 and E7 genes vary among human papillomaviruses of different oncogenic potential. J Virol 65: 292-298.
45. Nakamura M, Bodily JM, Beglin M, Kyo S, Inoue M, et al. (2009) Hypoxia-specific stabilization of HIF-1alpha by human papillomaviruses. Virology 387: 442-448.
46. Huh K, Zhou X, Hayakawa H, Cho JY, Libermann TA, et al. (2007) Human papillomavirus type 16 E7 oncoprotein associates with the cullin 2 ubiquitin ligase complex, which contributes to degradation of the retinoblastoma tumor suppressor. J Virol 81: 9737-9747.
47. Barry SC, Harder B, Brzezinski M, Flint LY, Seppen J, et al. (2001) Lentivirus vectors encoding both central polypurine tract and posttranscriptional regulatory element provide enhanced transduction and transgene expression. Hum Gene Ther 12: 1103-1108.
48. Yan Q, Bartz S, Mao M, Li L, Kaelin WG, (2007) The hypoxia-inducible factor 2alpha N-terminal and C-terminal transactivation domains cooperate to promote renal tumorigenesis in vivo. Mol Cell Biol 27: 2092-2102.
49. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, et al. (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3: RESEARCH0034.