Improved production of holotoxin Stx2 with biological activities by using a single-promoter vector and an auto-induction expression system

Protein Expression and Purification

Volumn 67, Issue 2, 2009, Pages 169-174

  Tu, Wei ^a   Cai, Kun ^a   Gao, Xiang ^a   Xiao, Le ^a   Chen, Rongchang ^a   Shi, Jing ^a   Liu, Hao ^a   Hou, Xiaojun ^a   Wang, Qin ^a   Wang, Hui ^a  

^a BEIJING INSTITUTE OF MICROBIOLOGY AND EPIDEMIOLOGY   (China)

Author keywords

Auto induction system; Recombinant Shiga toxin 2 (rStx2); Signal peptide; Single promoter

Indexed keywords

RECOMBINANT PROTEIN; SIGNAL PEPTIDE; VEROTOXIN 2;

ANIMAL; ARTICLE; BAGG ALBINO MOUSE; BIOSYNTHESIS; CELL SURVIVAL; CERCOPITHECUS; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FEMALE; GENE VECTOR; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOUSE; PROMOTER REGION; SEQUENCE ANALYSIS; SERODIAGNOSIS; VERO CELL;

ANIMALS; CELL SURVIVAL; CERCOPITHECUS AETHIOPS; CLONING, MOLECULAR; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FEMALE; GENETIC VECTORS; MICE; MICE, INBRED BALB C; NEUTRALIZATION TESTS; PROMOTER REGIONS, GENETIC; PROTEIN SORTING SIGNALS; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, PROTEIN; SHIGA TOXIN 2; VERO CELLS;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 67650421490     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.05.005     Document Type: Article

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