Split-Inteins for Simultaneous, site-specific conjugation of Quantum Dots to multiple protein targets In vivo

Journal of Nanobiotechnology

Volumn 9, Issue , 2011, Pages

  Charalambous, Anna ^a   Antoniades, Ioanna ^a   Christodoulou, Neophytos ^a   Skourides, Paris A ^a  

^a UNIVERSITY OF CYPRUS   (Cyprus)

Author keywords

[No Author keywords available]

Indexed keywords

BIO-IMAGING; BIOLOGICAL IMAGING; C-TERMINUS; FOCAL ADHESIONS; IN-VIVO; INTRACELLULAR COMPARTMENTS; LIVE CELL; MOLECULAR COMPLEXES; MULTIPARAMETERS; MULTIPLE TARGETS; N-TERMINALS; PAXILLIN; PROTEIN TAGGING; PROTEIN TARGETS; SITE-SPECIFIC; SPATIO-TEMPORAL DYNAMICS; SPLIT-INTEINS; TARGET PROTEINS; TEMPORAL RESOLUTION; UNIQUE FEATURES;

DISSOLUTION; PROTEINS;

SEMICONDUCTOR QUANTUM DOTS;

BIOTIN; ENHANCED GREEN FLUORESCENT PROTEIN; INTEIN; PAXILLIN; PROTEIN TAG; QUANTUM DOT; STREPTAVIDIN; DNA DIRECTED DNA POLYMERASE GAMMA; DNA POLYMERASE III, ALPHA SUBUNIT; DNAB HELICASE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; RNA; XENOPUS PROTEIN;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; BIOTINYLATION; CARBOXY TERMINAL SEQUENCE; CELL MEMBRANE; COMPLEX FORMATION; CONJUGATION; DISSOLUTION; EMBRYO; FOCAL ADHESION; IN VITRO STUDY; IN VIVO STUDY; MATERIAL COATING; NONHUMAN; PROTEIN LOCALIZATION; PROTEIN TARGETING; SIGNAL TRANSDUCTION; ANIMAL; ANIMAL EMBRYO; ENZYMOLOGY; GENETICS; METABOLISM; PRENATAL DEVELOPMENT; PROTEIN PROCESSING; XENOPUS;

ANIMALS; DNA POLYMERASE III; DNAB HELICASES; EMBRYO, NONMAMMALIAN; FOCAL ADHESIONS; GREEN FLUORESCENT PROTEINS; INTEINS; PAXILLIN; PROTEIN SPLICING; QUANTUM DOTS; RECOMBINANT FUSION PROTEINS; RNA; XENOPUS; XENOPUS PROTEINS;

EID: 80052867176     PISSN: None     EISSN: 14773155     Source Type: Journal    
DOI: 10.1186/1477-3155-9-37     Document Type: Article

References (60)

1. Finley KR, Davidson AE, Ekker SC. Three-color imaging using fluorescent proteins in living zebrafish embryos. Biotechniques 2001, 31:66.
2. Giuliano KA, Post PL, Hahn KM, Taylor DL. Fluorescent protein biosensors: measurement of molecular dynamics in living cells. Annu Rev Biophys Biomol Struct 1995, 24:405. 10.1146/annurev.bb.24.060195.002201, 7663122.
3. Giepmans BN, Adams SR, Ellisman MH, Tsien RY. The fluorescent toolbox for assessing protein location and function. Science 2006, 312:217. 10.1126/science.1124618, 16614209.
4. Bruchez M, Moronne M, Gin P, Weiss S, Alivisatos AP. Semiconductor nanocrystals as fluorescent biological labels. Science 1998, 281:2013.
5. Chan WC, Nie S. Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 1998, 281:2016.
6. Dahan M, et al. Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking. Science 2003, 302:442. 10.1126/science.1088525, 14564008.
7. Han M, Gao X, Su JZ, Nie S. Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules. Nat Biotechnol 2001, 19:631. 10.1038/90228, 11433273.
8. Larson DR, et al. Water-soluble quantum dots for multiphoton fluorescence imaging in vivo. Science 2003, 300:1434. 10.1126/science.1083780, 12775841.
9. Michalet X, et al. Quantum dots for live cells, in vivo imaging, and diagnostics. Science 2005, 307:538. 10.1126/science.1104274, 1201471, 15681376.
10. Courty S, Luccardini C, Bellaiche Y, Cappello G, Dahan M. Tracking individual kinesin motors in living cells using single quantum-dot imaging. Nano Lett 2006, 6:1491. 10.1021/nl060921t, 16834436.
11. Howarth M, et al. Monovalent, reduced-size quantum dots for imaging receptors on living cells. Nat Methods 2008, 5:397. 10.1038/nmeth.1206, 2637151, 18425138.
12. Smith AM, Nie S. Minimizing the hydrodynamic size of quantum dots with multifunctional multidentate polymer ligands. J Am Chem Soc 2008, 130:11278. 10.1021/ja804306c, 2703485, 18680294.
13. Medintz IL, et al. Self-assembled nanoscale biosensors based on quantum dot FRET donors. Nat Mater 2003, 2:630. 10.1038/nmat961, 12942071.
14. Howarth M, Takao K, Hayashi Y, Ting AY. Targeting quantum dots to surface proteins in living cells with biotin ligase. Proc Natl Acad Sci USA 2005, 102:7583. 10.1073/pnas.0503125102, 1129026, 15897449.
15. So MK, Yao H, Rao J. HaloTag protein-mediated specific labeling of living cells with quantum dots. Biochem Biophys Res Commun 2008, 374:419. 10.1016/j.bbrc.2008.07.004, 2553894, 18621022.
16. Colston MJ, Davis EO. The ins and outs of protein splicing elements. Mol Microbiol 1994, 12:359. 10.1111/j.1365-2958.1994.tb01025.x, 8065257.
17. Cooper AA, Stevens TH. Protein splicing: self-splicing of genetically mobile elements at the protein level. Trends Biochem Sci 1995, 20:351. 10.1016/S0968-0004(00)89075-1, 7482702.
18. Xu MQ, Perler FB. The mechanism of protein splicing and its modulation by mutation. Embo J 1996, 15:5146. 452257, 8895558.
19. Evans TJT, Xu MQ. Mechanistic and kinetic considerations of protein splicing. Chem Rev 2002, 102:4869. 10.1021/cr9601369, 12475209.
20. Giriat I, Muir TW. Protein semi-synthesis in living cells. J Am Chem Soc 2003, 125:7180. 10.1021/ja034736i, 12797783.
21. Muralidharan V, Muir TW. Protein ligation: an enabling technology for the biophysical analysis of proteins. Nat Methods 2006, 3:429. 10.1038/nmeth886, 16721376.
22. Chong S, et al. Protein splicing involving the Saccharomyces cerevisiae VMA intein. The steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system. J Biol Chem 1996, 271:22159. 10.1074/jbc.271.36.22159, 8703028.
23. Shao Y, Xu MQ, Paulus H. Protein splicing: evidence for an N-O acyl rearrangement as the initial step in the splicing process. Biochemistry 1996, 35:3810. 10.1021/bi952592h, 8620003.
24. Xu MQ, et al. Protein splicing: an analysis of the branched intermediate and its resolution by succinimide formation. Embo J 1994, 13:5517. 395513, 7988548.
25. Xu MQ, Southworth MW, Mersha FB, Hornstra LJ, Perler FB. In vitro protein splicing of purified precursor and the identification of a branched intermediate. Cell 1993, 75:1371. 10.1016/0092-8674(93)90623-X, 8269515.
26. Shao Y, Xu MQ, Paulus H. Protein splicing: characterization of the aminosuccinimide residue at the carboxyl terminus of the excised intervening sequence. Biochemistry 1995, 34:10844. 10.1021/bi00034a017, 7662664.
27. Lew BM, Mills KV, Paulus H. Protein splicing in vitro with a semisynthetic two-component minimal intein. J Biol Chem 1998, 273:15887. 10.1074/jbc.273.26.15887, 9632632.
28. Busche AE, et al. Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein. Angew Chem Int Ed Engl 2009, 48:6128. 10.1002/anie.200901488, 19591176.
29. Cowburn D, Shekhtman A, Xu R, Ottesen JJ, Muir TW. Segmental isotopic labeling for structural biological applications of NMR. Methods Mol Biol 2004, 278:47.
30. Zuger S, Iwai H. Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies. Nat Biotechnol 2005, 23:736. 10.1038/nbt1097, 15908942.
31. Xia Z, et al. Multiplex detection of protease activity with quantum dot nanosensors prepared by intein-mediated specific bioconjugation. Anal Chem 2008, 80:8649. 10.1021/ac801562f, 2677517, 18922019.
32. Evans TC, et al. Protein trans-splicing and cyclization by a naturally split intein from the dnaE gene of Synechocystis species PCC6803. J Biol Chem 2000, 275:9091. 10.1074/jbc.275.13.9091, 10734038.
33. Scott CP, Abel-Santos E, Wall M, Wahnon DC, Benkovic SJ. Production of cyclic peptides and proteins in vivo. Proc Natl Acad Sci USA 1999, 96:13638. 10.1073/pnas.96.24.13638, 24117, 10570125.
34. Charalambous A, Andreou M, Skourides PA. Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivo. J Nanobiotechnology 2009, 7:9. 10.1186/1477-3155-7-9, 2799375, 20003264.
35. Perler FB. InBase: the Intein Database. Nucleic Acids Res 2002, 30:383. 10.1093/nar/30.1.383, 99080, 11752343.
36. Saleh L, Perler FB. Protein splicing in cis and in trans. Chem Rec 2006, 6:183. 10.1002/tcr.20082, 16900466.
37. Wu H, Hu Z, Liu XQ. Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803. Proc Natl Acad Sci USA 1998, 95:9226. 10.1073/pnas.95.16.9226, 21320, 9689062.
38. Ludwig C, Pfeiff M, Linne U, Mootz HD. Ligation of a synthetic peptide to the N terminus of a recombinant protein using semisynthetic protein trans-splicing. Angew Chem Int Ed Engl 2006, 45:5218. 10.1002/anie.200600570, 16823791.
39. Ludwig C, Schwarzer D, Mootz HD. Interaction studies and alanine scanning analysis of a semi-synthetic split intein reveal thiazoline ring formation from an intermediate of the protein splicing reaction. J Biol Chem 2008, 283:25264. 10.1074/jbc.M802972200, 18625708.
40. Telenti A, et al. The Mycobacterium xenopi GyrA protein splicing element: characterization of a minimal intein. J Bacteriol 1997, 179:6378. 179553, 9335286.
41. Sun W, Yang J, Liu XQ. Synthetic two-piece and three-piece split inteins for protein trans-splicing. J Biol Chem 2004, 279:35281. 10.1074/jbc.M405491200, 15194682.
42. Volkmann G, Iwai H. Protein trans-splicing and its use in structural biology: opportunities and limitations. Mol Biosyst 6:2110.
43. Schaller MD. Paxillin: a focal adhesion-associated adaptor protein. Oncogene 2001, 20:6459. 10.1038/sj.onc.1204786, 11607845.
44. Stylianou P, Skourides PA. Imaging morphogenesis, in Xenopus with Quantum Dot nanocrystals. Mech Dev 2009,
45. Aranko AS, Zuger S, Buchinger E, Iwai H. In vivo and in vitro protein ligation by naturally occurring and engineered split DnaE inteins. PLoS One 2009, 4:e5185. 10.1371/journal.pone.0005185, 2664965, 19365564.
46. Jiang W, Hunter T. Analysis of cell-cycle profiles in transfected cells using a membrane-targeted GFP. Biotechniques 1998, 24:349.
47. Iwai H, Zuger S, Jin J, Tam PH. Highly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett 2006, 580:1853. 10.1016/j.febslet.2006.02.045, 16516207.
48. Dassa B, Amitai G, Caspi J, Schueler-Furman O, Pietrokovski S. Trans protein splicing of cyanobacterial split inteins in endogenous and exogenous combinations. Biochemistry 2007, 46:322. 10.1021/bi0611762, 17198403.
49. Mayor S, Riezman H. Sorting GPI-anchored proteins. Nat Rev Mol Cell Biol 2004, 5:110. 10.1038/nrm1309, 15040444.
50. Purdue PE, Lazarow PB. Peroxisome biogenesis. Annu Rev Cell Dev Biol 2001, 17:701. 10.1146/annurev.cellbio.17.1.701, 11687502.
51. Zhang FL, Casey PJ. Protein prenylation: molecular mechanisms and functional consequences. Annu Rev Biochem 1996, 65:241. 10.1146/annurev.bi.65.070196.001325, 8811180.
52. Lo SH. Focal adhesions: what's new inside. Dev Biol 2006, 294:280. 10.1016/j.ydbio.2006.03.029, 16650401.
53. Hynes RO. Integrins: bidirectional, allosteric signaling machines. Cell 2002, 110:673. 10.1016/S0092-8674(02)00971-6, 12297042.
54. Ridley AJ, et al. Cell migration: integrating signals from front to back. Science 2003, 302:1704. 10.1126/science.1092053, 14657486.
55. Smith AM, Gao X, Nie S. Quantum dot nanocrystals for in vivo molecular and cellular imaging. Photochem Photobiol 2004, 80:377.
56. Winklbauer R. Mesodermal cell migration during Xenopus gastrulation. Dev Biol 1990, 142:155. 10.1016/0012-1606(90)90159-G, 2227092.
57. Smith WC, Harland RM. Injected Xwnt-8 RNA acts early in Xenopus embryos to promote formation of a vegetal dorsalizing center. Cell 1991, 67:753. 10.1016/0092-8674(91)90070-F, 1657405.
58. Schnolzer M, Alewood P, Jones A, Alewood D, Kent SB. In situ neutralization in Boc-chemistry solid phase peptide synthesis. Rapid, high yield assembly of difficult sequences. Int J Pept Protein Res 1992, 40:180.
59. Stronati A, et al. Relationships between sperm DNA fragmentation, sperm apoptotic markers and serum levels of CB-153 and p,p'-DDE in European and Inuit populations. Reproduction 2006, 132:949. 10.1530/rep.1.01034, 17127755.
60. Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res 1976, 36:4562.