Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies

Nature Biotechnology

Volumn 23, Issue 6, 2005, Pages 736-740

  Züger, Sara ^a   Iwai, Hideo ^a  

^a UNIVERSITY OF SASKATCHEWAN   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSYNTHESIS; ESCHERICHIA COLI; ISOTOPES; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

BIOSYNTHETIC INCORPORATION; PROTEIN LIGATION; PROTEIN STABILITY;

PROTEINS;

INTEIN; CARBON; GUANINE NUCLEOTIDE BINDING PROTEIN; NITROGEN; RECOMBINANT PROTEIN;

ARTICLE; BIOSYNTHESIS; ESCHERICHIA COLI; IN VIVO STUDY; ISOTOPE LABELING; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN STABILITY; CHEMISTRY; GENE EXPRESSION; METABOLISM; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE; PLASMID;

ESCHERICHIA COLI;

CARBON ISOTOPES; ESCHERICHIA COLI; GENE EXPRESSION; GTP-BINDING PROTEINS; INTEINS; ISOTOPE LABELING; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PLASMIDS; RECOMBINANT PROTEINS;

EID: 24944448735     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt1097     Document Type: Article

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