Interaction studies and alanine scanning analysis of a semi-synthetic split intein reveal thiazoline ring formation from an intermediate of the protein splicing reaction

Journal of Biological Chemistry

Volumn 283, Issue 37, 2008, Pages 25264-25272

  Ludwig, Christina ^a   Schwarzer, Dirk ^a,b   Mootz, Henning D ^a  

^a TU DORTMUND UNIVERSITY   (Germany)

^b LEIBNIZ INSTITUT FÜR MOLEKULARE PHARMAKOLOGIE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMINATION; AMINES; AMINO ACIDS; CHEMICAL REACTIONS; ORGANIC ACIDS; SCANNING;

ACTIVE SITES; COMPLEX FORMATIONS; EXPERIMENTAL EVIDENCES; FLUORESCENCE ANISOTROPIES; FUNCTION RELATIONSHIPS; HYDROPHOBIC RESIDUES; INTERACTION STUDIES; RING FORMATIONS; SHIFT ANDS; SIDE REACTIONS; SPLICING REACTIONS; SYNTHETIC PROTEINS; TEMPORAL ALIGNMENTS;

PORT TERMINALS;

ALANINE; C TERMINAL INTEIN; CYSTEINE; INTEIN; N TERMINAL INTEIN; OXYTHIAZOLIDINE; THIAZOLIDINE DERIVATIVE; THIAZOLINE; UNCLASSIFIED DRUG; PEPTIDE; PROTEIN; THIAZOLE DERIVATIVE;

AMINO ACID SEQUENCE; ANISOTROPY; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; FLUORESCENCE ANALYSIS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN INTERACTION; PROTEIN PROCESSING; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; STRUCTURE ACTIVITY RELATION; ALTERNATIVE RNA SPLICING; CHEMISTRY; ESCHERICHIA COLI; KINETICS; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; PLASMID; PROTEIN TERTIARY STRUCTURE; SPECTROFLUOROMETRY;

ALANINE; ALTERNATIVE SPLICING; AMINO ACID SEQUENCE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; INTEINS; KINETICS; MOLECULAR SEQUENCE DATA; PEPTIDES; PLASMIDS; PROTEIN STRUCTURE, TERTIARY; PROTEINS; SPECTROMETRY, FLUORESCENCE; THIAZOLES; THIAZOLIDINES;

EID: 54449093009     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M802972200     Document Type: Article

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