RF cloning: A restriction-free method for inserting target genes into plasmids

Journal of Biochemical and Biophysical Methods

Volumn 67, Issue 1, 2006, Pages 67-74

  Van Den Ent, Fusinita ^a   Löwe, Jan ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

High throughput cloning; Ligation independent cloning; Restriction free cloning; Structural genomics

Indexed keywords

DNA FRAGMENT;

ARTICLE; GENE AMPLIFICATION; GENE DELETION; GENE INSERTION; GENE MUTATION; GENE TARGETING; GENOMICS; MOLECULAR CLONING; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION MAPPING; SITE DIRECTED MUTAGENESIS;

CELL LINE; CLONING, MOLECULAR; DNA PRIMERS; ESCHERICHIA COLI; GENETIC VECTORS; HUMANS; PLASMIDS; POLYMERASE CHAIN REACTION;

EID: 33645021327     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbbm.2005.12.008     Document Type: Article

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