Structural basis for inhibition of the replication licensing factor Cdt1 by geminin

Nature

Volumn 430, Issue 7002, 2004, Pages 913-917

  Lee, Changwook ^a   Hong, BumSoo ^a   Choi, Jung Min ^a   Kim, Yugene ^a   Watanabe, Saori ^b   Ishimi, Yukio ^c   Enomoto, Takemi ^b   Tada, Shusuke ^b   Kim, Youngchang ^d   Cho, Yunje ^a  

^a Pohang University of Science and Technology (POSTECH)   (South Korea)

^b TOHOKU UNIVERSITY   (Japan)

^c MITSUBISHI KAGAKU INSTITUTE OF LIFE SCIENCES   (Japan)

^d ARGONNE NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHROMOSOMES; CRYSTAL STRUCTURE; DATA ACQUISITION; DIMERS;

GEMININ; STERIC HINDRANCE;

CELL CULTURE;

ALANINE; CYCLIN DEPENDENT KINASE; DIMER; GEMININ; HELICASE; LEUCINE; MINICHROMOSOME MAINTENANCE PROTEIN; PHENYLALANINE; PROLINE; PROTEIN; REPLICATION LICENSING FACTOR CDT1; TYROSINE; UNCLASSIFIED DRUG;

BIOLOGY; CHROMOSOME;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL CYCLE; COMPLEX FORMATION; CRYSTAL STRUCTURE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN INTERACTION; PROTEIN STRUCTURE;

ANIMALS; BINDING SITES; CELL CYCLE PROTEINS; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; DNA REPLICATION; DNA-BINDING PROTEINS; MCM1 PROTEIN; MICE; MODELS, MOLECULAR; NUCLEAR PROTEINS; PEPTIDE FRAGMENTS; PROTEIN CONFORMATION; STRUCTURE-ACTIVITY RELATIONSHIP; XENOPUS; XENOPUS PROTEINS;

EUKARYOTA;

EID: 4344716063     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/nature02813     Document Type: Article

References (29)

1. Bell, S. P. & Dutta, A. DNA replication in eukaryotic cells. Annu. Rev. Biochem. 71, 333-374 (2002).
2. Blow, J. J. & Hodgson, B. Replication licensing-defining the proliferative state? Trends Cell Biol. 12, 72-78 (2002).
3. Diffley, J. F. DNA replication: building the perfect switch. Curr. Biol. 11, R367-R370 (2001).
4. Bell, S. P. & Stillman, B. ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex. Nature 357, 128-134 (1992).
5. Coleman, T. R., Carpenter, P. B. & Dunphy, W. G. The Xenopus Cdc6 protein is essential for the initiation of a single round of DNA replication in cell-free extracts. Cell 87, 53-63 (1996).
6. Nishitani, H., Lygerou, Z., Nishimoto, T. & Nurse, P. The Cdt1 protein is required to license DNA for replication in fission yeast. Nature 404, 625-628 (2000).
7. Shreeram, S. & Blow, J. J. The role of the replication licensing system in cell proliferation and cancer. Prog. Cell Cycle Res. 5, 287-293 (2003).
8. Vaziri, C. et al. A p53-dependent checkpoint pathway prevents rereplication. Mol. Cell 11, 997-1008 (2003).
9. Kearsey, S. E. & Cotterill, S. Enigmatic variations: divergent modes of regulating eukaryotic DNA replication. Mol. Cell 12, 1067-1075 (2003).
10. McGarry, T. J. & Kirschner, M. W. Geminin, an inhibitor of DNA replication, is degraded during mitosis. Cell 93, 1043-1053 (1998).
11. Tada, S., Li, A., Maiorano, D., Mechali, M. & Blow, J. J. Repression of origin assembly in metaphase depends on inhibition of RLF-B/Cdt1 by geminin. Nature Cell Biol. 3, 107-113 (2001).
12. Wohlschlegel, J. A. et al. Inhibition of eukaryotic DNA replication by geminin binding to Cdt1. Science 290, 2309-2312 (2000).
13. Yamaguchi, R. & Newport, J. A role for Ran-GTP and Crm1 in blocking re-replication. Cell 113, 115-125 (2003).
14. Li, A. & Blow, J. J. Non-proteolytic inactivation of geminin requires CDK-dependent ubiquitination. Nature Cell Biol. 6, 260-267 (2004).
15. Hofmann, J. F. & Beach, D. Cdt1 is an essential target of the Cdc10/Sct1 transcription factor: requirement for DNA replication and inhibition of mitosis. EMBO J. 13, 425-434 (1994).
16. Maiorano, D., Moreau, J. & Mechali, M. XCDT1 is required for the assembly of pre-replicative complexes in Xenopus laevis. Nature 404, 622-625 (2000).
17. Whittaker, A. J., Royzman, I. & Orr-Weaver, T. L. Drosophila double parked: a conserved, essential replication protein that colocalizes with the origin recognition complex and links DNA replication with mitosis and the down-regulation of S phase transcripts. Genes Dev. 14, 1765-1776 (2000).
18. Arentson, E. et al. Oncogenic potential of the DNA replication licensing protein CDT1. Oncogene 21, 1150-1158 (2002).
19. Yanagi, K., Mizuno, T., You, Z. & Hanaoka, F. Mouse geminin inhibits not only Cdt1-MCM6 interactions but also a novel intrinsic Cdt1 DNA binding activity. J. Biol. Chem. 277, 40871-40880 (2002).
20. Cook, J. G., Chasse, D. A. & Nevins, J. R. The regulated association of Cdt1 with minichromosome maintenance proteins and Cdc6 in mammalian cells. J. Biol. Chem. 279, 9625-9633 (2004).
21. Bussiere, D. E., Bastia, D. & White, S. W. Crystal structure of the replication terminator protein from B. subtilis at 2.6 Å. Cell 80, 651-660 (1995).
22. Gautam, A., Mulugu, S., Alexander, K. & Bastia, D. A single domain of the replication termination protein of Bacillus subtilis is involved in arresting both DnaB helicase and RNA polymerase. J. Biol. Chem. 276, 23471-23479 (2001).
23. Tanaka, S. & Diffley, J. F. Interdependent nuclear accumulation of budding yeast Cdt1 and Mcm2-7 during G1 phase. Nature Cell Biol. 4, 198-207 (2002).
24. O'Shea, E. K., Klemm, J. D., Kim, P. S. & Alber, T. X-ray structure of the GCN4 leucine zipper, a two-stranded, parallel coiled coil. Science 254, 539-544 (1991).
25. Shreeram, S., Sparks, A., Lane, D. P. & Blow, J. J. Cell type-specific responses of human cells to inhibition of replication licensing. Oncogene 21, 6624-6632 (2002).
26. Otwinowski, Z. & Minor, W. Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol. 276, 307-326 (1997).
27. Brünger, A. T. et al. Crystallography and NMR system: A new software suite for macromolecular structure determination. Acta Crystallogr. D 54, 905-921 (1998).
28. Kleywegt, G. J. & Jones, T. A. Efficient rebuilding of protein structures. Acta Crystallogr. D 50, 829-832 (1996).
29. You, Z., Komamura, Y. & Ishimi, Y. Biochemical analysis of the intrinsic Mcm4-Mcm6-Mcm7 DNA helicase activity. Mol. Cell. Biol. 12, 8003-8015 (1999).