Dissecting the interaction network of multiprotein complexes by pairwise coexpression of subunits in E. coli

Journal of Molecular Biology

Volumn 306, Issue 2, 2001, Pages 363-373

  Fribourg, Sébastien ^a   Romier, Christophe ^a   Werten, Sebastiaan ^a   Gangloff, Yann Gaël ^a   Poterszman, Arnaud ^a   Moras, Dino ^a  

^a PSL Research University   (France)

Author keywords

Interaction mapping; Over expression; Structural biology; TFIID; TFIIH

Indexed keywords

POLYPEPTIDE; PROTEIN SUBUNIT; TRANSCRIPTION FACTOR;

ARTICLE; COMPLEX FORMATION; ESCHERICHIA COLI; IMMUNOPRECIPITATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PROTEIN INTERACTION; PROTEIN STABILITY; PROTEIN STRUCTURE; SOLUBILITY;

EID: 0035895435     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.4376     Document Type: Article

References (31)

1. Analysis of TFIIH subunit through isolation of the gene from Schizosaccharomyces pombe corresponding to that of Saccharomyces cerevisiae SSL1, reveals the presence of conserved structural motifs
2. Three-dimensional structure of the human TFIID-IIA-IIB complex
3. The crystal structure of human cyclin H
4. Recombinant protein expression in Escherichia coli
5. Regulation of gene expression by multiple forms of TFIID and other novel TAFII-containing complexes
6. Human TAF(II)28 and TAF(II)18 interact through a historic fold encoded by atypical evolutionary conserved motifs also found in the SPT3 family
7. Three-dimensional structures of the TAFII-containing complexes TFIID and TFTC
8. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the p15A cryptic miniplamis
9. Subunit interactions in yeast Transcription/Repair factor TFIIH. Requirement for tfb3 subunit in nucleotide excision repair
10. Structural characterization of the cysteine-rich domain of TFIIH p44 subunit
11. The human TFIID components TAF(II)135 and TAF(II)20 and the yeast SAGA components ADA1 and TAF(II)68 heterodimerize to form histone-like pairs
12. Molecular genetics of the RNA polymerase II general transcriptional machinery
13. Escherichia coli skp chaperone coexpression improves solubility and phage display of single-chain antibody fragments
14. Recombinant replication protein A: Expression, complex formation, and functional characterization
15. Cloning and characterization of human TAF20/15. Multiple interactions suggest a central role in TFIID complex formation
16. p44 and p34 subunits of the BTF2/TFIIH transcription factor have homologies with SSL1, a yeast protein involved in DNA repair
17. Interactions involving the human RNA polymerase II transcription/nucleotide excision repair complex TFIIH, the nucleotide excision repair protein XPG, and Cockayne syndrome group B (CSB) protein
18. Structure and function of a human TAFII250 double bromodomain module
19. Coexpression of nuclear receptor partners increases their solubility and biological activities
20. Solution structure of a TBP-TAF(II)230 complex: Protein mimicry of the minor groove surface of the TATA box unwound by TBP
21. Crystal structure of the nucleosome core particle at 2.8 Å resolution
22. RNA polymerase II transcription initiation: A structural view
23. Structural basis of Rab effector specificity: Crystal structure of the small G protein Rab3A complexed with the effector domain of rabphilin-3A
24. Molecular structure of human TFIIH
25. Assembly of a functional immunoglobulin Fv fragment in Escherichia coli
26. The multiple roles of transcription/repair factor TFIIH
27. Cloning of the gene for inorganic pyrophosphatase from a thermoacidophilic archaeon, Sulfolobus sp. strain 7, and overproduction of the enzyme by coexpression of tRNA for arginine rare codon
28. Optimization of heterologous protein production in Escherichia coli
29. Heterologous expression in Escherichia coli of soluble active-site random mutants of haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 by coexpression of molecular chaperonins GroEL/ES
30. Structural similarity between TAFs and the heterotetrameric core of the histone octamer
31. Functional coexpression of serine protein kinase SRPKl and its substrate ASF/SF2 in Escherichia coli