Over-producing soluble protein complex and validating protein-protein interaction through a new bacterial co-expression system

Protein Expression and Purification

Volumn 69, Issue 1, 2010, Pages 47-53

  Zeng, Jumei ^a   Zhang, Lei ^a   Li, Yuqing ^a   Wang, Yi ^a   Wang, Mingchao ^a   Duan, Xin ^a   He, Zheng Guo ^a  

^a National Key Laboratory of Agricultural Microbiology   (China)

Author keywords

Co expression; Mycobacterium tuberculosis; pHEX; Protein complex

Indexed keywords

CARRIER PROTEIN; GLUTATHIONE TRANSFERASE; MALTOSE BINDING PROTEIN; MALTOSE-BINDING PROTEIN; MULTIPROTEIN COMPLEX;

ARTICLE; BIOCHEMISTRY; BIOSYNTHESIS; ESCHERICHIA COLI; GENE VECTOR; GENETICS; HUMAN; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MYCOBACTERIUM TUBERCULOSIS; PLASMID; PROTEIN BINDING; PROTEIN MULTIMERIZATION; REPRODUCIBILITY; SOLUBILITY; TWO HYBRID SYSTEM;

BIOCHEMISTRY; CARRIER PROTEINS; CLONING, MOLECULAR; ESCHERICHIA COLI; GENETIC VECTORS; GLUTATHIONE TRANSFERASE; HUMANS; MULTIPROTEIN COMPLEXES; MYCOBACTERIUM TUBERCULOSIS; PLASMIDS; PROTEIN BINDING; PROTEIN MULTIMERIZATION; REPRODUCIBILITY OF RESULTS; SOLUBILITY; TWO-HYBRID SYSTEM TECHNIQUES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MYCOBACTERIUM TUBERCULOSIS;

EID: 70350569294     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.09.002     Document Type: Article

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