An improved method for fast, robust, and seamless integration of DNA fragments into multiple plasmids

Protein Expression and Purification

Volumn 45, Issue 1, 2006, Pages 66-71

  Benoit, Roger M ^a   Wilhelm, Ramon N ^a   Scherer Becker, Daniela ^a   Ostermeier, Christian ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Heterologous protein expression; Homologous recombination; In Fusion; Seamless cloning

Indexed keywords

DNA; DNA FRAGMENT; HYBRID PROTEIN;

ARTICLE; CHEMISTRY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETICS; IN VITRO STUDY; ISOLATION AND PURIFICATION; MOLECULAR CLONING; PLASMID; POLYMERASE CHAIN REACTION;

CLONING, MOLECULAR; DNA; DNA FRAGMENTATION; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; PLASMIDS; POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI;

EID: 28844450410     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.09.022     Document Type: Article

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