메뉴 건너뛰기




Volumn 172, Issue 1, 2010, Pages 3-13

High-throughput production of human proteins for crystallization: The SGC experience

Author keywords

Ligation independent cloning; Open source; Protein expression; Protein purification; Structural genomics; Structure prediction

Indexed keywords

PROTEIN KINASE; PROTEIN TYROSINE PHOSPHATASE;

EID: 77956181585     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2010.06.008     Document Type: Article
Times cited : (254)

References (42)
  • 1
    • 20444474992 scopus 로고    scopus 로고
    • Protein structures hint at the shape of things to come
    • Abbott A. Protein structures hint at the shape of things to come. Nature 2005, 435:547.
    • (2005) Nature , vol.435 , pp. 547
    • Abbott, A.1
  • 2
    • 0025085655 scopus 로고
    • Ligation-independent cloning of PCR products (LIC-PCR)
    • Aslanidis C., de Jong P.J. Ligation-independent cloning of PCR products (LIC-PCR). Nucleic Acids Res. 1990, 18:6069-6074.
    • (1990) Nucleic Acids Res. , vol.18 , pp. 6069-6074
    • Aslanidis, C.1    de Jong, P.J.2
  • 6
    • 33749005086 scopus 로고    scopus 로고
    • In vivo and in vitro protein solubility assays using split GFP
    • Cabantous S., Waldo G.S. In vivo and in vitro protein solubility assays using split GFP. Nat. Methods 2006, 3:845-854.
    • (2006) Nat. Methods , vol.3 , pp. 845-854
    • Cabantous, S.1    Waldo, G.S.2
  • 7
    • 33748989829 scopus 로고    scopus 로고
    • An efficient and generic strategy for producing soluble human proteins and domains in E. coli by screening construct libraries
    • Cornvik T., Dahlroth S.L., Magnusdottir A., Flodin S., Engvall B., Lieu V., Ekberg M., Nordlund P. An efficient and generic strategy for producing soluble human proteins and domains in E. coli by screening construct libraries. Proteins 2006, 65:266-273.
    • (2006) Proteins , vol.65 , pp. 266-273
    • Cornvik, T.1    Dahlroth, S.L.2    Magnusdottir, A.3    Flodin, S.4    Engvall, B.5    Lieu, V.6    Ekberg, M.7    Nordlund, P.8
  • 9
    • 67650437045 scopus 로고    scopus 로고
    • Large-scale structural biology of the human proteome
    • Edwards A. Large-scale structural biology of the human proteome. Annu. Rev. Biochem. 2009, 78:541-568.
    • (2009) Annu. Rev. Biochem. , vol.78 , pp. 541-568
    • Edwards, A.1
  • 10
    • 67650506936 scopus 로고    scopus 로고
    • Open access chemical and clinical probes to support drug discovery
    • Edwards A.M., Bountra C., Kerr D.J., Willson T.M. Open access chemical and clinical probes to support drug discovery. Nat. Chem. Biol. 2009, 5:436-440.
    • (2009) Nat. Chem. Biol. , vol.5 , pp. 436-440
    • Edwards, A.M.1    Bountra, C.2    Kerr, D.J.3    Willson, T.M.4
  • 17
    • 33644874198 scopus 로고    scopus 로고
    • Combinatorial library approaches for improving soluble protein expression in Escherichia coli
    • Hart D.J., Tarendeau F. Combinatorial library approaches for improving soluble protein expression in Escherichia coli. Acta Crystallogr. D Biol. Crystallogr. 2006, 62:19-26.
    • (2006) Acta Crystallogr. D Biol. Crystallogr. , vol.62 , pp. 19-26
    • Hart, D.J.1    Tarendeau, F.2
  • 18
    • 34447617851 scopus 로고    scopus 로고
    • Z(basic)-a novel purification tag for efficient protein recovery
    • Hedhammar M., Hober S. Z(basic)-a novel purification tag for efficient protein recovery. J. Chromatogr. A 2007, 1161:22-28.
    • (2007) J. Chromatogr. A , vol.1161 , pp. 22-28
    • Hedhammar, M.1    Hober, S.2
  • 19
    • 0033578684 scopus 로고    scopus 로고
    • Protein secondary structure prediction based on position-specific scoring matrices
    • Jones D.T. Protein secondary structure prediction based on position-specific scoring matrices. J. Mol. Biol. 1999, 292:195-202.
    • (1999) J. Mol. Biol. , vol.292 , pp. 195-202
    • Jones, D.T.1
  • 20
    • 0030005755 scopus 로고    scopus 로고
    • The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression
    • Lennon G., Auffray C., Polymeropoulos M., Soares M.B. The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression. Genomics 1996, 33:151-152.
    • (1996) Genomics , vol.33 , pp. 151-152
    • Lennon, G.1    Auffray, C.2    Polymeropoulos, M.3    Soares, M.B.4
  • 21
    • 0042622240 scopus 로고    scopus 로고
    • GlobPlot: exploring protein sequences for globularity and disorder
    • Linding R., Russell R.B., Neduva V., Gibson T.J. GlobPlot: exploring protein sequences for globularity and disorder. Nucleic Acids Res. 2003, 31:3701-3708.
    • (2003) Nucleic Acids Res. , vol.31 , pp. 3701-3708
    • Linding, R.1    Russell, R.B.2    Neduva, V.3    Gibson, T.J.4
  • 22
    • 0001641514 scopus 로고
    • Mutations of Bacteria from Virus Sensitivity to Virus Resistance
    • Luria S.E., Delbruck M. Mutations of Bacteria from Virus Sensitivity to Virus Resistance. Genetics 1943, 28:491-511.
    • (1943) Genetics , vol.28 , pp. 491-511
    • Luria, S.E.1    Delbruck, M.2
  • 23
    • 67649625535 scopus 로고    scopus 로고
    • Enabling IMAC purification of low abundance recombinant proteins from E. coli lysates
    • Magnusdottir A., Johansson I., Dahlgren L.G., Nordlund P., Berglund H. Enabling IMAC purification of low abundance recombinant proteins from E. coli lysates. Nat. Methods 2009, 6:477-478.
    • (2009) Nat. Methods , vol.6 , pp. 477-478
    • Magnusdottir, A.1    Johansson, I.2    Dahlgren, L.G.3    Nordlund, P.4    Berglund, H.5
  • 24
    • 3242887157 scopus 로고    scopus 로고
    • CD-Search: protein domain annotations on the fly
    • Marchler-Bauer A., Bryant S.H. CD-Search: protein domain annotations on the fly. Nucleic Acids Res. 2004, 32:W327-W331.
    • (2004) Nucleic Acids Res. , vol.32
    • Marchler-Bauer, A.1    Bryant, S.H.2
  • 25
    • 0038634975 scopus 로고    scopus 로고
    • Improvement of the GenTHREADER method for genomic fold recognition
    • McGuffin L.J., Jones D.T. Improvement of the GenTHREADER method for genomic fold recognition. Bioinformatics 2003, 19:874-881.
    • (2003) Bioinformatics , vol.19 , pp. 874-881
    • McGuffin, L.J.1    Jones, D.T.2
  • 26
    • 0034044314 scopus 로고    scopus 로고
    • The PSIPRED protein structure prediction server
    • McGuffin L.J., Bryson K., Jones D.T. The PSIPRED protein structure prediction server. Bioinformatics 2000, 16:404-405.
    • (2000) Bioinformatics , vol.16 , pp. 404-405
    • McGuffin, L.J.1    Bryson, K.2    Jones, D.T.3
  • 28
    • 12344291243 scopus 로고    scopus 로고
    • Hsp90 and Cdc37-a chaperone cancer conspiracy
    • Pearl L.H. Hsp90 and Cdc37-a chaperone cancer conspiracy. Curr. Opin. Genet. Dev. 2005, 15:55-61.
    • (2005) Curr. Opin. Genet. Dev. , vol.15 , pp. 55-61
    • Pearl, L.H.1
  • 30
    • 33846057724 scopus 로고    scopus 로고
    • NCBI reference sequences (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins
    • Pruitt K.D., Tatusova T., Maglott D.R. NCBI reference sequences (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins. Nucleic Acids Res. 2007, 35:D61-D65.
    • (2007) Nucleic Acids Res. , vol.35
    • Pruitt, K.D.1    Tatusova, T.2    Maglott, D.R.3
  • 33
    • 0032568655 scopus 로고    scopus 로고
    • SMART, a simple modular architecture research tool: identification of signaling domains
    • Schultz J., Milpetz F., Bork P., Ponting C.P. SMART, a simple modular architecture research tool: identification of signaling domains. Proc. Natl. Acad. Sci. USA 1998, 95:5857-5864.
    • (1998) Proc. Natl. Acad. Sci. USA , vol.95 , pp. 5857-5864
    • Schultz, J.1    Milpetz, F.2    Bork, P.3    Ponting, C.P.4
  • 34
    • 84934439701 scopus 로고    scopus 로고
    • Baculovirus expression vector system: an emerging host for high-throughput eukaryotic protein expression
    • Shrestha B., Smee C., Gileadi O. Baculovirus expression vector system: an emerging host for high-throughput eukaryotic protein expression. Methods Mol. Biol. 2008, 439:269-289.
    • (2008) Methods Mol. Biol. , vol.439 , pp. 269-289
    • Shrestha, B.1    Smee, C.2    Gileadi, O.3
  • 36
    • 0036926615 scopus 로고    scopus 로고
    • A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site
    • Stols L., Gu M., Dieckman L., Raffen R., Collart F.R., Donnelly M.I. A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site. Protein Expr. Purif. 2002, 25:8-15.
    • (2002) Protein Expr. Purif. , vol.25 , pp. 8-15
    • Stols, L.1    Gu, M.2    Dieckman, L.3    Raffen, R.4    Collart, F.R.5    Donnelly, M.I.6
  • 38
    • 53249084008 scopus 로고    scopus 로고
    • A pilot project to generate affinity reagents to human proteins
    • Uhlen M., Graslund S., Sundstrom M. A pilot project to generate affinity reagents to human proteins. Nat. Methods 2008, 5:854-855.
    • (2008) Nat. Methods , vol.5 , pp. 854-855
    • Uhlen, M.1    Graslund, S.2    Sundstrom, M.3
  • 41
    • 0033767942 scopus 로고    scopus 로고
    • Creating a structural genomics consortium
    • Williamson A.R. Creating a structural genomics consortium. Nat. Struct. Biol. 2000, 7(Suppl.):953.
    • (2000) Nat. Struct. Biol. , vol.7 , Issue.SUPPL. , pp. 953
    • Williamson, A.R.1
  • 42
    • 24644450812 scopus 로고    scopus 로고
    • Organic osmolytes as compatible, metabolic and counteracting cytoprotectants in high osmolarity and other stresses
    • Yancey P.H. Organic osmolytes as compatible, metabolic and counteracting cytoprotectants in high osmolarity and other stresses. J. Exp. Biol. 2005, 208:2819-2830.
    • (2005) J. Exp. Biol. , vol.208 , pp. 2819-2830
    • Yancey, P.H.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.