Small interfering RNA targeting HIF-1α reduces hypoxia-dependent transcription and radiosensitizes hypoxic HT 1080 human fibrosarcoma cells in vitro

Strahlentherapie und Onkologie

Volumn 187, Issue 4, 2011, Pages 252-259

  Staab, Adrian ^a,d   Fleischer, Markus ^a   Loeffler, Juergen ^a   Said, Harun M ^a   Katzer, Astrid ^a   Plathow, Christian ^b   Einsele, Herrmann ^a   Flentje, Michael ^a   Vordermark, Dirk ^a,c  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b UNIVERSITY OF FREIBURG   (Germany)

^c MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^d PAUL SCHERRER INSTITUTE   (Switzerland)

Author keywords

Hypoxia; Hypoxia inducible factor 1 ; Oxygen; Radiation; Small interfering RNA

Indexed keywords

BETA TUBULIN; HYPOXIA INDUCIBLE FACTOR 1ALPHA; MESSENGER RNA; SMALL INTERFERING RNA;

ARTICLE; CANCER RADIOTHERAPY; CELL HYPOXIA; CELL SURVIVAL; CLONOGENESIS; CONTROLLED STUDY; FIBROSARCOMA; GENE EXPRESSION; GENE SEQUENCE; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; OXYGEN TENSION; QUANTITATIVE ANALYSIS; RADIOSENSITIVITY; RADIOSENSITIZATION; REAL TIME POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

CELL HYPOXIA; CELL LINE, TUMOR; CELL SURVIVAL; DOSE-RESPONSE RELATIONSHIP, RADIATION; FIBROSARCOMA; GENE SILENCING; HUMANS; HYPOXIA-INDUCIBLE FACTOR 1, ALPHA SUBUNIT; RADIATION TOLERANCE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TRANSCRIPTION, GENETIC; TUMOR CELLS, CULTURED; TUMOR STEM CELL ASSAY;

EID: 79955741602     PISSN: 01797158     EISSN: 1439099X     Source Type: Journal    
DOI: 10.1007/s00066-011-2167-0     Document Type: Article

References (39)

1. D.M. Aebersold P. Burri K.T. Beer 2001 Expression of hypoxia-inducible factor-1alpha: a novel predictive and prognostic parameter in the radiotherapy of oropharyngeal cancer Cancer Res 61 2911 6 11306467 1:CAS:528: DC%2BD3MXivFGqsr8%3D (Pubitemid 32691934)
2. M. Bache M. Kappler H.M. Said 2008 Detection and specific targeting of hypoxic regions within solid tumors: current preclinical and clinical strategies Curr Med Chem 15 322 38 18288988 10.2174/092986708783497391 1:CAS:528:DC%2BD1cXnsVyis7o%3D (Pubitemid 351266939)
3. M. Balducci G. Apicella S. Manfrida 2010 Single-arm phase II study of conformal radiation therapy and temozolomide plus fractionated stereotactic conformal boost in high-grade gliomas: final report Strahlenther Onkol 186 558 64 20936460 10.1007/s00066-010-2101-x
4. K.M. Comerford E.P. Cummins C.T. Taylor 2004 c-Jun NH2-Terminal kinase activation contributes to hypoxia-inducible factor 1a-dependent Pglycoprotein expression in hypoxia Cancer Res 15 9057 61 10.1158/0008-5472.CAN-04-1919 (Pubitemid 39665517)
5. K.M. Comerford T.J. Wallace J. Karhausen 2002 Hypoxia-inducible factor-1-dependent regulation of the multidrug resistance (MDR1) gene Cancer Res 15 3387 94 (Pubitemid 34651384)
6. K. Dellas M. Bache S.U. Pigorsch 2008 Prognostic impact of HIF-1alpha expression in patients with definitive radiotherapy for cervical cancer Strahlenther Onkol 184 169 74 18330514 10.1007/s00066-008-1764-z (Pubitemid 351365780)
7. A.C. Epstein J.M. Gleadle L.A. McNeill 2001 C. elegans EGL-9 and mammalian homologs define a family of dioxygenases that regulate HIF by prolyl hydroxylation Cell 107 43 54 11595184 10.1016/S0092-8674(01)00507-4 1:CAS:528:DC%2BD3MXnsFOntL0%3D (Pubitemid 32972036)
8. D.L. Gillespie K. Whang B.T. Ragel 2007 Silencing of hypoxia-inducible factor-1alpha by RNA interference attenuates human glioma cell growth in vivo Clin Cancer Res 13 2441 8 17438103 10.1158/1078-0432.CCR-06-2692 1:CAS:528:DC%2BD2sXkt1enu7g%3D (Pubitemid 46698595)
9. E.A. Griffiths S.A. Pritchard H.R. Valentine 2007 Hypoxia-inducible factor-1alpha expression in the gastric carcinogenesis sequence and its prognostic role in gastric and gastro-oesophageal adenocarcinomas Br J Cancer 96 95 103 17179985 10.1038/sj.bjc.6603524 1:CAS:528:DC%2BD2sXis1Kgug%3D%3D (Pubitemid 46094645)
10. Hall, EJ. Radiobiology for the radiologist. 4. ed. Philadelphia: Lippincott; 1994
11. A.L. Harris 2002 Hypoxia-a key regulatory factor in tumour growth Nature Reviews Cancer 2 38 47 11902584 10.1038/nrc704 1:CAS:528:DC%2BD38XhvF2jtL4%3D (Pubitemid 37328806)
12. M. Ivan K. Kondo H. Yang 2001 HIF alpha targeted for VHL-mediated destruction by proline hydroxylation: implications for O2 sensing Science 292 464 8 11292862 10.1126/science.1059817 1:CAS:528:DC%2BD3MXjtVentbw%3D (Pubitemid 32335941)
13. P. Jaakkola D.R. Mole Y.M. Tian 2001 Targeting of HIF-alpha to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation Science 292 468 72 11292861 10.1126/science.1059796 1:CAS:528: DC%2BD3MXjtVentb0%3D (Pubitemid 32335942)
14. Kappler H. Taubert H.J. Holzhausen 2008 Immunohistochemical detection of HIF-1alpha and CAIX in advanced head-and-neck cancer. Prognostic role and correlation with tumor markers and tumor oxygenation parameters Strahlenther Onkol 184 393 9 18956515 10.1007/s00066-008-1813-7
15. Z.A. Khan S. Chakrabarti 2007 Cellular signaling and potential new treatment targets in diabetic retinopathy Experimental Diabetes Research 2007 31867 18288248 10.1155/2007/31867
16. A.L. Kung S.D. Zabludoff D.S. France 2004 Small molecule blockade of transcriptional coactivation of the hypoxia-inducible factor pathway Cancer Cell 6 33 43 15261140 10.1016/j.ccr.2004.06.009 1:CAS:528:DC%2BD2cXmtlKnt7s%3D (Pubitemid 38903160)
17. Lau SK, Boutros PC, Pintilie M, et al. Three-gene prognostic classifier for early-stage non small-cell lung cancer. J Clin Oncol 2007;25:5562-9.
18. J. Lövey I. Kenessey E. Raso 2007 Human recombinant erythropoietin-alpha increases the efficacy of irradiation in preclinical model Magy Onkol 51 53 61 17417676
19. R. Malhotra D.W. Tyson H.M. Rosevear 2008 Hypoxia-inducible factor-1alpha is a critical mediator of hypoxia induced apoptosis in cardiac H9c2 and kidney epithelial HK-2 cells BMC Cardiovascular Disorders 8 9 18447926 10.1186/1471-2261-8-9 (Pubitemid 351715990)
20. T. Matsuyama K. Nakanishi T. Hayashi 2005 Expression of hypoxia-inducible factor-1alpha in esophageal squamous cell carcinoma Cancer Sci 96 176 82 15771621 10.1111/j.1349-7006.2005.00025.x 1:CAS:528:DC%2BD2MXjsFWgtL4%3D (Pubitemid 40575919)
21. B.J. Moeller M.W. Dewhirst 2006 HIF-1 and tumour radiosensitivity Br J Cancer 95 1 5 16735998 10.1038/sj.bjc.6603201 1:CAS:528:DC%2BD28XmtFyrtLw%3D (Pubitemid 43980546)
22. G.L. Semenza 2007 Evaluation of HIF-1 inhibitors as anticancer agents Drug Discov. Today 12 853 9 17933687 10.1016/j.drudis.2007.08.006 1:CAS:528:DC%2BD2sXhtFKit77O (Pubitemid 47554628)
23. T. Shibata A.J. Giaccia J.M. Brown 2000 Development of a hypoxia-responsive vector for tumor-specific gene therapy Gene Ther 7 493 8 10757022 10.1038/sj.gt.3301124 1:CAS:528:DC%2BD3cXhvVahsbc%3D (Pubitemid 30156563)
24. B.S. Sorensen J. Alsner J. Overgaard 2007 Hypoxia induced expression of endogenous markers in vitro is highly influenced by pH Radiother Oncol 83 362 6 17512623 10.1016/j.radonc.2007.04.028 (Pubitemid 46907983)
25. A. Staab J. Loeffler H.M. Said 2007 Effects of HIF-1 inhibition by chetomin on hypoxia-related transcription and radiosensitivity in HT 1080 human fibrosarcoma cells BMC Cancer 7 213 17999771 10.1186/1471-2407-7-213
26. A. Staab J. Loffler H.M. Said A. Katzer 2007 Modulation of glucose metabolism inhibits hypoxic accumulation of Hypoxia-inducible factor-1a (HIF-1alpha) Strahlenther Onkol 183 366 73 17609869 10.1007/s00066-007-1649-6 (Pubitemid 47036700)
27. Y. Takahashi M. Nishikawa Y. Takakura 2008 Inhibition of tumor cell growth in the liver by RNA interference-mediated suppression of HIF-1alpha expression in tumor cells and hepatocytes Gene Ther 15 572 82 18273056 10.1038/sj.gt.3303103 1:CAS:528:DC%2BD1cXktVGqtb8%3D
28. D. Tinkl G.G. Grabenbauer H. Golcher 2009 Downstaging of pancreatic carcinoma after neoadjuvant chemoradiation Strahlenther Onkol 185 557 66 19756421 10.1007/s00066-009-1977-9
29. C. Trastour E. Benizri F. Ettore 2007 HIF-1alpha and CA IX staining in invasive breast carcinomas: prognosis and treatment outcome Int J Cancer 120 1451 8 17245699 10.1002/ijc.22436 1:CAS:528:DC%2BD2sXis12is7Y%3D (Pubitemid 46355379)
30. P. Vaupel A. Mayer 2007 Hypoxia in cancer: significance and impact on clinical outcome Cancer Metastasis Rev 26 225 39 17440684 10.1007/s10555-007- 9055-1 1:CAS:528:DC%2BD2sXotFWrtLo%3D (Pubitemid 47101660)
31. D. Vordermark A. Katzer K. Baier 2004 M. Cell type-specific association of hypoxia-inducible factor-1 alpha (HIF-1 alpha) protein accumulation and radiobiologic tumor hypoxia Int J Radiat Oncol Biol Phys 58 1242 50 15001269 10.1016/j.ijrobp.2003.11.030 1:CAS:528:DC%2BD2cXhslWmuro%3D (Pubitemid 38314810)
32. D. Vordermark T. Shibata J.M. Brown 2001 Green fluorescent protein is a suitable reporter of tumor hypoxia despite an oxygen requirement for chromophore formation Neoplasia 3 527 34 11774035 10.1038/sj.neo.7900192 1:CAS:528:DC%2BD38XksFKjtA%3D%3D (Pubitemid 33151019)
33. D. Vordermark J.M. Brown 2003 Endogenous markers of tumor hypoxia predictors of clinical radiation resistance? Strahlenther Onkol 179 801 11 14652668 10.1007/s00066-003-1150-9 (Pubitemid 37509467)
34. G.L. Wang B.H. Jiang E.A. Rue 1995 Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension Proc Natl Acad Sci USA 92 5510 4 7539918 10.1073/pnas.92.12.5510 1:CAS:528: DyaK2MXmtFOgs7k%3D
35. S. Welsh R. Williams L. Kirkpatrick 2004 Antitumor activity and pharmacodynamic properties of PX-478, an inhibitor of hypoxia-inducible factor-1alpha Mol Cancer Ther 3 233 44 15026543 1:CAS:528:DC%2BD2cXitFCnsrk%3D (Pubitemid 39193698)
36. M. Wolf F. Zehentmayr M. Niyazi 2010 Long-term outcome of mitomycin C- and 5-FU-based primary radiochemotherapy for esophageal cancer Strahlenther Onkol 186 374 81 20582393 10.1007/s00066-010-2137-y
37. K.J. Williams B.A. Telfer D. Xenaki 2005 Enhanced response to radiotherapy in tumours deficient in the function of hypoxia-inducible factor-1 Radiother Oncol 75 89 98 15878106 10.1016/j.radonc.2005.01.009 1:CAS:528:DC%2BD2MXjvVKmsrk%3D (Pubitemid 40616800)
38. K. Wurstbauer H. Weise H. Deutschmann 2010 Non-small cell lung cancer in stages I-IIIB: long-term results of definitive radiotherapy with doses. 80 Gy in standard fractionation Strahlenther Onkol 186 551 7 20936459 10.1007/s00066-010-2108-3
39. L. Yang W.K. Kang 2008 The effect of HIF-α siRNA on growth and chemosensitivity of Mia-paca cell line Yonsei Med J 49 295 300 18452268 10.3349/ymj.2008.49.2.295 1:CAS:528:DC%2BD1cXmvVSnur4%3D (Pubitemid 351781180)