Continuous CHO cell cultures with improved recombinant protein productivity by using mannose as carbon source: Metabolic analysis and scale-up simulation

Chemical Engineering Science

Volumn 66, Issue 11, 2011, Pages 2431-2439

  Berrios, Julio ^a   Altamirano, Claudia ^a   Osses, Nelson ^a   Gonzalez, Ramon ^b  

^a PONTIFICIA UNIVERSIDAD CATÓLICA DE VALPARAÍSO   (Chile)

^b Rice University   (United States)

Author keywords

Biochemical engineering; Biopharmaceuticals; Bioprocessing; Metabolism; Recombinant protein; Scale up

Indexed keywords

BIOMASS CONCENTRATIONS; BIOPHARMACEUTICALS; BIOPROCESSING; CARBON SOURCE; CELL CONCENTRATIONS; CELL METABOLISM; CHINESE HAMSTER OVARY CELLS; CHO CELL; CONSUMPTION RATES; CONTINUOUS CULTURE; CULTURE CONDITIONS; GLUCOSE CONTROL; METABOLIC ANALYSIS; OPERATING CONDITION; OXYGEN CONSUMPTION RATE; OXYGEN UPTAKE RATES; POTENTIAL APPLICATIONS; PRODUCTION PROCESS; PRODUCTIVE PROCESS; RECOMBINANT PROTEIN; SCALE-UP; SIALYLATION; SPECIFIC GROWTH RATE; SPECIFIC PRODUCTION RATE; STEADY-STATE OPERATION; TISSUE PLASMINOGEN ACTIVATOR; VOLUMETRIC PRODUCTIVITY;

ANIMAL CELL CULTURE; BIOCHEMICAL ENGINEERING; GLUCOSE; METABOLISM; OXYGEN; PHYSIOLOGY; PRODUCTIVITY; PROTEINS;

CELL CULTURE;

EID: 79953705943     PISSN: 00092509     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ces.2011.03.011     Document Type: Article

References (55)

1. Acevedo F. Transferencia de masa, momento y calor en fermentaciones. Fundamentos de Ingeniería Bioquímica, Ediciones Universitarias de Valparaíso, Valparaíso 2002, 217-251. F. Acevedo, J.C. Gentina, A. Illanes (Eds.).
2. Altamirano, C., 2000. Análisis del metabolismo de células cho recombinantes y desarrollo de estrategias de cultivo en medios definidos. Ph.D. Thesis, Universitat Autònoma de Barcelona, Spain.
3. Altamirano C., Cairó J., Gòdia F. Decoupling cell growth and product formation in Chinese hamster ovary cells through metabolic control. Biotechnology and Bioengineering 2001, 76:351-360.
4. Altamirano C., Illanes A., Becerra S., Cairó J., Gòdia F. Considerations on the lactate consumption by CHO cells in the presence of galactose. Journal of Biotechnology 2006, 125:547-556.
5. Altamirano C., Illanes A., Casablancas A., Gamez X., Cairó J., Gòdia F Analysis of CHO cells metabolic redistribution in a glutamate-based defined medium in continuous culture. Biotechnology Progress 2001, 17:1032-1041.
6. Altamirano C., Paredes C., Cairó J., Gòdia F. Improvement of CHO cell culture medium formulation: simultaneous substitution of glucose and glutamine. Biotechnology Progress 2000, 16:69-75.
7. Altamirano C., Paredes C., Illanes A., Cairó J.J., Gòdia F. Strategies for fed-batch cultivation of t-PA producing CHO cells: substitution of glucose and glutamine and rational design of culture medium. Journal of Biotechnology 2004, 110:171-179.
8. Berrios J., Díaz-Barrera A., Bazán C., Altamirano C. Relationship between tissue plasminogen activator production and specific growth rate in Chinese Hamster Ovary cells cultured in mannose at low temperature. Biotechnology Letters 2009, 31:1493-1497.
9. Blanch H., Clark D. Biochemical Engineering 1997, Marcel Dekker Inc., New York.
10. Bonarius H.P., de Gooijer C.D., Tramper J., Schmid G. Determination of the respiration quotient in mammalian cell culture in bicarbonate buffered media. Biotechnology and Bioengineering 1995, 45:524-535.
11. Bonarius H.P., Hatzimanikatis V., Meesters K.P., de Gooijer C.D., Schmid G., Tramper J. Metabolic flux analysis of hybridoma cells in different culture media using mass balances. Biotechnology and Bioengineering 1996, 50:299-318.
12. Çayli A., Hirschmann F., Wirth M., Hauser H., Wagner R. Cell lines with reduced UDP-N-acetylhexosamine pool in the presence of Ammonium. Biotechnology and Bioengineering 1999, 65:192-200.
13. Chen P., Harcum S. Effects of elevated ammonium on glycosylation gene expression in CHO cells. Metabolic Engineering 2006, 8:123-132.
14. Cinar A., Partulekar S., Ündey C. Batch Fermentation, Modelling, Monitoring and Control 2003, Marcel Dekker, New York.
15. Du P., Anumula K. Quantitative analysis of monosaccharides from glycoproteins by fast HPLC with highly sensitive fluorescence detection. Journal of Biomolecular Techniques 1998, 9:31-33.
16. Europa A., Gambhir A., Fu P, Hu W.-S. Multiple steady states with distinct cellular metabolism in continuous culture of mammalian cells. Biotechnology and Bioengineering 2000, 67:25-34.
17. Fenge C., Lüllau E. Cell culture bioreactors. Cell Culture Technology for Pharmaceutical and Cell-based 2006, 155-224. Taylor Francis, London. S.S. Ozkurt, W.-S. Hu (Eds.).
18. Ferrer-Miralles N., Domingo-Espín J., Corchero J.L., Vázquez E., Villaverde A. Microbial factories for recombinant pharmaceuticals. Microbial Cell Factories 2009, 8:17.
19. Follstad, B., 2004. Methods and media for controlling sialylation of proteins produced by mammalian cells. U.S. Patent No. 2004/00810.
20. 2 production rate of mammalian cell suspension culture based on off-gas measurement. Journal of Biotechnology 2002, 99:133-148.
21. Fu D., O'Neill R. Monosaccharide composition analysis of oligosaccharides and glycoproteins by high-perfomance liquid chromatography. Analytical Biochemistry 1995, 227:377-384.
22. Gambhir A., Korke R., Lee J., Fu P., Europa A., Hu W.-S. Analysis of cellular metabolism of hybridoma cells at distinct physiological states. Journal of Bioscience and Bioengineering 2003, 95:317-327.
23. Garcia-Ochoa F., Gomez E., Santos V.E., Merchuk J.C. Oxygen uptake rate in microbial processes: an overview. Biochemical Engineering Journal 2010, 49:289-307.
24. Gorenflo V.M., Smith L., Dedinsky B., Persson B., Piret J.M. Scale-up and optimization of an acoustic filter for 200L/day perfusion of a CHO cell culture. Biotechnology and Bioengineering 2002, 80:438-444.
25. Grillberger L., Kreil T.R., Nasr.S., Reiter M. Emerging trends in plasma-free manufacturing of recombinant protein therapeutics expressed in mammalian cells. Biotechnology Journal 2009, 4. 186-201.
26. Gu X., Wang D.I.C. Improvement of interferon-g sialylation in Chinese hamster ovary cell culture by feeding of N-acetylmannosamine. Biotechnology and Bioengineering 1998, 58:643-648.
27. Heussen C., Joubert F., Dowdle E.B. Purification of human tissue plasminogen activator with erythrina trypsin inhibitor. The Journal of Biological Chemistry 1984, 259:11635-11638.
28. Joziasse D., Lee R., Lee Y., Biessen E., Schiphorst W., Koeleman C., van den Eijnden D. α3-Galactosylated glycoproteins can bind to the hepatic asialoglycoprotein receptor. European Journal of Biochemistry 2000, 267:6501-6508.
29. Kompala D.S., Ozturk S.S Optimization of high cell density perfusion bioreactors. Cell Culture Technology for Pharmaceutical and Cell-based 2006, 387-416. Taylor Francis, London. S.S. Ozkurt, W.-S. Hu (Eds.).
30. Langheinrich C., Nienow A.W., Eddleston T., Stevenson N.C., Emery A.N., Clayton T.M., Slater N.K.H. Oxygen transfer in stirred bioreactors under animal cell culture conditions. Trans IChemE 2002, 80:39-44.
31. 3). African Journal of Biotechnology 2010, 9:7186-7191.
32. Ma N., Mollet M., Chalmers J.J. Aeration, mixing and hydrodynamics in bioreactors. Cell Culture Technology for Pharmaceutical and Cell-based 2006, Taylor Francis, London, pp. 225-248. S.S. Ozkurt, W.-S. Hu (Eds.).
33. Martinelle K., Doverskog.M., Jacobbson U., Chapman B., Kuchel P., Häggström L. Elevated glutamate dehydrogenase flux in glucose-deprived hybridoma and myeloma cells, evidence from 1H/15N NMR. Biotechnology and Bioengineering 1998, 60:508-517.
34. Matsunaga N., Kano K., Dobashi T. Culture scale-up studies as seen from the viewpoint of oxygen supply and dissolved carbon dioxide stripping. Journal of Bioscience and Bioengineering 2009, 107:412-418.
35. Najafpour G. Biochemical Engineering and Biotechnology 2007, Elsevier, Oxford.
36. Nelson D., Cox M. Lehninger Principles of Biochemistry 2004, W.H. Freeman, New York. fourth ed.
37. Nielsen, J., Villadsen, J., Lidén, G., 2003. Bioreaction Engineering Principles, second ed, Kluwer Academics, New York.
38. Nienow A. Reactor engineering in large scale animal cell culture. Cytotechnology 2006, 50:9-33.
39. Rodriguez P., Rivas C., Godoy A., Villanueva M., Fischbarg J., Vera J., Reyes M. Redefining the facilitated transport of mannose in human cells: absence of a glucose-insensitive, high-affinity facilitated mannose transport system. Biochemistry 2005, 44:313-320.
40. Ryll T., Valley U., Wagner R. Biochemistry of growth inhibition by ammonium ions in mammalian cells. Biotechnology and Bioengineering 1994, 44:184-193.
41. Selvarasu S., Kim D.Y., Karimi I.A., Lee D.-Y. Combined data preprocessing and multivariate statistical analysis characterizes fed-batch culture of mouse hybridoma cells for rational medium design. Journal of Biotechnology 2010, 150:94-100.
42. 2 influences on CHO cell growth, metabolism and IgG production. Biotechnology and Bioengineering 2007, 97:1479-1488.
43. Tisu M., Pavko A. Oxygen transfer in laboratory stirred tank bioreactor during mammalian cell culture cultivation. Acta Chimica Slovenica 2010, 57:123-128.
44. Tramper J., Smit D., Straatman J., Vlak J.M. Bubble column design for growth of fragile insect cells. Bioprocess Engineering 1988, 3:37-41.
45. Tsao Y., Cardoso A., Condon R., Voloch M., Lio P., Lagos J., Kearns B., Liu Z. Monitoring Chinese hamster ovary cell culture by the analysis of glucose and lactate metabolism. Journal of Biotechnology 2005, 118:290-303.
46. Varley J., Birch J. Reactor design for large scale suspension animal cell culture. Cytotechnology 1999, 29:177-205.
47. Wagner R. Metabolic control of animal cell culture processes. Mammalian Cell Biotechnology in Protein Production 1997, 233-276. Walter de Gruyter, Berlin. H. Hauser, R. Wagner (Eds.).
48. Weber W., Rimann M., de Glutza F.-N., Weber E., Memmert K., Fussenegger M. Gas-inducible product gene expression in bioreactors. Metabolic Engineering 2005, 7:174-181.
49. Wlaschin K., Hu W.-S. A scaffold for the Chinese hamster genome. Biotechnology and Bioengineering 2007, 98:429-439.
50. Xiao J., Shi Z., Gao P., Feng H., Duan Z., Mao Z. On-line optimization of glutamate production based on balanced metabolic control by RQ. Bioprocess and Biosystems Engineering 2006, 29:109-117.
51. Xie L., Zhou W. Fed-Batch cultivation of mammalian cells for the production of recombinant proteins. Cell culture Technology for Pharmaceutical and Cell-based 2006, 349-386. Taylor Francis, London. S.S. Ozkurt, W.-S. Hu (Eds.).
52. Xing Z., Kenty B.M., Li Z.J., Lee S.S. Scale-up analysis for a CHO cell culture process in large-scale bioreactors. Biotechnology and Bioengineering 2009, 103:733-746.
53. Xiong Z.-Q., Guo M.-J., Guo Y.-X., Zhuang Y.-P., Wang N.S., Zhang S.-Y. RQ feedback control for simultaneous improvement of GSH yield and GSH content in Saccharomyces cerevisiae T65. Enzyme and Microbial Technology 2010, 46:598-602.
54. Xiu Z.-L., Deckwer W.-D., Zeng A.-P. Estimation of rates of oxygen uptake and carbon dioxide evolution of animal cell culture using material and energy balances. Cytotechnology 1999, 29:159-166.
55. Zhang C., Shi Z., Gao P., Duan Z., Mao Z. On-line prediction of products concentrations in glutamate fermentation using metabolic network model and linear programming. Biochemical Engineering Journal 2005, 25:99-108.