Elevated glutamate dehydrogenase flux in glucose-deprived hybridoma and myeloma cells: Evidence from 1H/15N NMR

Biotechnology and Bioengineering

Volumn 60, Issue 4, 1998, Pages 508-517

  Martinelle, Kristina ^a   Doverskog, Magnus ^a   Jacobsson, Ulla ^a   Chapman, Bogdan E ^b   Kuchel, Philip W ^b   Häggström, Lena ^a  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^b UNIVERSITY OF SYDNEY   (Australia)

Author keywords

1H 15N NMR; Ammonium ions; Fructose; Glucose; Glutamate dehydrogenase; Glutaminase; Glutamine metabolism

Indexed keywords

ADENOSINETRIPHOSPHATE; AMINO ACIDS; AMMONIUM COMPOUNDS; CELLS; FRUCTOSE; GLUCOSE; METABOLISM; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

GLUTAMATE DEHYDROGENASE;

ENZYME KINETICS;

AMMONIA; FRUCTOSE; GLUCOSE; GLUTAMATE DEHYDROGENASE; GLUTAMINASE; GLUTAMINE;

AMINO ACID METABOLISM; ANIMAL CELL; ARTICLE; CELL METABOLISM; CONTROLLED STUDY; HYBRIDOMA; MOUSE; MYELOMA CELL; NONHUMAN; PROTON NUCLEAR MAGNETIC RESONANCE;

ANIMALS; BIOREACTORS; GLUCOSE; GLUTAMATE DEHYDROGENASE; HYBRIDOMAS; MAGNETIC RESONANCE SPECTROSCOPY; MICE; MODELS, BIOLOGICAL; MULTIPLE MYELOMA; TUMOR CELLS, CULTURED;

ANIMALIA;

EID: 0032553386     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19981120)60:4<508::AID-BIT13>3.0.CO;2-D     Document Type: Article

References (44)

1. Ardawi, M. S. M., Newsholme, E. A. 1983. Glutamine metabolism in lymphocytes of the rat. Biochem. J. 212: 835-842.
2. 15N-NMR study of ammonium assimilation in Agaricus bisporius. Biochem. Biophys. Acta 1310: 74-80.
3. Bidlingmeyer, B. A., Cohen, S. A., Tarvin, T. L. 1985. Rapid analysis of amino acids using precolumn derivatization. J. Chromatogr. 336: 93-104.
4. Bonarius, H. P. J., Hatzimanikatis, V., Meesters, K. P. H., Goijer, C. D. D., Schmid, G., Tramper, J. 1996. Metabolic flux analysis of hybridoma cells in different culture media using mass balances. Biotechnol. Bioeng. 50: 299-318.
5. Bray, H. G., James, S. P., Raffan, I. M., Thorpe, W. V. 1949. The enzymic hydrolysis of glutamine and its spontaneous decomposition in buffer solutions. Biochem. J. 44: 625-627.
6. 15N-labelled exchange. J. Magnetic Res. 56: 543-547.
7. Coleman, P. S., Lavietes, B. B. 1981. Membrane cholesterol, tumorigenesis, and the biochemical phenotype of neoplasia. CRC Crit. Rev. Biochem. 11: 341-393.
8. Del Giudice, R. A., Hopps, H. E. 1978. Microbiological methods and fluorescent microscopy for the direct demonstration of mycoplasma infection of cell cultures, pp. 57-69. In: G. J. McGarrity, D. G. Murphy, and W. W. Nichols (eds.), Mycoplasma infection of cell cultures. Plenum Press, New York.
9. 13N]glutamate in the Walker 256 carcinosarcoma in vivo. Cancer Res. 50: 4839-4844.
10. 15N NMR spectroscopy and imaging. J. Magnetic Res. Series B 102: 183-192.
11. Glacken, M. W. 1988. Catabolic control of mammalian cell culture. Bio/ Technol. 6: 1041-1050.
12. Harold, F. M. 1986. The vital force: a study of bioenergetics. W. H. Freeman and Co., New York.
13. Häggström, L. 1991. Energetics of glutaminolysis - a theoretical evaluation, pp. 79-81. In: R. E. Spier, J. B. Griffiths, and B. Meignier (eds.), Production of biologicals from animal cells in culture. Butterworth-Heinemann, Ltd., Oxford.
14. Jenkins, H. A., Butler, M., Dickson, A. J., 1992. Characterization of glutamine metabolism in two related murine hybridomas. J. Biotechnol. 23: 167-182.
15. Kurokawa, H., Park, Y. S., Iijima, S., Kobayashi, T. 1994. Growth characteristics in fed-batch culture of hybridoma cells with control of glucose and glutamine concentrations. Biotechnol. Bioeng. 44: 95-103.
16. 15N saturation transfer NMR spectroscopy. Am. J. Physiol. 268: C686-C699.
17. Lanks, K. W. 1986. Glutamine is responsible for stimulating glycolysis by L929 cells. J. Cell. Physiol. 126: 319-321.
18. Lide, D. R., ed. 1993-1994. Handbook of chemistry and physics. CRC Press, Inc., Boca Raton, FL.
19. Ljunggren, J., Häggström, L. 1992. Glutamine limited fed-batch culture reduces over-flow metabolism of amino acids in myeloma cells. Cytotechnol. 8: 45-56.
20. Ljunggren, J., Häggström, L. 1994. Catabolic control of hybridoma cells by glucose and glutamine limited fed batch cultures. Biotechnol. Bioeng. 44: 808-818.
21. Mancuso, A., Sharfstein, S. T., Tucker, S. N., Clark, D. S., Blanch, H. W. 1994. Examination of primary metabolic pathways in a murine hybridoma with carbon-13 nuclear magnetic resonance spectroscopy. Biotechnol. Bioeng. 44: 563-585.
22. Martinelle, K. 1997. On the energy metabolism and ammonium production in cultured animal cells. Ph.D. thesis, Royal Institute of Technology, Stockholm, Sweden.
23. Martinelle, K., Häggström, L. 1997. On the dissociation constant of ammonium: Effects of using an incorrect pKa in calculations of the ammonia concentration in animal cell cultures. Biotechnol. Techn. 11: 549-551.
24. + on the energy metabolism in murine myeloma cells. Cytotechnol., in press.
25. McKeehan, W. L. 1986. Glutaminolysis in animal cells, pp. 111-150. In: M. J. Morgan (ed.), Carbohydrate metabolism in cultured cells. Plenum Press, New York.
26. Meijer, J. J., van Dijken, J. P. 1995. Effects of glucose supply on myeloma growth and metabolism in chemostat cultures. J. Cell. Physiol. 162: 191-198.
27. Miller, W. M., Blanch, H. W., Wilke, C. R. 1988. A kinetic analysis of hybridoma growth and metabolism in batch and continuous suspension culture: Effect of nutrient concentration, dilution rate, and pH. Biotechnol. Bioeng. 32: 947-965.
28. Miller, W. M., Wilke, C. R., Blanch, H. W. 1989a. Transient responses of hybridoma cells to nutrient additions in continuous culture: I. Glucose pulse and step changes. Biotechnol. Bioeng. 33: 477-486.
29. Miller, W. M., Wilke, C. R., Blanch, H. W. 1989b. The transient responses of hybridoma cells to nutrient additions in continuous culture: II. Glutamine pulse and step changes. Biotechnol. Bioeng. 33: 487-499.
30. Moreadith, R. W., Lehninger, A. L. 1984. The pathways of glutamate and glutamine oxidation by tumor cell mitochondria. J. Biol. Chem. 259: 6215-6221.
31. Neermann, J., Wagner, R. 1996. Comparative analysis of glucose and glutamine metabolism in transformed mammalian cell lines, insect and primary liver cells. J. Cell. Physiol. 166: 152-169.
32. Oh, G.-S., Izuishi, T., Inoue, T., Hu, W.-S., Yoshida, T. 1996. Interactive dual control of glucose and glutamine feeding in hybridoma cultivation. J. Ferm. Bioeng. 81: 329-336.
33. Reitzer, L. J., Wice, B. M., Kennell, D. 1979. Evidence that glutamine, not sugar, is the major energy source for cultured HeLa cells. J. Biol. Chem. 254: 2669-2676.
34. Sharfstein, S. T., Tucker, S. N., Mancuso, A., Blanch, H. W., Clark, D. S. 1994. Quantitative in vivo nuclear magnetic resonance studies of hybridoma metabolism. Biotechnol. Bioeng. 43: 1059-1074.
35. Shulman, M., Wilde, C. D., Köhler, G. 1978. A better cell line for making hybridomas secreting specific antibodies. Nature 276: 269-270.
36. 31P-NMR studis of glucose and glutamine metabolism in cultured mammalian cells. Biochim. Biophys. Acta 1051: 131-137.
37. 15N N.M.R. study of nitrogen metabolism in cultured mammalian cells. Biochem. J. 291: 485-492.
38. Stryer, L. 1995. Biochemistry, 4th edition. W. H. Freeman and Co., New York.
39. Tipton, K. F., Couée, I. 1988. Glutamate dehydrogenase, pp. 81-100. In: E. Kvamme (ed.), Glutamine and glutamate in mammals, Vol. I, CRC Press, Inc., Boca Raton, FL.
40. Tritsch, G. L., Moore, G. E. 1962. Spontaneous decomposition of glutamine in cell culture media. Exp. Cell Res. 28: 360-364.
41. Vriezen, N., Romein, B., Luyben, K. C. A. M., d van Dijken, J. P. 1997. Effects of glutamine supply on growth and metabolism of mammalian cells in chemostat culture. Biotechnol. Bioeng. 54: 272-286.
42. Zeng, A.-P., Deckwer, W.-D. 1995. Mathematical modeling and analysis of glucose and glutamine utilization and regulation in cultures of continuous mammalian cells. Biotechnol. Bioeng. 47: 334-346.
43. Zielke, H. R., Ozand, P. T., Tildon, J. T., Sevdalian, D. A., Cornblath, M. 1976. Growth of human diploid fibroblasts in the absence of glucose utilization. Proc. Natl. Acad. Sci. USA 73: 4110-4114.
44. Zielke, H. R., Ozand, P. T., Tildon, J. T., Sevdalian, D. A., Cornblath, M. 1978. Reciprocal regulation of glucose and glutamine utilization by cultured human diploid fibroblasts. J. Cell. Physiol. 95: 41-48.