Estimation of rates of oxygen uptake and carbon dioxide evolution of animal cell culture using material and energy balances

Cytotechnology

Volumn 29, Issue 3, 1999, Pages 159-166

  Xiu, Zhi Long ^a   Deckwer, Wolf Dieter ^a   Zeng, An Ping ^a  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

Animal cell culture; Carbon dioxide evolution rate; Oxygen uptake rate; Reductance degree balance; Respiratory quotient

Indexed keywords

ALANINE; ANIMAL CELL CULTURE; BIOMASS; CARBON DIOXIDE; GLUTAMINE; HYBRIDOMA; MONOCLONAL ANTIBODY; OXYGEN;

EID: 0032891763     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008004618163     Document Type: Article

References (26)

1. Aunins JG and Henzler H-J (1993) Aeration in cell culture bioreactors. In Rehm H-J, Reed G, Pühler A and Stadler P (Eds) Biotechnology, Vol. 3, Bioprocessing, VCH, Weiheim.
2. Bonarius HPJ, de Gooijer CD, Tramper J and Schmid G (1995) Determination of the respiration quotient in mammalian cell culture in bicarbonate buffered media. Biotechnol Bioeng 45: 524-535.
3. Bonarius HPJ, Hatzimanikatis V, Meesters PH, de Gooijer CD, Schmid G and Tramper J (1996) Metabolic flux analysis of hybridoma cells in different culture media using mass balances. Biotechnol Bioeng 50: 299-318.
4. Edelman GM, Cunningham BA, Gall WE, Gottlieb PD, Rutishamer U and Waxdal MJ (1969) The covalent structure of an entire gamma G immunoglobulin molecule. Proc Nat Acad Sci USA 63: 78-85.
5. Erikson LE, Minkevich IG and Eroshin VK (1978) Application of mass and energy balance regularities in fermentation. Biotechnol Bioeng 20: 1595-1621.
6. Eyer K, Oeggerli A and Heinzle E (1995) On-line gas analysis in animal cell cultivation: II. Methods for oxygen uptake rate estimation and its application to controlled feeding of glutamine. Biotechnol Bioeng 45: 54-62.
7. Eyer K and Heinzle E (1996) On-line estimation of viable cells in a hybridoma culture at various DO levels using ATP balancing and redox potential measurement. Biotechnol Bioeng 49: 277-283.
8. Fitzpatrick L, Jenkins HA and Butler M (1993) Glucose and glutamine metabolism of a murine B-lymphocyte hybridoma grown in batch culture. Appl Biochem Biotechnol 43: 93-116.
9. Frame KK and Hu W-S (1990) Cell volume measurement as an estimation of mammalian cell biomass. Biotechnol Bioeng 36: 191-197.
10. 2 in large-scale and high-density CHO cell perfusion culture. Cytotechnol 22: 65-78.
11. Hiller GW, Aeschlimann AD, Clark DS and Blanch HW (1991) A kinetic analysis of hybridoma growth and metabolism in continuous suspension culture on serum-free medium. Biotechnol Bioeng 38: 733-741.
12. Hiller GW, Clark DS and Blanch HW (1993) Cell retention - chemostat studies of hybridoma cells - analysis of hybridoma growth and metabolism in continuous suspension culture on serum-free medium. Biotechnol Bioeng 42: 185-195.
13. Jan DCH, Petch DA, Huzel N and Butler M (1997) The effect of dissolved oxygen on the metabolic profile of a murine hybridoma grown in serum-free medium in continuous culture. Biotechnol Bioeng 54: 153-164.
14. Lovrecz G and Gray P (1994) Use of on-line gas analysis to monitor recombinant mammalian cell cultures. Cytotechnol 14: 167-175.
15. Martens DE, Sipkema EM, de Gooijer CD, Beuvery EC and Tramper J (1995) A combined cell-cycle and metabolic model for the growth of hybridoma cells in steady-state continuous culture. Biotechnol Bioeng 48: 49-65.
16. Savinell JM and Palsson BO (1992) Network analysis of intermediary metabolism using linear optimization. I Development of mathematical formalism. J Theor Biol 154: 421-454.
17. Wang HY, Cooney CL and Wang DIC (1977) Computer-aided baker's yeast fermentation. Biotechnol Bioeng 19: 69-86.
18. Xie L and Wang DIC (1994) Applications of improved stoichiometric model in medium design and fed-batch cultivation of animal cells in bioreactor. Cytotechnol 15: 17-29.
19. Xie L and Wang DIC (1996) Material balance studies on animal cell metabolism using a stoichiometrically based reaction network. Biotechnol Bioeng, 52: 579-590.
20. Yoon S-J and Konstantinov K (1994) Continuous real-time monitoring of oxygen uptake rate (OUR) in animal cell bioreactors. Biotechnol Bioeng 44: 170-177.
21. Zeng A-P, Byun T-G, Posten C and Deckwer W-D (1994) Use of respiratory quotient as a control parameter for optimum oxygen supply and scale-up of 2,3-butanediol production under microaerobic conditions. Biotechnol Bioeng 44: 1107-1114.
22. 2 evolution rate in aerobic and anaerobic continuous cultures. Appl Microbiol Biotechnol 42: 688-691.
23. Zeng A-P (1995) A new balance equation of reducing equivalents for data consistency check and bioprocess calculation. J Biotechnol 43: 111-124.
24. Zhou W, Rehm J and Hu W-S (1995) High viable cell concentration fed-batch cultures of hybridoma cells through on-line nutrient feeding. Biotechnol Bioeng 46: 579-587.
25. 13C NMR. Biotechnol Bioeng 45: 292-303.
26. Zupke C, Sinskey AJ and Stephanopoulos G (1995b) Intracellular flux analysis applied to the effect of dissolved oxygen on hybridomas. Appl Microbiol Biotechnol 44: 27-36.