Relationship between tissue plasminogen activator production and specific growth rate in Chinese Hamster Ovary cells cultured in mannose at low temperature

Biotechnology Letters

Volumn 31, Issue 10, 2009, Pages 1493-1497

  Berrios, Julio ^a   Díaz Barrera, Alvaro ^a   Bazán, Consuelo ^a   Altamirano, Claudia ^a  

^a PONTIFICIA UNIVERSIDAD CATÓLICA DE VALPARAÍSO   (Chile)

Author keywords

CHO cells; Glutamate; Low temperature; Mannose; Specific growth rate; tPA

Indexed keywords

CHO CELLS; GLUTAMATE; LOW TEMPERATURE; MANNOSE; SPECIFIC GROWTH RATE; TPA;

CELL MEMBRANES; GLUCOSE; GROWTH RATE;

LOW TEMPERATURE PRODUCTION;

MANNOSE; RECOMBINANT PROTEIN; TISSUE PLASMINOGEN ACTIVATOR;

ANIMAL; ARTICLE; BIOSYNTHESIS; CELL PROLIFERATION; CHEMISTRY; CHO CELL; CRICETULUS; CULTURE MEDIUM; FEMALE; GENETICS; HAMSTER; HUMAN; METABOLISM; STATISTICS; TEMPERATURE;

ANIMALS; CELL PROLIFERATION; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA; FEMALE; HUMANS; MANNOSE; RECOMBINANT PROTEINS; STATISTICS AS TOPIC; TEMPERATURE; TISSUE PLASMINOGEN ACTIVATOR;

CRICETULUS GRISEUS;

EID: 70350103519     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-009-0050-1     Document Type: Article

References (19)

1. Al-Fageeh M, Marchant R, Carden M, Smales CM (2006) The cold-shock response in cultured mammalian cells: harnessing the response for the improvement of recombinant protein production. Biotechnol Bioeng 93:829-835.
2. Al-Rubeai M, Singh RP (1998) Apoptosis in cell culture. Curr Opin Biotechnol 9:152-156.
3. Altamirano C, Illanes A, Becerra S, Cairó J, Gòdia F (2006) Considerations on the lactate consumption by CHO cells in the presence of galactose. J Biotechnol 125:547-556.
4. Berrios J, Altamirano C (2009) Mannose as carbon source improves productivity and sialic acid content of tPA produced by CHO cells in continuous cultures. Submitted to J Biotechnol.
5. Bollati-Fogolin M, Wagner R, Etcheverrigaray M, Kratje R (2004) Impact of temperature reduction and expression of yeast pyruvate carboxylase on hGM-CSF-producing CHO cells. J Biotechnol 109:179-191.
6. Bollati-Fogolin M, Forno G, Nimtz M, Conradt HS, Etcheverrigaray M, Kratje R (2005) Temperature reduction in cultures of hGM-CSF-expressing CHO cells: effect on productivity and product quality. Biotechnol Prog 21:17-21.
7. Butler M (2005) Animal cell cultures: recent achievements and perspectives in the production of biopharmaceuticals. Appl Microbiol Biotechnol 68:283-291.
8. Du P, Anumula K (1998) Quantitative analysis of monosaccharides from glycoproteins by fast HPLC with highly sensitive fluorescence detection. J Biomol Tech 9:31-33.
9. Fox SR, Tan HK, Tan MC, Wong SC, Yap MG, Wang DI (2005) A detailed understanding of the enhanced hypothermic productivity of interferon-gamma by Chinese-Hamster Ovary cells. Biotechnol Appl Biochem 41:255-264.
10. Fussenegger M, Schlatter S, Datwyler D, Mazur X, Bailey JE (1998) Controlled proliferation by multigene metabolic engineering enhances the productivity of Chinese Hamster Ovary cells. Nat Biotechnol 16:468-472.
11. Hendrick V, Winnepenninckx P, Abdelkafi CV, Andeputte O, Cherlet M, Marique T, Renemann G, Loa A, Kretzmer G, Werenne J (2001) Increased productivity of recombinant tissular plasminogen activator (t-PA) by butyrate and shift of temperature: a cell cycle phases analysis. Cytotechnology 36:71-83.
12. Lipscomb M, Palomares L, Hernández V, Ramírez O, Kompala D (2005) Effect of production method and gene amplification on the glycosylation pattern of a secreted reporter protein in CHO cells. Biotechnol Prog 21:40-49.
13. Ngo TT, Phan APH, Yam CF, Lenhoff HM (1982) Interference in determination of ammonia with the hypochlorite-alkaline phenol method of Berthelot. Anal Chem 54:46-49.
14. Renard J, Spagnoli R, Maizer C, Salles MF, Mandine E (1988) Evidence that monoclonal antibody production kinetics is related to the integral of viable cells curve in batch systems. Biotechnol Lett 10:91-96.
15. Takuma S, Hirashima C, Piret J (2007) Dependence on glucose limitation of the pCO2 influences on CHO cell growth, metabolism and IgG production. Biotechnol Bioeng 97:1479-1488.
16. Wagner R (1997) Metabolic control of animal cell culture processes. In: Hauser H, Wagner R (eds) Mammalian cell biotechnology in protein production. Walter de Gruyter, Berlin, pp 233-276.
17. Wong DC, Wong KT, Lee YY, Morin PN, Heng CK, Yap MG (2006) Transcriptional profiling of apoptotic pathways in batch and fed-batch CHO cell cultures. Biotechnol Bioeng 94:373-382.
18. Yokota M, Tanji Y (2008) Analysis of cell-cycle-dependent production of tissue plasminogen activator analogue (pamiteplase) by CHO cells. Biochem Eng J 39:297-304.
19. Yoon S, Song J, Lee G (2003) Effect of low culture temperature on specific productivity, transcription level, and heterogeneity of erythropoietin in Chinese Hamster Ovary cells. Biotechnol Bioeng 82:289-298.