A practical method for cell-free protein synthesis to avoid stable isotope scrambling and dilution

Analytical Biochemistry

Volumn 411, Issue 2, 2011, Pages 223-229

  Yokoyama, Jun ^a,b,c   Matsuda, Takayoshi ^a   Koshiba, Seizo ^a,d   Tochio, Naoya ^a   Kigawa, Takanori ^a,b  

^a RIKEN   (Japan)

^b TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^c Taiyo Nippon Sanso   (Japan)

^d YOKOHAMA CITY UNIVERSITY   (Japan)

Author keywords

Amino acid selective labeling; Cell free protein synthesis; Deuterium labeling; NMR; Stable isotope labeling

Indexed keywords

AMINO ACIDS; CELLS; CYTOLOGY; ESCHERICHIA COLI; ISOTOPES; NUCLEAR MAGNETIC RESONANCE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEINS;

CELL FREE PROTEIN SYNTHESIS; CONVENTIONAL SYSTEMS; DEUTERIUM LABELING; ESCHERICHIA COLI CELLS; METABOLIC REACTIONS; PROTEIN STRUCTURE ANALYSIS; SELECTIVE LABELING; STABLE-ISOTOPE LABELING;

BIOSYNTHESIS;

5 DIAZO 4 OXO LEVO NORVALINE; 6 DIAZO 5 OXONORLEUCINE; AMINOOXYACETIC ACID; ASPARAGINE; ASPARTIC ACID; GLUTAMIC ACID; GLUTAMINE; ISOTOPE; MALIC ACID; METHIONINE SULFOXIMINE; NORVALINE; S METHYL LEVO CYSTEINE SULFOXIMINE; SULFUR AMINO ACID; UBIQUITIN; UNCLASSIFIED DRUG;

AMINO ACID METABOLISM; AMINO ACID SEQUENCE; ARTICLE; BACTERIAL CELL; CARBON NUCLEAR MAGNETIC RESONANCE; CELL FREE SYSTEM; CHEMICAL LABELING; ESCHERICHIA COLI; ISOTOPE DILUTION ASSAY; PRIORITY JOURNAL; PROTEIN SYNTHESIS; PROTON NUCLEAR MAGNETIC RESONANCE; PROTON TRANSPORT;

ESCHERICHIA COLI;

EID: 79952452377     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.01.017     Document Type: Article

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