An Escherichia coli-based cell-free system for large-scale production of functional mammalian membrane proteins suitable for X-ray crystallography

Journal of Molecular Microbiology and Biotechnology

Volumn 18, Issue 2, 2010, Pages 85-91

  Nguyen, Tuan A ^a   Lieu, Samantha S ^a   Chang, Geoffrey ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Cell free expression; Escherichia coli; Membrane protein; X ray structural analysis

Indexed keywords

GAP JUNCTION PROTEIN; MEMBRANE PROTEIN; OLIGOMER;

ARTICLE; CELL FREE SYSTEM; CONTROLLED STUDY; CRYSTALLIZATION; ESCHERICHIA COLI; EXTRACTION; GENE EXPRESSION SYSTEM; NONHUMAN; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; X RAY CRYSTALLOGRAPHY;

BIOTECHNOLOGY; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; HUMANS; MEMBRANE PROTEINS; PROTEIN FOLDING;

ESCHERICHIA COLI; MAMMALIA;

EID: 76549098201     PISSN: 14641801     EISSN: None     Source Type: Journal    
DOI: 10.1159/000283512     Document Type: Article

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