Efficient condensed-phase production of perdeuterated soluble and membrane proteins

Journal of Structural and Functional Genomics

Volumn 11, Issue 2, 2010, Pages 143-154

  Schneider, William M ^a   Tang, Yuefeng ^a   Vaiphei, S Thangminlal ^a,b   Mao, Lili ^a,b   Maglaqui, Melissa ^b   Inouye, Masayori ^a   Roth, Monica J ^a   Montelione, Gaetano T ^a,b  

^a RUTGERS ROBERT WOOD JOHNSON MEDICAL SCHOOL   (United States)

^b RUTGERS UNIVERSITY   (United States)

Author keywords

Perdeuteration; Protein expression in E. coli; Protein NMR; Single protein production system (SPP); Triple resonance NMR

Indexed keywords

COLD SHOCK PROTEIN; COLD SHOCK PROTEIN A; ESCHERICHIA COLI PROTEIN; MEMBRANE PROTEIN; MEMBRANE PROTEIN YAIZ; OUTER MEMBRANE PROTEIN; OUTER MEMBRANE PROTEIN X; PROTEIN ENVZB; UNCLASSIFIED DRUG; CSPA PROTEIN, E COLI; DEUTERIUM; ENVZ PROTEIN, E COLI; HEAT SHOCK PROTEIN; MULTIENZYME COMPLEX;

ARTICLE; BACTERIUM CULTURE; BIOTECHNOLOGICAL PROCEDURES; BIOTECHNOLOGICAL PRODUCTION; CARBON NUCLEAR MAGNETIC RESONANCE; CONDENSED SINGLE PROTEIN PRODUCTION METHOD; CONTROLLED STUDY; CRYSTALLOGRAPHY; DEUTERON NUCLEAR MAGNETIC RESONANCE; ESCHERICHIA COLI; FERMENTATION MEDIUM; NITROGEN NUCLEAR MAGNETIC RESONANCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BLOOD LEVEL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN POLYMERIZATION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; CHEMISTRY; GENE EXPRESSION; GENE VECTOR; GENETICS; MASS SPECTROMETRY; METABOLISM; NUCLEAR MAGNETIC RESONANCE;

BACTERIAL OUTER MEMBRANE PROTEINS; DEUTERIUM; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; GENETIC VECTORS; HEAT-SHOCK PROTEINS; MEMBRANE PROTEINS; MULTIENZYME COMPLEXES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; COLD SHOCK PROTEINS AND PEPTIDES;

EID: 77956749496     PISSN: 1345711X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10969-010-9083-x     Document Type: Article

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