Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy

European Journal of Biochemistry

Volumn 271, Issue 20, 2004, Pages 4084-4093

  Ozawa, Kiyoshi ^a   Headlam, Madeleine J ^a   Schaeffer, Patrick M ^a   Henderson, Blair R ^a   Dixon, Nicholas E ^a   Otting, Gottfried ^a  

^a AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

Author keywords

15N HSQC spectrum; Cell free protein synthesis; Human cyclophilin A; NMR; Selective stable isotope labeling

Indexed keywords

ACETIC ACID; AMINO ACID; AMMONIA; ASPARAGINE; ASPARTIC ACID; CYCLOPHILIN A; NITROGEN 15; RNA POLYMERASE; SYNTHETIC DNA;

ARTICLE; BACTERIOPHAGE LAMBDA; BACTERIOPHAGE T7; CHROMATOGRAPHY; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC TRANSCRIPTION; HUMAN; ISOTOPE LABELING; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; TRANSAMINATION; ULTRACENTRIFUGATION;

AMINO ACIDS; AMINO ACYL-TRNA SYNTHETASES; ANIMALS; BACILLUS SUBTILIS; BACTERIOPHAGE LAMBDA; BACTERIOPHAGE T7; CATTLE; CELL-FREE SYSTEM; CYCLOPHILIN A; CYCLOPHILINS; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HUMANS; ISOTOPE LABELING; KINETICS; LUPINUS; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PARACOCCUS DENITRIFICANS; PEPTIDYLPROLYL ISOMERASE; PROMOTER REGIONS (GENETICS); PROTEIN BIOSYNTHESIS; SACCHAROMYCES; VIRAL PROTEINS;

BACTERIOPHAGE LAMBDA; ESCHERICHIA COLI; NEGIBACTERIA; SYNTHETIC CONSTRUCT;

EID: 7044263294     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.2004.04346.x     Document Type: Article

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