A robust two-step PCR method of template DNA production for high-throughput cell-free protein synthesis

Journal of Structural and Functional Genomics

Volumn 8, Issue 4, 2007, Pages 173-191

  Yabuki, Takashi ^a   Motoda, Yoko ^a   Hanada, Kazuharu ^a   Nunokawa, Emi ^a   Saito, Miyuki ^a   Seki, Eiko ^a   Inoue, Makoto ^a   Kigawa, Takanori ^a,b   Yokoyama, Shigeyuki ^a,c  

^a RIKEN YOKOHAMA INSTITUTE   (Japan)

^b TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^c UNIVERSITY OF TOKYO   (Japan)

Author keywords

Cell free protein synthesis; Human proteins; Linear template construction; Tagged proteins; Two step PCR

Indexed keywords

COMPLEMENTARY DNA; DNA; PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CELL FREE SYSTEM; DNA SYNTHESIS; DNA TEMPLATE; ESCHERICHIA COLI; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN SYNTHESIS; RIBOSOME; TRANSCRIPTION REGULATION; TRANSLATION REGULATION; UNINDEXED SEQUENCE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CELL-FREE SYSTEM; CELLS, CULTURED; DNA; DNA PRIMERS; ESCHERICHIA COLI; HUMANS; MOLECULAR SEQUENCE DATA; PLASMIDS; POLYMERASE CHAIN REACTION; PROTEIN BIOSYNTHESIS; SEQUENCE HOMOLOGY, NUCLEIC ACID;

ESCHERICHIA COLI;

EID: 41049089651     PISSN: 1345711X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10969-007-9038-z     Document Type: Article

References (28)

1. Nameki N, Tochio N, Koshiba S, Inoue M, Yabuki T, Aoki M, Seki E, Matsuda T, Fujikura Y, Saito M, Ikari M, Watanabe M, Terada T, Shirouzu M, Yoshida M, Hirota H, Tanaka A, Hayashizaki Y, Guntert P, Kigawa T, Yokoyama S (2005) Protein Sci 14:756-764
2. Yamasaki K, Kigawa T, Inoue M, Tateno M, Yamasaki T, Yabuki T, Aoki M, Seki E, Matsuda T, Tomo Y, Hayami N, Terada T, Shirouzu M, Tanaka A, Seki M, Shinozaki K, Yokoyama S (2005) Plant Cell 17:944-956
3. Li H, Inoue M, Yabuki T, Aoki M, Seki E, Matsuda T, Nunokawa E, Motoda Y, Kobayashi A, Terada T, Shirouzu M, Koshiba S, Lin YJ, Guntert P, Suzuki H, Hayashizaki Y, Kigawa T, Yokoyama S (2005) J Biomol NMR 32:329-334
4. Yabuki T, Kigawa T, Dohmae N, Takio K, Terada T, Ito Y, Laue ED, Cooper JA, Kainosho M, Yokoyama S (1998) J Biomol NMR 11:295-306
5. Kurimoto K, Muto Y, Obayashi N, Terada T, Shirouzu M, Yabuki T, Aoki M, Seki E, Matsuda T, Kigawa T, Okumura H, Tanaka A, Shibata N, Kashikawa M, Agata K, Yokoyama S (2005) J Struct Biol 150:58-68
6. Arai R, Kukimoto-Niino M, Uda-Tochio H, Morita S, Uchikubo-Kamo T, Akasaka R, Etou Y, Hayashizaki Y, Kigawa T, Terada T, Shirouzu M, Yokoyama S (2005) Protein Sci 14:1888-1893
7. Kigawa T, Yamaguchi-Nunokawa E, Kodama K, Matsuda T, Yabuki T, Matsuda N, Ishitani R, Nureki O, Yokoyama S (2002) J Struct Funct Genomics 2:29-35
8. Kang SH, Kim DM, Kim HJ, Jun SY, Lee KY, Kim HJ (2005) Biotechnol Prog 21:1412-1419
9. Ryabova LA, Desplancq D, Spirin AS, Pluckthun A (1997) Nat Biotechnol 15:79-84
10. Klammt C, Schwarz D, Lohr F, Schneider B, Dotsch V, Bernhard F (2006) FEBS J 273:4141-4153
11. Yokoyama S, Matsuo Y, Hirota H, Kigawa T, Shirouzu M, Kuroda Y, Kurumizaka H, Kawaguchi S, Ito Y, Shibata T, Kainosho M, Nishimura Y, Inoue Y, Kuramitsu S (2000) Prog Biophys Mol Biol 73:363-376
12. Endo Y, Sawasaki T (2004) J Struct Funct Genomics 5:45-57
13. Sawasaki T, Ogasawara T, Morishita R, Endo Y (2002) Proc Natl Acad Sci USA 99:14652-14657
14. Woodrow KA, Airen IO, Swartz JR (2006) J Proteome Res 5:3288-3300
15. Kigawa T, Muto Y, Yokoyama S (1995) J Biomol NMR 6:129-134
16. Nakai K, Horton P (1999) Trends Biochem Sci 24:34-36
17. Dougherty WG, Cary SM, Parks TD (1989) Virology 171:356-364
18. Chaga G, Bochkariov DE, Jokhadze GG, Hopp J, Nelson P (1999) J Chromatogr A 864:247-256
19. Hirota Y, Katsumata A, Takeya T (1990) Nucleic Acids Res 18:6432
20. Keefe AD, Wilson DS, Seelig B, Szostak JW (2001) Protein Expr Purif 23:440-446
21. Matsuda T, Kigawa T, Koshiba S, Inoue M, Aoki M, Yamasaki K, Seki M, Shinozaki K, Yokoyama S (2006) J Struct Funct Genomics 7:93-100
22. Lin-Goerke JL, Robbins DJ, Burczak JD (1997) Biotechniques 23:409-412
23. Kigawa T, Yabuki T, Yoshida Y, Tsutsui M, Ito Y, Shibata T, Yokoyama S (1999) FEBS Lett 442:15-19
24. Nakano H, Kobayashi K, Ohuchi S, Sekiguchi S, Yamane T (2000) J Biosci Bioeng 90:456-458
25. Esposito D, Chatterjee DK (2006) Curr Opin Biotechnol 17:353-358
26. Labaer J, Ramachandran N (2005) Curr Opin Chem Biol 9:14-19
27. Griffiths AD, Tawfik DS (2006) Trends Biotechnol 24:395-402
28. Waldo GS, Standish BM, Berendzen J, Terwilliger TC (1999) Nat Biotechnol 17:691-695