An economical method for producing stable-isotope labeled proteins by the E. coli cell-free system

Journal of Biomolecular NMR

Volumn 48, Issue 4, 2010, Pages 193-201

  Yokoyama, Jun ^a,b,c   Matsuda, Takayoshi ^a   Koshiba, Seizo ^a,d   Kigawa, Takanori ^a,b  

^a RIKEN   (Japan)

^b TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^c TAIYO NIPPON SANSO CORPORATION   (Japan)

^d YOKOHAMA CITY UNIVERSITY   (Japan)

Author keywords

Cell free protein synthesis; In vitro translation; Metabolic conversion; Stable isotope labeling

Indexed keywords

AMINO ACID; ASPARAGINE; CELL EXTRACT; CYSTEINE; GLUTAMINE; INDOLE; SODIUM SULFIDE; STABLE ISOTOPE; TRYPTOPHAN;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL CELL; CELL FREE SYSTEM; CHEMICAL LABELING; COST BENEFIT ANALYSIS; COST CONTROL; ESCHERICHIA COLI; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN SYNTHESIS;

AMINO ACIDS; ASPARAGINE; CYSTEINE; ESCHERICHIA COLI; GLUTAMINE; ISOTOPE LABELING; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN BIOSYNTHESIS; PROTEINS; TRYPTOPHAN;

ALGAE; ESCHERICHIA COLI;

EID: 78651309787     PISSN: 09252738     EISSN: 15735001     Source Type: Journal    
DOI: 10.1007/s10858-010-9455-3     Document Type: Article

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