Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library

Nature Biotechnology

Volumn 24, Issue 12, 2006, Pages 1569-1571

  Mena, Marco A ^a   Treynor, Thomas P ^c   Mayo, Stephen L ^b,c   Daugherty, Patrick S ^a,b  

^a Santa Barbara   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c California Institute of Technology HHMI   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; BIODIVERSITY; CELLS; CHROMOPHORES; FLUORESCENCE; PH EFFECTS; QUANTUM THEORY; SENSITIVITY ANALYSIS;

BLUE FLUORESCENT PROTEIN (BFP); MAMMALIAN CELLS; PHOTOBLEACHING; PHOTOSTABILITY;

PROTEINS;

BLUE FLUORESCENT PROTEIN; PROTEIN;

ARTICLE; BACTERIAL CELL; BLEACHING; CELL ASSAY; CELL CLONE; CHROMATOPHORE; CYTOMETRY; FLOW CYTOMETRY; FLUORESCENCE; FLUOROMETRY; GENE LIBRARY; GENE STRUCTURE; IMAGING; MAMMAL CELL; NUCLEOTIDE SEQUENCE; PH; PRIORITY JOURNAL; PROTEIN STABILITY; QUANTUM YIELD; SCREENING;

FLOW CYTOMETRY; GENE LIBRARY; LUMINESCENT MEASUREMENTS; LUMINESCENT PROTEINS; PHOTOBLEACHING; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); MAMMALIA;

EID: 33845690052     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt1264     Document Type: Article

References (19)

1. Tsien, R.Y. Annu. Rev. Biochem. 67, 509-544 (1998).
2. Cubitt, A.B. et al. Trends Biochem. Sci. 20, 448-455 (1995).
3. Shaner, N.C., Steinbach, P.A. & Tsien, R.Y. Nat. Methods 2, 905-909 (2005).
4. Heim, R. & Tsien, R.Y. Curr. Biol. 6, 178-182 (1996).
5. Stauber, R.H. et al. Biotechniques 24, 462-466, 468-471 (1998).
6. Ellenberg, J., Lippincott-Schwartz, J. & Presley, J.F. Trends Cell Biol. 9, 52-56 (1999).
7. Lippincott-Schwartz, J. & Patterson, G.H. Science 300, 87-91 (2003).
8. Park, S., Yang, X. & Saven, J.G. Curr. Opin. Struct. Biol. 14, 487-494 (2004).
9. Dahiyat, B.I. & Mayo, S.L. Science 278, 82-87 (1997).
10. Neylon, C. Nucleic Acids Res. 32, 1448-1459 (2004).
11. Voigt, C.A., Mayo, S.L., Arnold, F.H. & Wang, Z.G. Proc. Natl. Acad. Sci. USA 98, 3778-3783 (2001).
12. Hayes, R.J. et al. Proc. Natl. Acad. Sci. USA 99, 15926-15931 (2002).
13. Wachter, R.M. et al. Biochemistry 36, 9759-9765 (1997).
14. Treynor, T.P., Vizcarra, C.L., Nedelcu, D. & Mayo, S.L. Proc. Natl. Acad. Sci. USA (in the press).
15. Bessette, P.H., Mena, M.A., Nguyen, A.W. & Daugherty, P.S. Methods Mol. Biol. 231, 29-37 (2003).
16. Campbell, R.E. et al. Proc. Natl. Acad. Sci. USA 99, 7877-7882 (2002).
17. Ellenberg, J., Lippincott-Schwartz, J. & Presley, J.F. Biotechniques 25, 838-842, 844-836 (1998).
18. Kummer, A.D. et al. Chembiochem 3, 659-663 (2002).
19. Mauring, K. et al. J. Phys. Chem. B 109, 12976-12981 (2005).