Production of a biotinylated single-chain antibody fragment in the cytoplasm of Escherichia coli

Journal of Immunological Methods

Volumn 284, Issue 1-2, 2004, Pages 165-175

  Santala, Ville ^a   Lamminmäki, Urpo ^a  

^a UNIVERSITY OF TURKU   (Finland)

Author keywords

Immunoassay; In vivo biotinylation; Oxidizing bacterial cytoplasm; scFv; Single chain antibody fragment expression

Indexed keywords

BIOTIN; CARRIER PROTEIN; HYBRID PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; THYROID STIMULATING IMMUNOGLOBULIN;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL STRAIN; BIOTINYLATION; CELL DISRUPTION; CYTOPLASM; ESCHERICHIA COLI; IMMUNOASSAY; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; TEMPERATURE DEPENDENCE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0347634531     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2003.10.008     Document Type: Article

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