Eicosapentaenoic acid (EPA) biosynthetic gene cluster of Shewanella oneidensis MR-1: Cloning, heterologous expression, and effects of temperature and glucose on the production of EPA in Escherichia coli

Biotechnology and Bioprocess Engineering

Volumn 11, Issue 6, 2006, Pages 510-515

  Lee, Su Jin ^a   Jeong, Young Su ^a   Kim, Dong Uk ^a   Seo, Jeong Woo ^b   Hur, Byung Ki ^a  

^a Inha University   (South Korea)

^b Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

Eicosapentaenoic acid; EPA biosynthetic gene cluster; Polyketide synthase like pathway; Polyunsaturated fatty acids; Shewanella oneidensis MR 1

Indexed keywords

ACYLTRANSFERASE; BETA KETOACYL SYNTHASE; DNA FRAGMENT; ENOYL REDUCTASE; GLUCOSE; ICOSAPENTAENOIC ACID; PHOSPHOPENTETHEINE TRANSFERASE; POLYKETIDE SYNTHASE; REGULATOR PROTEIN; RESTRICTION ENDONUCLEASE; SYNTHETASE; TRANSFERASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BACTERIUM CULTURE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; COSMID VECTOR; ENVIRONMENTAL FACTOR; ENZYME ACTIVITY; ESCHERICHIA COLI; FATTY ACID SYNTHESIS; GENE AMPLIFICATION; GENE CLUSTER; GENE EXPRESSION REGULATION; GENE MAPPING; GENE SEQUENCE; HETEROLOGOUS EXPRESSION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PROTEIN STABILITY; REGULATORY MECHANISM; SHEWANELLA ONEIDENSIS; TEMPERATURE;

ESCHERICHIA COLI; SHEWANELLA ONEIDENSIS MR-1;

EID: 33846072518     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02932075     Document Type: Article

References (23)

1. Lauritzen, L., H. S. Hansen, M. H. Jorgensen, and K. F. Michaelsen (2001) The essentiality of long chain n-3 fatty acids in relation to development and function of the brain and retina. Prog. Lipid Res. 40: 1-94.
2. Braden, L. M. and K. K. Caroll (1986) Dietary polyunsaurated fat in relation to mammary carcinogenesis in rats. Lipids 21: 285-288.
3. Abbey, M., P. Clifton, M. Kestin, B. Belling, and P. Nestel (1990) Effect of fish oil on lipoproteins, lecithin: cholesterol acyltransferase, and lipid transfer protein activity in humans. Arteriosclerosis 10: 85-94.
4. Sauer, L. A., R. T. Dauchy, and D. E. Blask (2001) Polyunsaturated fatty acids, melatonin, and cancer prevention. Biochem. Pharmacol. 61: 1455-1462.
5. Angerer, P. and C. von Schacky (2000) n-3 polyunsaturated fatty acids and the cardiovascular system. Curr. Opin. Clin. Nutr. Metab. Care 3: 439-445.
6. Tresguerres, J. A. F., V. Lahera, and M. V. Cachofeiro (1989) Dietary essential polyunsaturated fatty acids and blood pressure News Physiol. Sci. 4: 64-68.
7. Fiocchi, A., M. Sala, P. Signoroni, G. Banderal, C. Agostoul, and E. Riva (1994) The efficiency and safety of γ-linolenic acid in the treatment of infantile atopic dermatitis. J. Int. Med. Res. 22: 2-32.
8. Chung, T.-H., S.-J. Lee, H.-J. Oh, G.-J. Kim, and B.-K. Hur (2005) Cloning of a Δ5 desaturase from Thraustochytrium sp. 26185 and functional expression in Pichia pastoris. Korean J. Biotechnol. Bioeng. 20: 93-99.
9. Wallis, J. G., J. L. Watts, and J. Browse (2002) Polyunsaturated fatty acid synthesis: what will they think of next? Trends Biochem. Sci. 27: 467-473.
10. Qiu, X. (2003) Biosynthesis of docosahexaenoic acid (DHA, 22:6-4, 7,10,13,16,19): two distinct pathways. Prostaglandins Leukot. Essent. Fatty Acids 68: 181-186.
11. Yazawa, K. (1996) Production of eicosapentaenoic acid from marine bacteria. Lipids 31: S297-S300.
12. Morita, N., M. Tanaka, and H. Okuyama (2000) Biosynthesis of fatty acids in the docosahexaenoic acid-producing bacterium Moritella marina strain MP-1. Biochem. Soc. Trans. 28: 943-945.
13. Kaulmann, U. and C. Hertweck (2002) Biosynthesis of polyunsaturated fatty acids by polyketide synthases. Angew. Chem. Int. Ed. 41: 1866-1869.
14. Metz, J. G., P. Roessler, D. Facciotti, C. Levering, F. Dittrich, M. Lassner, R. Valentine, K. Lardizabal, F. Domergue, A. Yamada, K. Yazawa, V. Knauf, and J. Browse (2001) Production of polyunsaturated fatty acids by polyketide synthases in both prokaryotes and eukaryotes. Science 293: 290-293.
15. Abboud, R., R. Pora, V. Souza-Egipsy, C. S. Giometti, S. Tollaksen, J. J. Mosher, R. H. Findlay, and K. H. Nealson (2005) Low-temperature growth of Shewanella oneidensis MR-1. Appl. Environ. Microbiol. 71: 811-816.
16. Kato, C. and Y. Nogi (2001) Ccrrelation between phylogenetic structure and function: examples from deep-sea Shewanella. FEMS Microbiol. Ecol. 35: 223-230.
17. Jeong, Y.-S., S.-K. Song, S.-J. Lee, and B.-K. Hur (2006) The growth and EPA synthesis of Shewanella oneidensis MR-1 and expectation of EPA biosynthetic pathway. Biotechnol. Bioprocess Eng. 11: 127-133.
18. Orikasa, Y., A. Yamada, R. Yu, Y. Ito, T. Nishida, I. Yumoto, K. Watanabe, and H. Okuyama (2004) Characterization of the eicosapentaenoic acid biosynthesis gene cluster from Shewanella sp. strain SCRC-2738. Cell. Mol. Biol. 50: 625-630.
19. Choi, J.-I., S. Y. Lee, K. Shin, W. G. Lee, S. J. Park, H. N. Chang, and Y. K. Chang (2002) Pilot scale production of poly(3-hydroxybutyrate-co-3-hydroxy-valerate) by fed-batch culture of recombinant Escherichia coli. Biotechnol. Bioprocess Eng. 7: 371-374.
20. Choi, J.-I. and S. Y. Lee (2004) High level production of supra molecular weight poly(3-hydroxybutryrate) by metabolically engineered Escherichia coli. Biotechnol. Bioprocess Eng. 9: 196-200.
21. Yoon, S.-H., C. Li, Y.-M. Lee, S.-H. Lee, S.-H. Kim, M.-S. Choi, W.-T. Seo, J.-K. Yang, J.-Y. Kim, and S.-W. Kim (2005) Production of vanillin from ferulic acid using recombinant strains of Escherichia coli. Biotechnol. Bioprocess Eng. 10: 378-384.
22. Lepage, G. and C. C. Roy (1984) Improved recovery of fatty acid through direct transesterification without prior extraction or purification. J. Lipid Res. 25: 1391-1396.
23. Kang, K.-Y., D.-H. Alan, S.-M. Jung, D.-H. Kim, and B.-S. Chun (2005) Separation of protein and fatty acids from tuna viscera using supercritical carbon dioxide. Biotechnol. Bioprocess Eng. 10: 315-321.