Role of iron and sodium citrate in animal protein-free CHO cell culture medium on cell growth and monoclonal antibody production

Biotechnology Progress

Volumn 27, Issue 1, 2011, Pages 209-219

  Bai, Yunling ^a   Wu, Changjian ^a   Zhao, Jia ^a   Liu, Yan Hui ^a   Ding, Wei ^a   Ling, Wai Lam W ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Chemically defined medium; CHO cell culture; Iron; Monoclonal antibody; Sodium citrate

Indexed keywords

96-WELL PLATE; ANIMAL PROTEINS; ATP LEVEL; CELL LYSATES; CELLULAR ENERGY; CELLULAR PROTEINS; CHEMICAL PARTNERS; CHEMICALLY DEFINED MEDIUM; CHO CELL; CRITICAL COMPONENT; HEAVY AND LIGHT CHAINS; ION EXCHANGE CHROMATOGRAPHY; IRON CONCENTRATIONS; MONOCLONAL ANTIBODIES (MAB); MONOCLONAL ANTIBODY PRODUCTION; MULTIVESSEL; NORMAL PHASE; PRODUCT QUALITY; PROTEIN DEGRADATION; PROTEIN PRODUCTION; REVERSED PHASE; SHAKE FLASKS; SODIUM CITRATE;

ALLERGIES; AMINO ACIDS; ANIMALS; BOTTLES; CELL CULTURE; CELL GROWTH; CHROMATOGRAPHIC ANALYSIS; GLUCOSE; GROWTH KINETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ION CHROMATOGRAPHY; ION EXCHANGE; IRON COMPOUNDS; MACHINERY; MONOCLONAL ANTIBODIES; PROTEIN FOLDING; QUALITY CONTROL; SIZE EXCLUSION CHROMATOGRAPHY; SURFACE PLASMON RESONANCE;

ANIMAL CELL CULTURE;

CITRATE SODIUM; CITRIC ACID; FERRIC CITRATE; FERRIC ION; MONOCLONAL ANTIBODY;

ANIMAL; ARTICLE; BIOSYNTHESIS; CELL DIVISION; CRICETULUS; CULTURE MEDIUM; DRUG EFFECT; HAMSTER; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY;

ANIMALS; ANTIBODIES, MONOCLONAL; CELL DIVISION; CHROMATOGRAPHY, LIQUID; CITRATES; CRICETINAE; CRICETULUS; CULTURE MEDIA; FERRIC COMPOUNDS; MASS SPECTROMETRY;

ANIMALIA;

EID: 79851478058     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.513     Document Type: Article

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